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Electrogenerated chemiluminescence biosensor with alcohol dehydrogenase and tris(2,2'-bipyridyl)ruthenium (II) immobilized in sol-gel hybrid material.
Biosens Bioelectron. 2005 Sep 15; 21(3):455-61.BB

Abstract

An ethanol biosensor based on electrogenerated chemiluminescence detection was developed. Electrogenerated chemiluminescence reagent tris(2,2'-bipyridyl)ruthenium (II) and alcohol dehydrogenase were immobilized in the same sol-gel hybrid film. The copolymer poly(vinyl alcohol) with 4-vinylpyridine and cation exchanger Nafion were incorporated into sol-gel film to provide the microenvironment for retaining the activity of enzyme and immobilize tris(2,2'-bipyridyl)ruthenium (II). The design was simpler than the previous two-layer format. The experimental conditions, such as scan rate, pH and concentration of the cofactor were investigated. The intensity of electrogenerated chemiluminescence increased linearly with ethanol concentration from 2.5x10(-5) to 5.0x10(-2) M and detection limit was 1.0x10(-5) M. The prepared biosensor exhibited high sensitivity, wide linear range and good stability.

Authors+Show Affiliations

Xu Z
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
Guo Z
No affiliation info available
Dong S
No affiliation info available

MeSH

2,2'-DipyridylAlcohol DehydrogenaseBiosensing TechniquesCoordination ComplexesElectrochemistryEnzymes, ImmobilizedEquipment DesignEquipment Failure AnalysisEthanolLuminescent MeasurementsReproducibility of ResultsSensitivity and Specificity

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16076435

Citation

Xu, Zhiai, et al. "Electrogenerated Chemiluminescence Biosensor With Alcohol Dehydrogenase and Tris(2,2'-bipyridyl)ruthenium (II) Immobilized in Sol-gel Hybrid Material." Biosensors & Bioelectronics, vol. 21, no. 3, 2005, pp. 455-61.
Xu Z, Guo Z, Dong S. Electrogenerated chemiluminescence biosensor with alcohol dehydrogenase and tris(2,2'-bipyridyl)ruthenium (II) immobilized in sol-gel hybrid material. Biosens Bioelectron. 2005;21(3):455-61.
Xu, Z., Guo, Z., & Dong, S. (2005). Electrogenerated chemiluminescence biosensor with alcohol dehydrogenase and tris(2,2'-bipyridyl)ruthenium (II) immobilized in sol-gel hybrid material. Biosensors & Bioelectronics, 21(3), 455-61.
Xu Z, Guo Z, Dong S. Electrogenerated Chemiluminescence Biosensor With Alcohol Dehydrogenase and Tris(2,2'-bipyridyl)ruthenium (II) Immobilized in Sol-gel Hybrid Material. Biosens Bioelectron. 2005 Sep 15;21(3):455-61. PubMed PMID: 16076435.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Electrogenerated chemiluminescence biosensor with alcohol dehydrogenase and tris(2,2'-bipyridyl)ruthenium (II) immobilized in sol-gel hybrid material. AU - Xu,Zhiai, AU - Guo,Zhihui, AU - Dong,Shaojun, Y1 - 2004/12/18/ PY - 2004/07/27/received PY - 2004/10/26/revised PY - 2004/10/27/accepted PY - 2005/8/4/pubmed PY - 2006/2/16/medline PY - 2005/8/4/entrez SP - 455 EP - 61 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 21 IS - 3 N2 - An ethanol biosensor based on electrogenerated chemiluminescence detection was developed. Electrogenerated chemiluminescence reagent tris(2,2'-bipyridyl)ruthenium (II) and alcohol dehydrogenase were immobilized in the same sol-gel hybrid film. The copolymer poly(vinyl alcohol) with 4-vinylpyridine and cation exchanger Nafion were incorporated into sol-gel film to provide the microenvironment for retaining the activity of enzyme and immobilize tris(2,2'-bipyridyl)ruthenium (II). The design was simpler than the previous two-layer format. The experimental conditions, such as scan rate, pH and concentration of the cofactor were investigated. The intensity of electrogenerated chemiluminescence increased linearly with ethanol concentration from 2.5x10(-5) to 5.0x10(-2) M and detection limit was 1.0x10(-5) M. The prepared biosensor exhibited high sensitivity, wide linear range and good stability. SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/16076435/Electrogenerated_chemiluminescence_biosensor_with_alcohol_dehydrogenase_and_tris_22'_bipyridyl_ruthenium__II__immobilized_in_sol_gel_hybrid_material_ DB - PRIME DP - Unbound Medicine ER -