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Detection of pathogen Escherichia coli O157:H7 using self-excited PZT-glass microcantilevers.
Biosens Bioelectron. 2005 Sep 15; 21(3):462-73.BB

Abstract

Composite self-excited PZT-glass cantilevers (5 and 3 mm in length, 1.8 and 2.0 mm wide) were fabricated and their resonance characteristics were determined in air and at 1 mm liquid immersion. In air, resonance occurred at 65.8 and 63.4 kHz for the two cantilevers used in this paper. Monoclonal antibody (MAb) specific to the pathogen Escherichia coli (E. coli) O157:H7 was immobilized at the cantilever glass tip, and then exposed to pathogen in the concentration range of 7x10(2) to 7x10(7)bacteria/mL. Resonance of the second mode decreased due to pathogen attachment in accordance with a proposed kinetic model. The specific attachment rate constant was found to be 3x10(-9) to 5x10(-9) min-1 (cell/mL)-1. Exposure to a mixed population containing both a pathogenic and non-pathogenic strain showed that the antibody-immobilized cantilever is highly selective, thus demonstrating its usefulness for detecting water-borne pathogens.

Authors+Show Affiliations

Department of Chemical Engineering, Drexel University, Philadelphia, PA 19104, USA.No affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

16076436

Citation

Campbell, Gossett A., and Raj Mutharasan. "Detection of Pathogen Escherichia Coli O157:H7 Using Self-excited PZT-glass Microcantilevers." Biosensors & Bioelectronics, vol. 21, no. 3, 2005, pp. 462-73.
Campbell GA, Mutharasan R. Detection of pathogen Escherichia coli O157:H7 using self-excited PZT-glass microcantilevers. Biosens Bioelectron. 2005;21(3):462-73.
Campbell, G. A., & Mutharasan, R. (2005). Detection of pathogen Escherichia coli O157:H7 using self-excited PZT-glass microcantilevers. Biosensors & Bioelectronics, 21(3), 462-73.
Campbell GA, Mutharasan R. Detection of Pathogen Escherichia Coli O157:H7 Using Self-excited PZT-glass Microcantilevers. Biosens Bioelectron. 2005 Sep 15;21(3):462-73. PubMed PMID: 16076436.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of pathogen Escherichia coli O157:H7 using self-excited PZT-glass microcantilevers. AU - Campbell,Gossett A, AU - Mutharasan,Raj, Y1 - 2004/12/21/ PY - 2004/08/25/received PY - 2004/10/28/revised PY - 2004/11/12/accepted PY - 2005/8/4/pubmed PY - 2006/2/16/medline PY - 2005/8/4/entrez SP - 462 EP - 73 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 21 IS - 3 N2 - Composite self-excited PZT-glass cantilevers (5 and 3 mm in length, 1.8 and 2.0 mm wide) were fabricated and their resonance characteristics were determined in air and at 1 mm liquid immersion. In air, resonance occurred at 65.8 and 63.4 kHz for the two cantilevers used in this paper. Monoclonal antibody (MAb) specific to the pathogen Escherichia coli (E. coli) O157:H7 was immobilized at the cantilever glass tip, and then exposed to pathogen in the concentration range of 7x10(2) to 7x10(7)bacteria/mL. Resonance of the second mode decreased due to pathogen attachment in accordance with a proposed kinetic model. The specific attachment rate constant was found to be 3x10(-9) to 5x10(-9) min-1 (cell/mL)-1. Exposure to a mixed population containing both a pathogenic and non-pathogenic strain showed that the antibody-immobilized cantilever is highly selective, thus demonstrating its usefulness for detecting water-borne pathogens. SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/16076436/Detection_of_pathogen_Escherichia_coli_O157:H7_using_self_excited_PZT_glass_microcantilevers_ DB - PRIME DP - Unbound Medicine ER -