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Development and validation of a gas chromatography-mass spectrometry assay for opiates and cocaine in human teeth.
J Pharm Biomed Anal. 2006 Feb 24; 40(3):662-8.JP

Abstract

A procedure based on gas chromatography-mass spectrometry (GC-MS) is described for determination of opiates (6-monoacetylmorphine, morphine and codeine) and cocaine and metabolites (cocaine, benzoylecgonine and cocaethylene) in human teeth. After addition of nalorphine as internal standard, pulverized samples were incubated in HCl at 37 degrees C for 18 h. Then, after pH adjustment to 6, and the analytes were extracted with two volumes of 3 ml of chloroform/isopropanol (9:1). Chromatography was performed on a fused silica capillary column and analytes were determined in the selected-ion-monitoring (SIM) mode. The assay was validated in the range 7.5 (6.0 in case of codeine) to 500 ng/g with mean absolute recoveries ranged between 74.1 and 92.1% for the different analytes and precision and accuracy always better than 15%. The method was applied to the analysis of teeth from drug-addicts to assess past chronic consumption and verify self-reported declarations. In case of opiates, concentration range was 36.5-570.0 ng/g for 6-monoacetylmorphine, 8.7-154.8 ng/g for morphine and 7.9-127.9 ng/g for codeine. Cocaine concentration ranged between 5.6 and 57.2 ng/g with its principal metabolite benzoylecgonine varying from 12.6 to 81.7 ng/g and cocaethylene present in only one sample at 10 ng/g value. Teeth can be a promising non-invasive biological matrix in biomedical analysis for both clinical and forensic purposes.

Authors+Show Affiliations

Drug Research and Control Department, Istituto Superiore di Sanitá, V.le Regina Elena 299, 00161 Rome, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16076541

Citation

Pellegrini, Manuela, et al. "Development and Validation of a Gas Chromatography-mass Spectrometry Assay for Opiates and Cocaine in Human Teeth." Journal of Pharmaceutical and Biomedical Analysis, vol. 40, no. 3, 2006, pp. 662-8.
Pellegrini M, Casá A, Marchei E, et al. Development and validation of a gas chromatography-mass spectrometry assay for opiates and cocaine in human teeth. J Pharm Biomed Anal. 2006;40(3):662-8.
Pellegrini, M., Casá, A., Marchei, E., Pacifici, R., Mayné, R., Barbero, V., Garcia-Algar, O., & Pichini, S. (2006). Development and validation of a gas chromatography-mass spectrometry assay for opiates and cocaine in human teeth. Journal of Pharmaceutical and Biomedical Analysis, 40(3), 662-8.
Pellegrini M, et al. Development and Validation of a Gas Chromatography-mass Spectrometry Assay for Opiates and Cocaine in Human Teeth. J Pharm Biomed Anal. 2006 Feb 24;40(3):662-8. PubMed PMID: 16076541.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development and validation of a gas chromatography-mass spectrometry assay for opiates and cocaine in human teeth. AU - Pellegrini,Manuela, AU - Casá,Adriana, AU - Marchei,Emilia, AU - Pacifici,Roberta, AU - Mayné,Ruth, AU - Barbero,Vanessa, AU - Garcia-Algar,Oscar, AU - Pichini,Simona, Y1 - 2005/08/01/ PY - 2005/05/16/received PY - 2005/06/28/revised PY - 2005/07/01/accepted PY - 2005/8/4/pubmed PY - 2006/4/28/medline PY - 2005/8/4/entrez SP - 662 EP - 8 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 40 IS - 3 N2 - A procedure based on gas chromatography-mass spectrometry (GC-MS) is described for determination of opiates (6-monoacetylmorphine, morphine and codeine) and cocaine and metabolites (cocaine, benzoylecgonine and cocaethylene) in human teeth. After addition of nalorphine as internal standard, pulverized samples were incubated in HCl at 37 degrees C for 18 h. Then, after pH adjustment to 6, and the analytes were extracted with two volumes of 3 ml of chloroform/isopropanol (9:1). Chromatography was performed on a fused silica capillary column and analytes were determined in the selected-ion-monitoring (SIM) mode. The assay was validated in the range 7.5 (6.0 in case of codeine) to 500 ng/g with mean absolute recoveries ranged between 74.1 and 92.1% for the different analytes and precision and accuracy always better than 15%. The method was applied to the analysis of teeth from drug-addicts to assess past chronic consumption and verify self-reported declarations. In case of opiates, concentration range was 36.5-570.0 ng/g for 6-monoacetylmorphine, 8.7-154.8 ng/g for morphine and 7.9-127.9 ng/g for codeine. Cocaine concentration ranged between 5.6 and 57.2 ng/g with its principal metabolite benzoylecgonine varying from 12.6 to 81.7 ng/g and cocaethylene present in only one sample at 10 ng/g value. Teeth can be a promising non-invasive biological matrix in biomedical analysis for both clinical and forensic purposes. SN - 0731-7085 UR - https://www.unboundmedicine.com/medline/citation/16076541/Development_and_validation_of_a_gas_chromatography_mass_spectrometry_assay_for_opiates_and_cocaine_in_human_teeth_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(05)00436-X DB - PRIME DP - Unbound Medicine ER -