Tags

Type your tag names separated by a space and hit enter

High-level long-term white blood cell microchimerism after transfusion of leukoreduced blood components to patients resuscitated after severe traumatic injury.
Transfusion. 2005 Aug; 45(8):1280-90.T

Abstract

BACKGROUND

Long-term white blood cell (WBC) microchimerism (MC), of at least 2 years, has been reported in trauma patients receiving fresh nonleukoreduced (non-LR) blood. It is unknown, however, whether this occurs with LR blood products that are nearly devoid of WBCs. Twenty-seven patients transfused with LR and non-LR blood products were studied after severe traumatic injury. A secondary aim was to explore donor-recipient mixed lymphocyte reactivity in vitro.

STUDY DESIGN AND METHODS

To quantify MC, allele-specific real-time polymerase chain reaction assays were developed targeting HLA Class II sequence polymorphisms. Extensive validation showed that these assays reliably detect a single copy of target sequence in a complex allogeneic background without false positivity.

RESULTS

At a median follow-up of 26 months (range, 24-39 months), long-term MC was observed in 3 of 20 patients (15%) who received non-LR blood products and 2 of 7 (29%) who received LR blood products. The maximum MC ranged from 0.40 to 4.90 percent of circulating WBCs and appeared, by Class II genotype analysis, to be attributable to a single donor.

CONCLUSION

It is concluded that robust levels of long-term MC, apparently traceable to a single donor, occur at similar frequency despite leukoreduction of transfused blood products. Exploratory analysis of donor-recipient mixed lymphocyte reactivity suggests that long-term MC may require a state of bidirectional tolerance before transfusion.

Links

Publisher Full Text
onlinelibrary.wiley.com
doi.org
Aggregator Full Text

Authors+Show Affiliations

Lee TH
Blood Systems Research Institute, Department of Pathology, University of California, Davis, California 94118, USA. tlee@bloodsystems.org
Paglieroni T
No affiliation info available
Utter GH
No affiliation info available
Chafets D
No affiliation info available
Gosselin RC
No affiliation info available
Reed W
No affiliation info available
Owings JT
No affiliation info available
Holland PV
No affiliation info available
Busch MP
No affiliation info available

MeSH

Blood Component TransfusionBlood DonorsHLA-DR AntigensHumansLeukocyte Reduction ProceduresLymphocyte Culture Test, MixedSensitivity and SpecificityTransplantation ChimeraWounds and Injuries

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

16078913

Clinical Trial Links

Transfusion-Associated Microchimerism in Individuals Receiving a Blood Transfusion After a Traumatic Injury
Transfusion-Associated Microchimerism in Previously Injured Individuals Who Received a Blood Transfusion
Detailed Evaluation of Microchimerism

Citation

Lee, Tzong-Hae, et al. "High-level Long-term White Blood Cell Microchimerism After Transfusion of Leukoreduced Blood Components to Patients Resuscitated After Severe Traumatic Injury." Transfusion, vol. 45, no. 8, 2005, pp. 1280-90.
Lee TH, Paglieroni T, Utter GH, et al. High-level long-term white blood cell microchimerism after transfusion of leukoreduced blood components to patients resuscitated after severe traumatic injury. Transfusion. 2005;45(8):1280-90.
Lee, T. H., Paglieroni, T., Utter, G. H., Chafets, D., Gosselin, R. C., Reed, W., Owings, J. T., Holland, P. V., & Busch, M. P. (2005). High-level long-term white blood cell microchimerism after transfusion of leukoreduced blood components to patients resuscitated after severe traumatic injury. Transfusion, 45(8), 1280-90.
Lee TH, et al. High-level Long-term White Blood Cell Microchimerism After Transfusion of Leukoreduced Blood Components to Patients Resuscitated After Severe Traumatic Injury. Transfusion. 2005;45(8):1280-90. PubMed PMID: 16078913.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High-level long-term white blood cell microchimerism after transfusion of leukoreduced blood components to patients resuscitated after severe traumatic injury. AU - Lee,Tzong-Hae, AU - Paglieroni,Teresa, AU - Utter,Garth H, AU - Chafets,Daniel, AU - Gosselin,Robert C, AU - Reed,William, AU - Owings,John T, AU - Holland,Paul V, AU - Busch,Michael P, PY - 2005/8/5/pubmed PY - 2005/8/27/medline PY - 2005/8/5/entrez SP - 1280 EP - 90 JF - Transfusion JO - Transfusion VL - 45 IS - 8 N2 - BACKGROUND: Long-term white blood cell (WBC) microchimerism (MC), of at least 2 years, has been reported in trauma patients receiving fresh nonleukoreduced (non-LR) blood. It is unknown, however, whether this occurs with LR blood products that are nearly devoid of WBCs. Twenty-seven patients transfused with LR and non-LR blood products were studied after severe traumatic injury. A secondary aim was to explore donor-recipient mixed lymphocyte reactivity in vitro. STUDY DESIGN AND METHODS: To quantify MC, allele-specific real-time polymerase chain reaction assays were developed targeting HLA Class II sequence polymorphisms. Extensive validation showed that these assays reliably detect a single copy of target sequence in a complex allogeneic background without false positivity. RESULTS: At a median follow-up of 26 months (range, 24-39 months), long-term MC was observed in 3 of 20 patients (15%) who received non-LR blood products and 2 of 7 (29%) who received LR blood products. The maximum MC ranged from 0.40 to 4.90 percent of circulating WBCs and appeared, by Class II genotype analysis, to be attributable to a single donor. CONCLUSION: It is concluded that robust levels of long-term MC, apparently traceable to a single donor, occur at similar frequency despite leukoreduction of transfused blood products. Exploratory analysis of donor-recipient mixed lymphocyte reactivity suggests that long-term MC may require a state of bidirectional tolerance before transfusion. SN - 0041-1132 UR - https://www.unboundmedicine.com/medline/citation/16078913/High_level_long_term_white_blood_cell_microchimerism_after_transfusion_of_leukoreduced_blood_components_to_patients_resuscitated_after_severe_traumatic_injury_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0041-1132&date=2005&volume=45&issue=8&spage=1280 DB - PRIME DP - Unbound Medicine ER -