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High-level long-term white blood cell microchimerism after transfusion of leukoreduced blood components to patients resuscitated after severe traumatic injury.
Transfusion. 2005 Aug; 45(8):1280-90.T

Abstract

BACKGROUND

Long-term white blood cell (WBC) microchimerism (MC), of at least 2 years, has been reported in trauma patients receiving fresh nonleukoreduced (non-LR) blood. It is unknown, however, whether this occurs with LR blood products that are nearly devoid of WBCs. Twenty-seven patients transfused with LR and non-LR blood products were studied after severe traumatic injury. A secondary aim was to explore donor-recipient mixed lymphocyte reactivity in vitro.

STUDY DESIGN AND METHODS

To quantify MC, allele-specific real-time polymerase chain reaction assays were developed targeting HLA Class II sequence polymorphisms. Extensive validation showed that these assays reliably detect a single copy of target sequence in a complex allogeneic background without false positivity.

RESULTS

At a median follow-up of 26 months (range, 24-39 months), long-term MC was observed in 3 of 20 patients (15%) who received non-LR blood products and 2 of 7 (29%) who received LR blood products. The maximum MC ranged from 0.40 to 4.90 percent of circulating WBCs and appeared, by Class II genotype analysis, to be attributable to a single donor.

CONCLUSION

It is concluded that robust levels of long-term MC, apparently traceable to a single donor, occur at similar frequency despite leukoreduction of transfused blood products. Exploratory analysis of donor-recipient mixed lymphocyte reactivity suggests that long-term MC may require a state of bidirectional tolerance before transfusion.

Authors+Show Affiliations

Blood Systems Research Institute, Department of Pathology, University of California, Davis, California 94118, USA. tlee@bloodsystems.orgNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

16078913

Citation

Lee, Tzong-Hae, et al. "High-level Long-term White Blood Cell Microchimerism After Transfusion of Leukoreduced Blood Components to Patients Resuscitated After Severe Traumatic Injury." Transfusion, vol. 45, no. 8, 2005, pp. 1280-90.
Lee TH, Paglieroni T, Utter GH, et al. High-level long-term white blood cell microchimerism after transfusion of leukoreduced blood components to patients resuscitated after severe traumatic injury. Transfusion. 2005;45(8):1280-90.
Lee, T. H., Paglieroni, T., Utter, G. H., Chafets, D., Gosselin, R. C., Reed, W., Owings, J. T., Holland, P. V., & Busch, M. P. (2005). High-level long-term white blood cell microchimerism after transfusion of leukoreduced blood components to patients resuscitated after severe traumatic injury. Transfusion, 45(8), 1280-90.
Lee TH, et al. High-level Long-term White Blood Cell Microchimerism After Transfusion of Leukoreduced Blood Components to Patients Resuscitated After Severe Traumatic Injury. Transfusion. 2005;45(8):1280-90. PubMed PMID: 16078913.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High-level long-term white blood cell microchimerism after transfusion of leukoreduced blood components to patients resuscitated after severe traumatic injury. AU - Lee,Tzong-Hae, AU - Paglieroni,Teresa, AU - Utter,Garth H, AU - Chafets,Daniel, AU - Gosselin,Robert C, AU - Reed,William, AU - Owings,John T, AU - Holland,Paul V, AU - Busch,Michael P, PY - 2005/8/5/pubmed PY - 2005/8/27/medline PY - 2005/8/5/entrez SP - 1280 EP - 90 JF - Transfusion JO - Transfusion VL - 45 IS - 8 N2 - BACKGROUND: Long-term white blood cell (WBC) microchimerism (MC), of at least 2 years, has been reported in trauma patients receiving fresh nonleukoreduced (non-LR) blood. It is unknown, however, whether this occurs with LR blood products that are nearly devoid of WBCs. Twenty-seven patients transfused with LR and non-LR blood products were studied after severe traumatic injury. A secondary aim was to explore donor-recipient mixed lymphocyte reactivity in vitro. STUDY DESIGN AND METHODS: To quantify MC, allele-specific real-time polymerase chain reaction assays were developed targeting HLA Class II sequence polymorphisms. Extensive validation showed that these assays reliably detect a single copy of target sequence in a complex allogeneic background without false positivity. RESULTS: At a median follow-up of 26 months (range, 24-39 months), long-term MC was observed in 3 of 20 patients (15%) who received non-LR blood products and 2 of 7 (29%) who received LR blood products. The maximum MC ranged from 0.40 to 4.90 percent of circulating WBCs and appeared, by Class II genotype analysis, to be attributable to a single donor. CONCLUSION: It is concluded that robust levels of long-term MC, apparently traceable to a single donor, occur at similar frequency despite leukoreduction of transfused blood products. Exploratory analysis of donor-recipient mixed lymphocyte reactivity suggests that long-term MC may require a state of bidirectional tolerance before transfusion. SN - 0041-1132 UR - https://www.unboundmedicine.com/medline/citation/16078913/High_level_long_term_white_blood_cell_microchimerism_after_transfusion_of_leukoreduced_blood_components_to_patients_resuscitated_after_severe_traumatic_injury_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0041-1132&date=2005&volume=45&issue=8&spage=1280 DB - PRIME DP - Unbound Medicine ER -