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Separation and determination of toxic pyrrolizidine alkaloids in traditional Chinese herbal medicines by micellar electrokinetic chromatography with organic modifier.
Electrophoresis. 2005 Sep; 26(17):3397-404.E

Abstract

A simple and rapid micellar electrokinetic chromatography (MEKC) method was developed for the separation and determination of four toxic pyrrolizidine alkaloids (PAs) (senkirkine, senecionine, retrorsine, and seneciphylline) in two traditional Chinese herbal medicines (Qian liguang and Kuan donghua). Separation was performed in the running buffer consisting of 20 mM borate, 30 mM SDS, and 20% methanol at pH 9.1. With the optimized separation conditions, four PAs were separated in 17 min by a single run. The calibration curves showed good linearity with correlation efficiencies (R(2)) between 0.9940 and 0.9988. RSDs in migration time and peak area were 0.31, 0.40, 0.39, 0.48% and 3.28, 3.48, 4.16, 3.42% for senkirkine, senecionine, retrorsine, and seneciphylline, respectively. Limits of detection (S/N = 3) varied from 1.19 to 2.70 microg/mL. The proposed method was applied to determine the PAs extracted from Chinese herbal medicines (Qian liguang and Kuan donghua). PA of senkirkine in Kuan donghua was detected and the amount was found to be 79.1 microg/g. The results obtained indicate that the proposed MEKC method could potentially become an effective alternative tool for qualification control and quantitative analysis of herbal medicines in pharmaceutical industry.

Authors+Show Affiliations

Yu L
Department of Chemistry, National University of Singapore, Singapore, Republic of Singapore.
Xu Y
No affiliation info available
Feng H
No affiliation info available
Li SF
No affiliation info available

MeSH

BuffersCalibrationChromatography, High Pressure LiquidChromatography, Micellar Electrokinetic CapillaryDrugs, Chinese HerbalMethanolOrganic ChemicalsPyrrolizidine AlkaloidsReproducibility of ResultsSensitivity and SpecificitySodium Dodecyl Sulfate

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16080213

Citation

Yu, Lijun, et al. "Separation and Determination of Toxic Pyrrolizidine Alkaloids in Traditional Chinese Herbal Medicines By Micellar Electrokinetic Chromatography With Organic Modifier." Electrophoresis, vol. 26, no. 17, 2005, pp. 3397-404.
Yu L, Xu Y, Feng H, et al. Separation and determination of toxic pyrrolizidine alkaloids in traditional Chinese herbal medicines by micellar electrokinetic chromatography with organic modifier. Electrophoresis. 2005;26(17):3397-404.
Yu, L., Xu, Y., Feng, H., & Li, S. F. (2005). Separation and determination of toxic pyrrolizidine alkaloids in traditional Chinese herbal medicines by micellar electrokinetic chromatography with organic modifier. Electrophoresis, 26(17), 3397-404.
Yu L, et al. Separation and Determination of Toxic Pyrrolizidine Alkaloids in Traditional Chinese Herbal Medicines By Micellar Electrokinetic Chromatography With Organic Modifier. Electrophoresis. 2005;26(17):3397-404. PubMed PMID: 16080213.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Separation and determination of toxic pyrrolizidine alkaloids in traditional Chinese herbal medicines by micellar electrokinetic chromatography with organic modifier. AU - Yu,Lijun, AU - Xu,Yan, AU - Feng,Huatao, AU - Li,Sam Fong Yau, PY - 2005/8/5/pubmed PY - 2005/11/3/medline PY - 2005/8/5/entrez SP - 3397 EP - 404 JF - Electrophoresis JO - Electrophoresis VL - 26 IS - 17 N2 - A simple and rapid micellar electrokinetic chromatography (MEKC) method was developed for the separation and determination of four toxic pyrrolizidine alkaloids (PAs) (senkirkine, senecionine, retrorsine, and seneciphylline) in two traditional Chinese herbal medicines (Qian liguang and Kuan donghua). Separation was performed in the running buffer consisting of 20 mM borate, 30 mM SDS, and 20% methanol at pH 9.1. With the optimized separation conditions, four PAs were separated in 17 min by a single run. The calibration curves showed good linearity with correlation efficiencies (R(2)) between 0.9940 and 0.9988. RSDs in migration time and peak area were 0.31, 0.40, 0.39, 0.48% and 3.28, 3.48, 4.16, 3.42% for senkirkine, senecionine, retrorsine, and seneciphylline, respectively. Limits of detection (S/N = 3) varied from 1.19 to 2.70 microg/mL. The proposed method was applied to determine the PAs extracted from Chinese herbal medicines (Qian liguang and Kuan donghua). PA of senkirkine in Kuan donghua was detected and the amount was found to be 79.1 microg/g. The results obtained indicate that the proposed MEKC method could potentially become an effective alternative tool for qualification control and quantitative analysis of herbal medicines in pharmaceutical industry. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/16080213/Separation_and_determination_of_toxic_pyrrolizidine_alkaloids_in_traditional_Chinese_herbal_medicines_by_micellar_electrokinetic_chromatography_with_organic_modifier_ DB - PRIME DP - Unbound Medicine ER -