Tags

Type your tag names separated by a space and hit enter

Continuous fermentation of undetoxified dilute acid lignocellulose hydrolysate by Saccharomyces cerevisiae ATCC 96581 using cell recirculation.
Biotechnol Prog. 2005 Jul-Aug; 21(4):1093-101.BP

Abstract

Saccharomyces cerevisiae ATCC 96581 was cultivated in a chemostat reactor with undetoxified dilute acid softwood hydrolysate as the only carbon and energy source. The effects of nutrient addition, dilution rate, cell recirculation, and microaerobicity were investigated. Fermentation of unsupplemented dilute acid lignocellulose hydrolysate at D = 0.10 h(-1) in an anaerobic continuous reactor led to washout. Addition of ammonium sulfate or yeast extract was insufficient for obtaining steady state. In contrast, dilute acid lignocellulose hydrolysate supplemented with complete mineral medium, except for the carbon and energy source, was fermentable under anaerobic steady-state conditions at dilution rates up to 0.14 h(-1). Under these conditions, washout occurred at D = 0.15 h(-1). This was preceded by a drop in fermentative capacity and a very high specific ethanol production rate. Growth at all different dilution rates tested resulted in residual sugar in the chemostat. Cell recirculation (90%), achieved by cross-flow filtration, increased the sugar conversion rate from 92% to 99% at D = 0.10 h(-1). Nutrient addition clearly improved the long-term ethanol productivity in the recirculation cultures. Application of microaerobic conditions on the nutrient-supplemented recirculation cultures resulted in a higher production of biomass, a higher cellular protein content, and improved fermentative capacity, which further improves the robustness of fermentation of undetoxified lignocellulose hydrolysate.

Authors+Show Affiliations

Department of Chemical and Biological Engineering, Chalmers University of Technology, SE-412 96 Göteborg, Sweden.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16080688

Citation

Brandberg, Tomas, et al. "Continuous Fermentation of Undetoxified Dilute Acid Lignocellulose Hydrolysate By Saccharomyces Cerevisiae ATCC 96581 Using Cell Recirculation." Biotechnology Progress, vol. 21, no. 4, 2005, pp. 1093-101.
Brandberg T, Sanandaji N, Gustafsson L, et al. Continuous fermentation of undetoxified dilute acid lignocellulose hydrolysate by Saccharomyces cerevisiae ATCC 96581 using cell recirculation. Biotechnol Prog. 2005;21(4):1093-101.
Brandberg, T., Sanandaji, N., Gustafsson, L., & Franzén, C. J. (2005). Continuous fermentation of undetoxified dilute acid lignocellulose hydrolysate by Saccharomyces cerevisiae ATCC 96581 using cell recirculation. Biotechnology Progress, 21(4), 1093-101.
Brandberg T, et al. Continuous Fermentation of Undetoxified Dilute Acid Lignocellulose Hydrolysate By Saccharomyces Cerevisiae ATCC 96581 Using Cell Recirculation. Biotechnol Prog. 2005 Jul-Aug;21(4):1093-101. PubMed PMID: 16080688.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Continuous fermentation of undetoxified dilute acid lignocellulose hydrolysate by Saccharomyces cerevisiae ATCC 96581 using cell recirculation. AU - Brandberg,Tomas, AU - Sanandaji,Nima, AU - Gustafsson,Lena, AU - Franzén,Carl Johan, PY - 2005/8/6/pubmed PY - 2006/1/13/medline PY - 2005/8/6/entrez SP - 1093 EP - 101 JF - Biotechnology progress JO - Biotechnol Prog VL - 21 IS - 4 N2 - Saccharomyces cerevisiae ATCC 96581 was cultivated in a chemostat reactor with undetoxified dilute acid softwood hydrolysate as the only carbon and energy source. The effects of nutrient addition, dilution rate, cell recirculation, and microaerobicity were investigated. Fermentation of unsupplemented dilute acid lignocellulose hydrolysate at D = 0.10 h(-1) in an anaerobic continuous reactor led to washout. Addition of ammonium sulfate or yeast extract was insufficient for obtaining steady state. In contrast, dilute acid lignocellulose hydrolysate supplemented with complete mineral medium, except for the carbon and energy source, was fermentable under anaerobic steady-state conditions at dilution rates up to 0.14 h(-1). Under these conditions, washout occurred at D = 0.15 h(-1). This was preceded by a drop in fermentative capacity and a very high specific ethanol production rate. Growth at all different dilution rates tested resulted in residual sugar in the chemostat. Cell recirculation (90%), achieved by cross-flow filtration, increased the sugar conversion rate from 92% to 99% at D = 0.10 h(-1). Nutrient addition clearly improved the long-term ethanol productivity in the recirculation cultures. Application of microaerobic conditions on the nutrient-supplemented recirculation cultures resulted in a higher production of biomass, a higher cellular protein content, and improved fermentative capacity, which further improves the robustness of fermentation of undetoxified lignocellulose hydrolysate. SN - 8756-7938 UR - https://www.unboundmedicine.com/medline/citation/16080688/Continuous_fermentation_of_undetoxified_dilute_acid_lignocellulose_hydrolysate_by_Saccharomyces_cerevisiae_ATCC_96581_using_cell_recirculation_ L2 - https://doi.org/10.1021/bp050006y DB - PRIME DP - Unbound Medicine ER -