Tags

Type your tag names separated by a space and hit enter

Serum superoxide dismutase 3 (extracellular superoxide dismutase) activity is a sensitive indicator of Cu status in rats.
J Nutr Biochem 2005; 16(11):682-92JN

Abstract

Sensitivity of the assay for Cu,Zn superoxide dismutase 3 (SOD3), the predominant form of SOD in serum, can be increased, and interferences caused by low-molecular-weight substances in the serum can be reduced by conducting the assay at pH 10 with xanthine/xanthine oxidase and acetylated cytochrome c (cyt c) as superoxide generator and detector, respectively. Serum SOD3 activity was assayed under these conditions in an experiment where weanling, male rats were fed diets for 6 weeks containing 3, 5 and 15 mg Zn/kg with dietary Cu set at 0.3, 1.5 and 5 mg Cu/kg at each level of dietary Zn. Serum SOD3 responded to changes in dietary Cu but not to changes in dietary Zn. A second experiment compared serum SOD3 activity to traditional indices of Cu status in weanling, male and female rats after they were fed diets containing, nominally, 0, 1, 1.5, 2, 2.5, 3 and 6 mg Cu/kg for 6 weeks. Serum SOD3 activity was significantly lower (P < .05) in male rats fed diets containing 0 and 1 mg Cu/kg and female rats fed diet containing 0 mg Cu/kg compared with rats fed diet containing 6 mg Cu/kg. These changes were similar to changes in liver Cu concentrations, liver cyt c oxidase (CCO) activity and plasma ceruloplasmin in males and females. Serum SOD3 activity was also strongly, positively correlated with liver Cu concentrations over the entire range of dietary Cu concentrations (R(2) = .942 in males, R(2) = .884 in females, P < .0001). Plots of serum SOD3 activity, liver Cu concentration, liver CCO activity and ceruloplasmin as functions of kidney Cu concentration all had two linear segments that intersected at similar kidney Cu concentrations (18-22 microg/g dry kidney in males, 15-17 microg/g dry kidney in females). These findings indicate that serum SOD3 activity is a sensitive index of Cu status.

Authors+Show Affiliations

US Department of Agriculture, Agricultural Research Service, Grand Forks Human Nutrition Research Center, ND 58202-9034, USA. tjohnson@gfhnrc.ars.usda.govNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16081273

Citation

Johnson, W Thomas, et al. "Serum Superoxide Dismutase 3 (extracellular Superoxide Dismutase) Activity Is a Sensitive Indicator of Cu Status in Rats." The Journal of Nutritional Biochemistry, vol. 16, no. 11, 2005, pp. 682-92.
Johnson WT, Johnson LA, Lukaski HC. Serum superoxide dismutase 3 (extracellular superoxide dismutase) activity is a sensitive indicator of Cu status in rats. J Nutr Biochem. 2005;16(11):682-92.
Johnson, W. T., Johnson, L. A., & Lukaski, H. C. (2005). Serum superoxide dismutase 3 (extracellular superoxide dismutase) activity is a sensitive indicator of Cu status in rats. The Journal of Nutritional Biochemistry, 16(11), pp. 682-92.
Johnson WT, Johnson LA, Lukaski HC. Serum Superoxide Dismutase 3 (extracellular Superoxide Dismutase) Activity Is a Sensitive Indicator of Cu Status in Rats. J Nutr Biochem. 2005;16(11):682-92. PubMed PMID: 16081273.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Serum superoxide dismutase 3 (extracellular superoxide dismutase) activity is a sensitive indicator of Cu status in rats. AU - Johnson,W Thomas, AU - Johnson,Lu Ann K, AU - Lukaski,Henry C, Y1 - 2005/08/02/ PY - 2004/12/16/received PY - 2005/02/25/revised PY - 2005/03/14/accepted PY - 2005/8/6/pubmed PY - 2005/12/13/medline PY - 2005/8/6/entrez SP - 682 EP - 92 JF - The Journal of nutritional biochemistry JO - J. Nutr. Biochem. VL - 16 IS - 11 N2 - Sensitivity of the assay for Cu,Zn superoxide dismutase 3 (SOD3), the predominant form of SOD in serum, can be increased, and interferences caused by low-molecular-weight substances in the serum can be reduced by conducting the assay at pH 10 with xanthine/xanthine oxidase and acetylated cytochrome c (cyt c) as superoxide generator and detector, respectively. Serum SOD3 activity was assayed under these conditions in an experiment where weanling, male rats were fed diets for 6 weeks containing 3, 5 and 15 mg Zn/kg with dietary Cu set at 0.3, 1.5 and 5 mg Cu/kg at each level of dietary Zn. Serum SOD3 responded to changes in dietary Cu but not to changes in dietary Zn. A second experiment compared serum SOD3 activity to traditional indices of Cu status in weanling, male and female rats after they were fed diets containing, nominally, 0, 1, 1.5, 2, 2.5, 3 and 6 mg Cu/kg for 6 weeks. Serum SOD3 activity was significantly lower (P < .05) in male rats fed diets containing 0 and 1 mg Cu/kg and female rats fed diet containing 0 mg Cu/kg compared with rats fed diet containing 6 mg Cu/kg. These changes were similar to changes in liver Cu concentrations, liver cyt c oxidase (CCO) activity and plasma ceruloplasmin in males and females. Serum SOD3 activity was also strongly, positively correlated with liver Cu concentrations over the entire range of dietary Cu concentrations (R(2) = .942 in males, R(2) = .884 in females, P < .0001). Plots of serum SOD3 activity, liver Cu concentration, liver CCO activity and ceruloplasmin as functions of kidney Cu concentration all had two linear segments that intersected at similar kidney Cu concentrations (18-22 microg/g dry kidney in males, 15-17 microg/g dry kidney in females). These findings indicate that serum SOD3 activity is a sensitive index of Cu status. SN - 0955-2863 UR - https://www.unboundmedicine.com/medline/citation/16081273/Serum_superoxide_dismutase_3__extracellular_superoxide_dismutase__activity_is_a_sensitive_indicator_of_Cu_status_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0955-2863(05)00081-1 DB - PRIME DP - Unbound Medicine ER -