Tags

Type your tag names separated by a space and hit enter

Distinct diversity of vacA, cagA, and cagE genes of Helicobacter pylori associated with peptic ulcer in Japan.
J Clin Microbiol. 2005 Aug; 43(8):3906-16.JC

Abstract

Colonization of the stomach mucosa by Helicobacter pylori is a major cause of acute and chronic gastric pathologies in humans. Several H. pylori virulence genes that may play a role in its pathogenicity have been identified. The most important determinants are vacA and cagA in the cag pathogenicity island (cagPAI) genes. In the present study, to consider the association of molecular genetics between vacA and the cagPAI regarding clinical outcome, we selected H. pylori strains with various genotypes of vacA in Japan and sequenced full-length vacA, cagA, and cagE genes. Sequencing of vacA and cagA genes revealed variable size, whereas the cagE gene was well conserved among strains. Each of the phylogenetic trees based on the deduced amino acid sequences of VacA, CagA, and CagE indicated that all three proteins were divided into two major groups, a Western group and an East Asian group, and the distributions of isolates exhibited similar patterns among the three proteins. The strains with s2 and s1a/m1a vacA genotypes and the Western-type 3' region cagA genotype were classified into the Western group, and the strains with the s1c/m1b vacA genotype and the East Asian-type 3' cagA genotype were included in the East Asian group. In addition, the prevalence of infection with the Western group strain was significantly higher in patients with peptic ulcer (90.0%, 9/10) than in patients with chronic gastritis (22.7%, 5/22) (chi2 = 12.64, P = 0.00057). These data suggest that the molecular genetics of vacA and cagPAI are associated and that the Western group with vacA and cagPAI genes is associated with peptic ulcer disease.

Authors+Show Affiliations

Second Department of Internal Medicine, Faculty of Medical Sciences, University of Fukui, Fukui, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16081930

Citation

Yamazaki, Shiho, et al. "Distinct Diversity of vacA, cagA, and cagE Genes of Helicobacter Pylori Associated With Peptic Ulcer in Japan." Journal of Clinical Microbiology, vol. 43, no. 8, 2005, pp. 3906-16.
Yamazaki S, Yamakawa A, Okuda T, et al. Distinct diversity of vacA, cagA, and cagE genes of Helicobacter pylori associated with peptic ulcer in Japan. J Clin Microbiol. 2005;43(8):3906-16.
Yamazaki, S., Yamakawa, A., Okuda, T., Ohtani, M., Suto, H., Ito, Y., Yamazaki, Y., Keida, Y., Higashi, H., Hatakeyama, M., & Azuma, T. (2005). Distinct diversity of vacA, cagA, and cagE genes of Helicobacter pylori associated with peptic ulcer in Japan. Journal of Clinical Microbiology, 43(8), 3906-16.
Yamazaki S, et al. Distinct Diversity of vacA, cagA, and cagE Genes of Helicobacter Pylori Associated With Peptic Ulcer in Japan. J Clin Microbiol. 2005;43(8):3906-16. PubMed PMID: 16081930.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Distinct diversity of vacA, cagA, and cagE genes of Helicobacter pylori associated with peptic ulcer in Japan. AU - Yamazaki,Shiho, AU - Yamakawa,Akiyo, AU - Okuda,Tomoyuki, AU - Ohtani,Masahiro, AU - Suto,Hiroyuki, AU - Ito,Yoshiyuki, AU - Yamazaki,Yukinao, AU - Keida,Yoshihide, AU - Higashi,Hideaki, AU - Hatakeyama,Masanori, AU - Azuma,Takeshi, PY - 2005/8/6/pubmed PY - 2005/9/16/medline PY - 2005/8/6/entrez SP - 3906 EP - 16 JF - Journal of clinical microbiology JO - J Clin Microbiol VL - 43 IS - 8 N2 - Colonization of the stomach mucosa by Helicobacter pylori is a major cause of acute and chronic gastric pathologies in humans. Several H. pylori virulence genes that may play a role in its pathogenicity have been identified. The most important determinants are vacA and cagA in the cag pathogenicity island (cagPAI) genes. In the present study, to consider the association of molecular genetics between vacA and the cagPAI regarding clinical outcome, we selected H. pylori strains with various genotypes of vacA in Japan and sequenced full-length vacA, cagA, and cagE genes. Sequencing of vacA and cagA genes revealed variable size, whereas the cagE gene was well conserved among strains. Each of the phylogenetic trees based on the deduced amino acid sequences of VacA, CagA, and CagE indicated that all three proteins were divided into two major groups, a Western group and an East Asian group, and the distributions of isolates exhibited similar patterns among the three proteins. The strains with s2 and s1a/m1a vacA genotypes and the Western-type 3' region cagA genotype were classified into the Western group, and the strains with the s1c/m1b vacA genotype and the East Asian-type 3' cagA genotype were included in the East Asian group. In addition, the prevalence of infection with the Western group strain was significantly higher in patients with peptic ulcer (90.0%, 9/10) than in patients with chronic gastritis (22.7%, 5/22) (chi2 = 12.64, P = 0.00057). These data suggest that the molecular genetics of vacA and cagPAI are associated and that the Western group with vacA and cagPAI genes is associated with peptic ulcer disease. SN - 0095-1137 UR - https://www.unboundmedicine.com/medline/citation/16081930/Distinct_diversity_of_vacA_cagA_and_cagE_genes_of_Helicobacter_pylori_associated_with_peptic_ulcer_in_Japan_ L2 - https://journals.asm.org/doi/10.1128/JCM.43.8.3906-3916.2005?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -