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[Expression of signal transducer and activator of transcription 6 in rat asthma model and the modulatory effect of dexamethasone].
Zhonghua Er Ke Za Zhi. 2005 Jul; 43(7):521-5.ZE

Abstract

OBJECTIVE

To study the expression of signal transducer and activator of transcription 6 and its mRNA in rat asthma model and the modulatory effect of dexamethasone (DXM).

METHODS

Thirty male SD rats were randomly divided into three groups: the control group, asthma group and DXM group. The rats in each group were sacrificed 24 h after the last challenge. In the experiment, the rat model of asthma was established by ovalbumin (OVA) challenge method. The lung tissue was taken from the left lung, and bronchoalveolar lavage fluid (BALF) was collected from the right lung. The total cell numbers, eosinophils (EOS) count and differentiated cell counts in BALF were performed on different count fluids. The concentrations of IL-4 in serum and BALF were measured by using sandwich ELISA. The protein expressions of STAT6 were detected with immunohistochemical techniques. The mRNA expressions of STAT6 were detected with in situ hybridization.

RESULTS

(1) The total cell counts in BALF, the absolute counts of EOS, and the ratios of eosinophils to the total cell numbers (EOS%) of asthma group were all significantly higher than those of the control group (P < 0.01). The total cell counts in BALF, the absolute counts of EOS, and EOS% of DXM group were all significantly lower than those of asthma group (P < 0.01). (2) The concentrations of IL-4 in BALF and serum of asthma group [(25.7 +/- 7.4) ng/L, (34.2 +/- 10.5) ng/L] were significantly higher than those of control group [(8.6 +/- 3.0) ng/L, (12.1 +/- 2.9) ng/L] (P < 0.01). The concentrations of IL-4 in BALF and serum of DXM group were significantly lower than those of asthma group. (3) Immunohistochemistry showed that the protein content of STAT6 around the bronchus of asthma group (0.171 +/- 0.025) was significantly higher than that of the control group (0.082 +/- 0.022) (P < 0.01), while that of DXM group (0.114 +/- 0.013) was significantly lower than that of asthma group. The epithelial cells were the cells. In situ hybridization showed that the mRNA expression of STAT6 around the bronchus of asthma group (0.180 +/- 0.013) was significantly higher than that of the control group (0.091 +/- 0.012) (P < 0.01), while that of DXM group (0.114 +/- 0.010) was significantly lower than that of asthma group. (4) There was a significant correlation between the concentration of IL-4 in BALF, the content of STAT6 and STAT6 mRNA, respectively, in the epithelial cells of bronchus. There was a significant correlation between the absolute numbers of EOS and EOS% in BALF, the content of STAT6 and STAT6 mRNA, respectively, in the epithelial cells of bronchus.

CONCLUSIONS

STAT6 protein and STAT6 mRNA were found strongly expressed in rat asthma model and the epithelial cells were the chief expressing cells. Dexamethasone had an inhibitory effect on airway inflammatory cells infiltration. It significantly depressed STAT6 and mRNA expression. Which may be a key process in modulatory mechanism of asthma.

Authors+Show Affiliations

Department of Respiratory Medicine, Yuying Children's Hospital Affiliated to Wenzhou Medical College, Wenzhou 325027, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

16083555

Citation

Li, Chang-chong, et al. "[Expression of Signal Transducer and Activator of Transcription 6 in Rat Asthma Model and the Modulatory Effect of Dexamethasone]." Zhonghua Er Ke Za Zhi = Chinese Journal of Pediatrics, vol. 43, no. 7, 2005, pp. 521-5.
Li CC, Ye LP, Chen XF, et al. [Expression of signal transducer and activator of transcription 6 in rat asthma model and the modulatory effect of dexamethasone]. Zhonghua Er Ke Za Zhi. 2005;43(7):521-5.
Li, C. C., Ye, L. P., Chen, X. F., Li, S. B., Cai, X. H., Dong, L., Luo, Y. C., & Zhang, Z. X. (2005). [Expression of signal transducer and activator of transcription 6 in rat asthma model and the modulatory effect of dexamethasone]. Zhonghua Er Ke Za Zhi = Chinese Journal of Pediatrics, 43(7), 521-5.
Li CC, et al. [Expression of Signal Transducer and Activator of Transcription 6 in Rat Asthma Model and the Modulatory Effect of Dexamethasone]. Zhonghua Er Ke Za Zhi. 2005;43(7):521-5. PubMed PMID: 16083555.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Expression of signal transducer and activator of transcription 6 in rat asthma model and the modulatory effect of dexamethasone]. AU - Li,Chang-chong, AU - Ye,Le-ping, AU - Chen,Xiao-fang, AU - Li,Shao-bo, AU - Cai,Xiao-hong, AU - Dong,Lin, AU - Luo,Yun-chun, AU - Zhang,Zheng-xia, PY - 2005/8/9/pubmed PY - 2010/7/21/medline PY - 2005/8/9/entrez SP - 521 EP - 5 JF - Zhonghua er ke za zhi = Chinese journal of pediatrics JO - Zhonghua Er Ke Za Zhi VL - 43 IS - 7 N2 - OBJECTIVE: To study the expression of signal transducer and activator of transcription 6 and its mRNA in rat asthma model and the modulatory effect of dexamethasone (DXM). METHODS: Thirty male SD rats were randomly divided into three groups: the control group, asthma group and DXM group. The rats in each group were sacrificed 24 h after the last challenge. In the experiment, the rat model of asthma was established by ovalbumin (OVA) challenge method. The lung tissue was taken from the left lung, and bronchoalveolar lavage fluid (BALF) was collected from the right lung. The total cell numbers, eosinophils (EOS) count and differentiated cell counts in BALF were performed on different count fluids. The concentrations of IL-4 in serum and BALF were measured by using sandwich ELISA. The protein expressions of STAT6 were detected with immunohistochemical techniques. The mRNA expressions of STAT6 were detected with in situ hybridization. RESULTS: (1) The total cell counts in BALF, the absolute counts of EOS, and the ratios of eosinophils to the total cell numbers (EOS%) of asthma group were all significantly higher than those of the control group (P < 0.01). The total cell counts in BALF, the absolute counts of EOS, and EOS% of DXM group were all significantly lower than those of asthma group (P < 0.01). (2) The concentrations of IL-4 in BALF and serum of asthma group [(25.7 +/- 7.4) ng/L, (34.2 +/- 10.5) ng/L] were significantly higher than those of control group [(8.6 +/- 3.0) ng/L, (12.1 +/- 2.9) ng/L] (P < 0.01). The concentrations of IL-4 in BALF and serum of DXM group were significantly lower than those of asthma group. (3) Immunohistochemistry showed that the protein content of STAT6 around the bronchus of asthma group (0.171 +/- 0.025) was significantly higher than that of the control group (0.082 +/- 0.022) (P < 0.01), while that of DXM group (0.114 +/- 0.013) was significantly lower than that of asthma group. The epithelial cells were the cells. In situ hybridization showed that the mRNA expression of STAT6 around the bronchus of asthma group (0.180 +/- 0.013) was significantly higher than that of the control group (0.091 +/- 0.012) (P < 0.01), while that of DXM group (0.114 +/- 0.010) was significantly lower than that of asthma group. (4) There was a significant correlation between the concentration of IL-4 in BALF, the content of STAT6 and STAT6 mRNA, respectively, in the epithelial cells of bronchus. There was a significant correlation between the absolute numbers of EOS and EOS% in BALF, the content of STAT6 and STAT6 mRNA, respectively, in the epithelial cells of bronchus. CONCLUSIONS: STAT6 protein and STAT6 mRNA were found strongly expressed in rat asthma model and the epithelial cells were the chief expressing cells. Dexamethasone had an inhibitory effect on airway inflammatory cells infiltration. It significantly depressed STAT6 and mRNA expression. Which may be a key process in modulatory mechanism of asthma. SN - 0578-1310 UR - https://www.unboundmedicine.com/medline/citation/16083555/[Expression_of_signal_transducer_and_activator_of_transcription_6_in_rat_asthma_model_and_the_modulatory_effect_of_dexamethasone]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&amp;issn=0578-1310&amp;year=2005&amp;vol=43&amp;issue=7&amp;fpage=521 DB - PRIME DP - Unbound Medicine ER -