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Modelling inactivation of Listeria monocytogenes by pulsed electric fields in media of different pH.
Int J Food Microbiol. 2005 Aug 25; 103(2):199-206.IJ

Abstract

A study of the effect of square-wave pulsed electric fields (PEF) on the inactivation of Listeria monocytogenes in McIlvaine buffer of different pH (3.5-7.0) was conducted. L. monocytoges was more PEF sensitive at higher electric field strengths (E) and in media of low pH. A treatment at 28 kV/cm for 400 mus that inactivated 1.5, 2.3 and 3.0 Log10 cycles at pH 7.0, 6.5 and 5.0 respectively destroyed almost 6.0 Log10 cycles at pH 3.5. The general shape of survival curves of L. monocytogenes PEF treated at different pH was convex/concave upwards. A mathematical model based on the Weibull distribution accurately described these survival curves. At each pH, the shape parameter (n value) did not depend on E. The relationship between n value of the Weibull model and the pH of the treatment medium was described by the Gompertz equation. A multiple linear regression model using three predictor variables (E, E2, pH2) related the Log10 of the scale paramenter (b value) of the Weibull model with E and pH of the treatment medium. A tertiary model developed using McIlvaine buffer as treatment medium predicted satisfactorily the inactivation of L. monocytogenes in apple juice.

Authors+Show Affiliations

Gómez N
Tecnología de los Alimentos, Facultad de Veterinaria, Universidad de Zaragoza, Miguel Servet 177, 50.013 Zaragoza, Spain.
García D
No affiliation info available
Alvarez I
No affiliation info available
Condón S
No affiliation info available
Raso J
No affiliation info available

MeSH

Colony Count, MicrobialConsumer Product SafetyElectric ConductivityElectric StimulationFood MicrobiologyFood PreservationHydrogen-Ion ConcentrationListeria monocytogenesMathematicsModels, BiologicalTime Factors

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16083822

Citation

Gómez, N, et al. "Modelling Inactivation of Listeria Monocytogenes By Pulsed Electric Fields in Media of Different PH." International Journal of Food Microbiology, vol. 103, no. 2, 2005, pp. 199-206.
Gómez N, García D, Alvarez I, et al. Modelling inactivation of Listeria monocytogenes by pulsed electric fields in media of different pH. Int J Food Microbiol. 2005;103(2):199-206.
Gómez, N., García, D., Alvarez, I., Condón, S., & Raso, J. (2005). Modelling inactivation of Listeria monocytogenes by pulsed electric fields in media of different pH. International Journal of Food Microbiology, 103(2), 199-206.
Gómez N, et al. Modelling Inactivation of Listeria Monocytogenes By Pulsed Electric Fields in Media of Different PH. Int J Food Microbiol. 2005 Aug 25;103(2):199-206. PubMed PMID: 16083822.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Modelling inactivation of Listeria monocytogenes by pulsed electric fields in media of different pH. AU - Gómez,N, AU - García,D, AU - Alvarez,I, AU - Condón,S, AU - Raso,J, PY - 2004/01/19/received PY - 2004/09/06/revised PY - 2004/11/25/accepted PY - 2005/8/9/pubmed PY - 2005/9/30/medline PY - 2005/8/9/entrez SP - 199 EP - 206 JF - International journal of food microbiology JO - Int J Food Microbiol VL - 103 IS - 2 N2 - A study of the effect of square-wave pulsed electric fields (PEF) on the inactivation of Listeria monocytogenes in McIlvaine buffer of different pH (3.5-7.0) was conducted. L. monocytoges was more PEF sensitive at higher electric field strengths (E) and in media of low pH. A treatment at 28 kV/cm for 400 mus that inactivated 1.5, 2.3 and 3.0 Log10 cycles at pH 7.0, 6.5 and 5.0 respectively destroyed almost 6.0 Log10 cycles at pH 3.5. The general shape of survival curves of L. monocytogenes PEF treated at different pH was convex/concave upwards. A mathematical model based on the Weibull distribution accurately described these survival curves. At each pH, the shape parameter (n value) did not depend on E. The relationship between n value of the Weibull model and the pH of the treatment medium was described by the Gompertz equation. A multiple linear regression model using three predictor variables (E, E2, pH2) related the Log10 of the scale paramenter (b value) of the Weibull model with E and pH of the treatment medium. A tertiary model developed using McIlvaine buffer as treatment medium predicted satisfactorily the inactivation of L. monocytogenes in apple juice. SN - 0168-1605 UR - https://www.unboundmedicine.com/medline/citation/16083822/Modelling_inactivation_of_Listeria_monocytogenes_by_pulsed_electric_fields_in_media_of_different_pH_ DB - PRIME DP - Unbound Medicine ER -