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The hematopoietic transcription factor PU.1 regulates RANK gene expression in myeloid progenitors.
Biochem Biophys Res Commun. 2005 Sep 23; 335(2):437-46.BB

Abstract

Osteoclasts are bone resorbing cells of hematopoietic origin. The hematopoietic transcription factor PU.1 is critical for osteoclastogenesis; however, the molecular mechanisms of PU.1-regulated osteoclastogenesis have not been explored. Here, we present evidence that the receptor activator of nuclear factor kappaB (RANK) gene that has been shown to be crucial for osteoclastogenesis is a transcriptional target of PU.1. The PU.1-/- progenitor cells failed to express the RANK gene and reconstitution of PU.1 in these cells induced RANK expression. Treatment of the PU.1 reconstituted cells with M-CSF and RANKL further augmented the RANK gene expression. To explore the regulatory mechanism of the RANK gene expression by PU.1, we have cloned the human RANK promoter. Transient transfection assays have revealed that the 2.2-kb RANK promoter was functional in a monocyte line RAW264.7, whereas co-transfection of PU.1 transactivated the RANK promoter in HeLa cells. Taken together, these results suggest that PU.1 regulates the RANK gene transcription and this may represent one of the key roles of PU.1 in osteoclast differentiation.

Authors+Show Affiliations

Systemic Proteomics Research Center, Korea Research Institute of Bioscience and Biotechnology, 52 Oun-dong, Yusong, Daejeon 305-333, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16083856

Citation

Kwon, Oh Hyung, et al. "The Hematopoietic Transcription Factor PU.1 Regulates RANK Gene Expression in Myeloid Progenitors." Biochemical and Biophysical Research Communications, vol. 335, no. 2, 2005, pp. 437-46.
Kwon OH, Lee CK, Lee YI, et al. The hematopoietic transcription factor PU.1 regulates RANK gene expression in myeloid progenitors. Biochem Biophys Res Commun. 2005;335(2):437-46.
Kwon, O. H., Lee, C. K., Lee, Y. I., Paik, S. G., & Lee, H. J. (2005). The hematopoietic transcription factor PU.1 regulates RANK gene expression in myeloid progenitors. Biochemical and Biophysical Research Communications, 335(2), 437-46.
Kwon OH, et al. The Hematopoietic Transcription Factor PU.1 Regulates RANK Gene Expression in Myeloid Progenitors. Biochem Biophys Res Commun. 2005 Sep 23;335(2):437-46. PubMed PMID: 16083856.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The hematopoietic transcription factor PU.1 regulates RANK gene expression in myeloid progenitors. AU - Kwon,Oh Hyung, AU - Lee,Chong-Kil, AU - Lee,Young Ik, AU - Paik,Sang-Gi, AU - Lee,Hyun-Jun, PY - 2005/07/13/received PY - 2005/07/16/accepted PY - 2005/8/9/pubmed PY - 2005/10/15/medline PY - 2005/8/9/entrez SP - 437 EP - 46 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 335 IS - 2 N2 - Osteoclasts are bone resorbing cells of hematopoietic origin. The hematopoietic transcription factor PU.1 is critical for osteoclastogenesis; however, the molecular mechanisms of PU.1-regulated osteoclastogenesis have not been explored. Here, we present evidence that the receptor activator of nuclear factor kappaB (RANK) gene that has been shown to be crucial for osteoclastogenesis is a transcriptional target of PU.1. The PU.1-/- progenitor cells failed to express the RANK gene and reconstitution of PU.1 in these cells induced RANK expression. Treatment of the PU.1 reconstituted cells with M-CSF and RANKL further augmented the RANK gene expression. To explore the regulatory mechanism of the RANK gene expression by PU.1, we have cloned the human RANK promoter. Transient transfection assays have revealed that the 2.2-kb RANK promoter was functional in a monocyte line RAW264.7, whereas co-transfection of PU.1 transactivated the RANK promoter in HeLa cells. Taken together, these results suggest that PU.1 regulates the RANK gene transcription and this may represent one of the key roles of PU.1 in osteoclast differentiation. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/16083856/The_hematopoietic_transcription_factor_PU_1_regulates_RANK_gene_expression_in_myeloid_progenitors_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(05)01567-6 DB - PRIME DP - Unbound Medicine ER -