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A chronic dietary toxicity study of DAG (diacylglycerol) in Beagle dogs.
Food Chem Toxicol. 2006 Jan; 44(1):81-97.FC

Abstract

The potential chronic toxic effects of DAG (diacylglycerol) when administered orally for 12 months were evaluated in this dietary study in Beagle dogs. DAG is a cooking oil which contains >80% diglycerides, <20% triglycerides and 5% monoglycerides. For this study, a special diet was prepared with no dietary fat so that all of the dietary fat could be provided by DAG, at various concentrations together with a control oil. The control oil, TG (triacylglycerol), was prepared to contain >85% triglycerides, <10% diglycerides and 5% monoglycerides. The fatty acid composition for DAG and TG was closely matched. Dietary concentrations of 0% DAG/9.5% TG (TG control), 1.5% DAG/8.0% TG, 5.5% DAG/4.0% TG, and 9.5% DAG/0% TG were presented daily, seven days per week, for 52 weeks. A second concurrent control group received the standard basal diet (Certified Canine LabDiet 5007, which has a fat content of 9.5%). The basal diet, control article-treated and DAG-treated groups each consisted of four male and four female dogs. Treatment was initiated in prejuvenile (2.5-month-old) dogs. Statistical evaluations compared the DAG-treated groups both to the basal diet and 9.5% TG control groups. The clinical condition of the animals, body weights, body weight gains and food consumption were unaffected by DAG. Hematology and urinalysis parameters were unaffected. No serum chemistry changes indicative of a toxic effect were observed. There were no effects noted on ECG data. No test article-related gross or histopathologic findings or changes in organ weights were observed. While there were no identifiable differences between the effects of TG and DAG, both caused some differences relative to the basal diet (lower food consumption, higher alkaline phosphatase, cholesterol and triglycerides). These differences were not toxicologically significant and were attributed to the differences in the diet rather than the fat source. Thus, DAG at dietary concentrations up to 9.5% for one year had no effect on normal canine growth and development, in comparison to TG.

Authors+Show Affiliations

Chengelis CP
WIL Research Laboratories, LLC, Ashland, OH 44805-9281, USA. chengelis@wilresearch.com
Kirkpatrick JB
No affiliation info available
Marit GB
No affiliation info available
Morita O
No affiliation info available
Tamaki Y
No affiliation info available
Suzuki H
No affiliation info available

MeSH

Administration, OralAnimalsBody WeightClinical Chemistry TestsDietDiglyceridesDogsDose-Response Relationship, DrugEatingFatty AcidsFemaleFood, FormulatedHematologic TestsMaleToxicity Tests, ChronicTriglycerides

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16084638

Citation

Chengelis, Christopher P., et al. "A Chronic Dietary Toxicity Study of DAG (diacylglycerol) in Beagle Dogs." Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, vol. 44, no. 1, 2006, pp. 81-97.
Chengelis CP, Kirkpatrick JB, Marit GB, et al. A chronic dietary toxicity study of DAG (diacylglycerol) in Beagle dogs. Food Chem Toxicol. 2006;44(1):81-97.
Chengelis, C. P., Kirkpatrick, J. B., Marit, G. B., Morita, O., Tamaki, Y., & Suzuki, H. (2006). A chronic dietary toxicity study of DAG (diacylglycerol) in Beagle dogs. Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, 44(1), 81-97.
Chengelis CP, et al. A Chronic Dietary Toxicity Study of DAG (diacylglycerol) in Beagle Dogs. Food Chem Toxicol. 2006;44(1):81-97. PubMed PMID: 16084638.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A chronic dietary toxicity study of DAG (diacylglycerol) in Beagle dogs. AU - Chengelis,Christopher P, AU - Kirkpatrick,Jeannie B, AU - Marit,Gary B, AU - Morita,Osamu, AU - Tamaki,Yasushi, AU - Suzuki,Hiroyuki, Y1 - 2005/08/09/ PY - 2004/01/22/received PY - 2005/05/19/revised PY - 2005/06/17/accepted PY - 2005/8/9/pubmed PY - 2006/2/10/medline PY - 2005/8/9/entrez SP - 81 EP - 97 JF - Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association JO - Food Chem Toxicol VL - 44 IS - 1 N2 - The potential chronic toxic effects of DAG (diacylglycerol) when administered orally for 12 months were evaluated in this dietary study in Beagle dogs. DAG is a cooking oil which contains >80% diglycerides, <20% triglycerides and 5% monoglycerides. For this study, a special diet was prepared with no dietary fat so that all of the dietary fat could be provided by DAG, at various concentrations together with a control oil. The control oil, TG (triacylglycerol), was prepared to contain >85% triglycerides, <10% diglycerides and 5% monoglycerides. The fatty acid composition for DAG and TG was closely matched. Dietary concentrations of 0% DAG/9.5% TG (TG control), 1.5% DAG/8.0% TG, 5.5% DAG/4.0% TG, and 9.5% DAG/0% TG were presented daily, seven days per week, for 52 weeks. A second concurrent control group received the standard basal diet (Certified Canine LabDiet 5007, which has a fat content of 9.5%). The basal diet, control article-treated and DAG-treated groups each consisted of four male and four female dogs. Treatment was initiated in prejuvenile (2.5-month-old) dogs. Statistical evaluations compared the DAG-treated groups both to the basal diet and 9.5% TG control groups. The clinical condition of the animals, body weights, body weight gains and food consumption were unaffected by DAG. Hematology and urinalysis parameters were unaffected. No serum chemistry changes indicative of a toxic effect were observed. There were no effects noted on ECG data. No test article-related gross or histopathologic findings or changes in organ weights were observed. While there were no identifiable differences between the effects of TG and DAG, both caused some differences relative to the basal diet (lower food consumption, higher alkaline phosphatase, cholesterol and triglycerides). These differences were not toxicologically significant and were attributed to the differences in the diet rather than the fat source. Thus, DAG at dietary concentrations up to 9.5% for one year had no effect on normal canine growth and development, in comparison to TG. SN - 0278-6915 UR - https://www.unboundmedicine.com/medline/citation/16084638/A_chronic_dietary_toxicity_study_of_DAG__diacylglycerol__in_Beagle_dogs_ DB - PRIME DP - Unbound Medicine ER -