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Separation and determination of pseudoephedrine, dextromethorphan, diphenhydramine and chlorpheniramine in cold medicines by nonaqueous capillary electrophoresis.
J Pharm Biomed Anal. 2005 Sep 01; 39(1-2):285-9.JP

Abstract

An easy, rapid and simple nonaqueous capillary electrophoresis (NACE) method was developed for the identification and determination of four basic nitrogenous compounds, i.e. pseudoephedrine (PE), dextromethorphan (DXM), diphenhydramine (DHM) and chlorpheniramine (CLP). The most suitable running buffer was composed of 40 mM ammonium acetate, 10% acetonitrile (ACN) in methanol with a fused-silica capillary column (47 cm x 75 microm i.d.), 25 kV applied voltage and 25 degrees C capillary temperature. The calibration curves revealed linear relationships between the peak area for each analyte and its concentration (correlation coefficients: 0.9993 for PE, 0.9971 for DXM, 0.9991 for DHM, and 0.9995 for CLP, respectively). The relative standard deviations of the migration time and peak area of the four compounds were 0.37, 3.90, 0.73 and 0.68, and 2.80, 3.50, 1.60 and 3.70%, respectively. The method was successfully applied to determine the four compounds in five cold medicines, the recoveries of the four constituents ranging between 91 and 109%.

Authors+Show Affiliations

Department of Chemistry, Lanzhou University, PR China. dongym03@st.lzu.edu.cnNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16085144

Citation

Dong, Yuming, et al. "Separation and Determination of Pseudoephedrine, Dextromethorphan, Diphenhydramine and Chlorpheniramine in Cold Medicines By Nonaqueous Capillary Electrophoresis." Journal of Pharmaceutical and Biomedical Analysis, vol. 39, no. 1-2, 2005, pp. 285-9.
Dong Y, Chen X, Chen Y, et al. Separation and determination of pseudoephedrine, dextromethorphan, diphenhydramine and chlorpheniramine in cold medicines by nonaqueous capillary electrophoresis. J Pharm Biomed Anal. 2005;39(1-2):285-9.
Dong, Y., Chen, X., Chen, Y., Chen, X., & Hu, Z. (2005). Separation and determination of pseudoephedrine, dextromethorphan, diphenhydramine and chlorpheniramine in cold medicines by nonaqueous capillary electrophoresis. Journal of Pharmaceutical and Biomedical Analysis, 39(1-2), 285-9.
Dong Y, et al. Separation and Determination of Pseudoephedrine, Dextromethorphan, Diphenhydramine and Chlorpheniramine in Cold Medicines By Nonaqueous Capillary Electrophoresis. J Pharm Biomed Anal. 2005 Sep 1;39(1-2):285-9. PubMed PMID: 16085144.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Separation and determination of pseudoephedrine, dextromethorphan, diphenhydramine and chlorpheniramine in cold medicines by nonaqueous capillary electrophoresis. AU - Dong,Yuming, AU - Chen,Xiaofeng, AU - Chen,Yonglei, AU - Chen,Xingguo, AU - Hu,Zhide, Y1 - 2005/04/02/ PY - 2005/01/07/received PY - 2005/02/15/revised PY - 2005/02/25/accepted PY - 2005/8/9/pubmed PY - 2005/11/16/medline PY - 2005/8/9/entrez SP - 285 EP - 9 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 39 IS - 1-2 N2 - An easy, rapid and simple nonaqueous capillary electrophoresis (NACE) method was developed for the identification and determination of four basic nitrogenous compounds, i.e. pseudoephedrine (PE), dextromethorphan (DXM), diphenhydramine (DHM) and chlorpheniramine (CLP). The most suitable running buffer was composed of 40 mM ammonium acetate, 10% acetonitrile (ACN) in methanol with a fused-silica capillary column (47 cm x 75 microm i.d.), 25 kV applied voltage and 25 degrees C capillary temperature. The calibration curves revealed linear relationships between the peak area for each analyte and its concentration (correlation coefficients: 0.9993 for PE, 0.9971 for DXM, 0.9991 for DHM, and 0.9995 for CLP, respectively). The relative standard deviations of the migration time and peak area of the four compounds were 0.37, 3.90, 0.73 and 0.68, and 2.80, 3.50, 1.60 and 3.70%, respectively. The method was successfully applied to determine the four compounds in five cold medicines, the recoveries of the four constituents ranging between 91 and 109%. SN - 0731-7085 UR - https://www.unboundmedicine.com/medline/citation/16085144/Separation_and_determination_of_pseudoephedrine_dextromethorphan_diphenhydramine_and_chlorpheniramine_in_cold_medicines_by_nonaqueous_capillary_electrophoresis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(05)00154-8 DB - PRIME DP - Unbound Medicine ER -