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PIP2 hydrolysis and calcium release are required for cytokinesis in Drosophila spermatocytes.
Curr Biol. 2005 Aug 09; 15(15):1401-6.CB

Abstract

The role of calcium (Ca(2+)) in cytokinesis is controversial, and the precise pathways that lead to its release during cleavage are not well understood. Ca(2+) is released from intracellular stores by binding of inositol trisphosphate (IP3) to the IP3 receptor (IP3R), yet no clear role in cytokinesis has been established for the precursor of IP3, phosphatidylinositol 4,5-bisphosphate (PIP2). Here, using transgenic flies expressing PLCdelta-PH-GFP, which specifically binds PIP2, we identify PIP2 in the plasma membrane and cleavage furrows of dividing Drosophila melanogaster spermatocytes, and we establish that this phospholipid is required for continued ingression but not for initiation of cytokinesis. In addition, by inhibiting phospholipase C, we show that PIP2 must be hydrolyzed to maintain cleavage furrow stability. Using an IP3R antagonist and a Ca(2+) chelator to examine the roles of IP3R and Ca(2+) in cytokinesis, we demonstrate that both of these factors are required for cleavage furrow stability, although Ca(2+) is dispensable for cleavage plane specification and initiation of furrowing. Strikingly, providing cells with Ca(2+) obviates the need to hydrolyze PIP2. Thus, PIP2, PIP2 hydrolysis, and Ca(2+) are required for the normal progression of cytokinesis in these cells.

Authors+Show Affiliations

Program in Developmental Biology, The Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16085493

Citation

Wong, Raymond, et al. "PIP2 Hydrolysis and Calcium Release Are Required for Cytokinesis in Drosophila Spermatocytes." Current Biology : CB, vol. 15, no. 15, 2005, pp. 1401-6.
Wong R, Hadjiyanni I, Wei HC, et al. PIP2 hydrolysis and calcium release are required for cytokinesis in Drosophila spermatocytes. Curr Biol. 2005;15(15):1401-6.
Wong, R., Hadjiyanni, I., Wei, H. C., Polevoy, G., McBride, R., Sem, K. P., & Brill, J. A. (2005). PIP2 hydrolysis and calcium release are required for cytokinesis in Drosophila spermatocytes. Current Biology : CB, 15(15), 1401-6.
Wong R, et al. PIP2 Hydrolysis and Calcium Release Are Required for Cytokinesis in Drosophila Spermatocytes. Curr Biol. 2005 Aug 9;15(15):1401-6. PubMed PMID: 16085493.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - PIP2 hydrolysis and calcium release are required for cytokinesis in Drosophila spermatocytes. AU - Wong,Raymond, AU - Hadjiyanni,Irene, AU - Wei,Ho-Chun, AU - Polevoy,Gordon, AU - McBride,Rachel, AU - Sem,Kai-Ping, AU - Brill,Julie A, PY - 2005/02/02/received PY - 2005/06/15/revised PY - 2005/06/16/accepted PY - 2005/8/9/pubmed PY - 2006/1/6/medline PY - 2005/8/9/entrez SP - 1401 EP - 6 JF - Current biology : CB JO - Curr. Biol. VL - 15 IS - 15 N2 - The role of calcium (Ca(2+)) in cytokinesis is controversial, and the precise pathways that lead to its release during cleavage are not well understood. Ca(2+) is released from intracellular stores by binding of inositol trisphosphate (IP3) to the IP3 receptor (IP3R), yet no clear role in cytokinesis has been established for the precursor of IP3, phosphatidylinositol 4,5-bisphosphate (PIP2). Here, using transgenic flies expressing PLCdelta-PH-GFP, which specifically binds PIP2, we identify PIP2 in the plasma membrane and cleavage furrows of dividing Drosophila melanogaster spermatocytes, and we establish that this phospholipid is required for continued ingression but not for initiation of cytokinesis. In addition, by inhibiting phospholipase C, we show that PIP2 must be hydrolyzed to maintain cleavage furrow stability. Using an IP3R antagonist and a Ca(2+) chelator to examine the roles of IP3R and Ca(2+) in cytokinesis, we demonstrate that both of these factors are required for cleavage furrow stability, although Ca(2+) is dispensable for cleavage plane specification and initiation of furrowing. Strikingly, providing cells with Ca(2+) obviates the need to hydrolyze PIP2. Thus, PIP2, PIP2 hydrolysis, and Ca(2+) are required for the normal progression of cytokinesis in these cells. SN - 0960-9822 UR - https://www.unboundmedicine.com/medline/citation/16085493/PIP2_hydrolysis_and_calcium_release_are_required_for_cytokinesis_in_Drosophila_spermatocytes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0960-9822(05)00709-8 DB - PRIME DP - Unbound Medicine ER -