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PtdIns(4,5)P2 functions at the cleavage furrow during cytokinesis.
Curr Biol 2005; 15(15):1407-12CB

Abstract

Phosphoinositides play important roles in regulating the cytoskeleton and vesicle trafficking, potentially important processes at the cleavage furrow. However, it remains unclear which, if any, of the phosphoinositides play a role during cytokinesis. A systematic analysis to determine if any of the phosphoinositides might be present or of functional importance at the cleavage furrow has not been published. Several studies hint at a possible role for one or more phosphoinositides at the cleavage furrow. The best of these are genetic data identifying mutations in phosphoinositide-modifying enzymes (a PtdIns(4)P-5-kinase in S. pombe and a PI-4-kinase in D. melanogaster) that interfere with cytokinesis. The genetic nature of these experiments leaves questions as to how direct may be their contribution to cytokinesis. Here we show that a single phosphoinositide, PtdIns(4,5)P2, specifically accumulates at the furrow. Interference with PtdIns(4,5)P2 interferes with adhesion of the plasma membrane to the contractile ring at the furrow. Finally, four distinct interventions to specifically interfere with PtdIns(4,5)P2 each impair cytokinesis. We conclude that PtdIns(4,5)P2 is present at the cleavage furrow and is required for normal cytokinesis at least in part because of a role in adhesion between the contractile ring and the plasma membrane.

Authors+Show Affiliations

Division of Signal Transduction, Beth Israel-Deaconess Medical Center, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA. sjfield@ucsd.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

16085494

Citation

Field, Seth J., et al. "PtdIns(4,5)P2 Functions at the Cleavage Furrow During Cytokinesis." Current Biology : CB, vol. 15, no. 15, 2005, pp. 1407-12.
Field SJ, Madson N, Kerr ML, et al. PtdIns(4,5)P2 functions at the cleavage furrow during cytokinesis. Curr Biol. 2005;15(15):1407-12.
Field, S. J., Madson, N., Kerr, M. L., Galbraith, K. A., Kennedy, C. E., Tahiliani, M., ... Cantley, L. C. (2005). PtdIns(4,5)P2 functions at the cleavage furrow during cytokinesis. Current Biology : CB, 15(15), pp. 1407-12.
Field SJ, et al. PtdIns(4,5)P2 Functions at the Cleavage Furrow During Cytokinesis. Curr Biol. 2005 Aug 9;15(15):1407-12. PubMed PMID: 16085494.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - PtdIns(4,5)P2 functions at the cleavage furrow during cytokinesis. AU - Field,Seth J, AU - Madson,Nikki, AU - Kerr,Monica L, AU - Galbraith,Kenneth A A, AU - Kennedy,Caitlin E, AU - Tahiliani,Mamta, AU - Wilkins,Andrew, AU - Cantley,Lewis C, PY - 2005/02/02/received PY - 2005/06/21/revised PY - 2005/06/22/accepted PY - 2005/8/9/pubmed PY - 2006/1/6/medline PY - 2005/8/9/entrez SP - 1407 EP - 12 JF - Current biology : CB JO - Curr. Biol. VL - 15 IS - 15 N2 - Phosphoinositides play important roles in regulating the cytoskeleton and vesicle trafficking, potentially important processes at the cleavage furrow. However, it remains unclear which, if any, of the phosphoinositides play a role during cytokinesis. A systematic analysis to determine if any of the phosphoinositides might be present or of functional importance at the cleavage furrow has not been published. Several studies hint at a possible role for one or more phosphoinositides at the cleavage furrow. The best of these are genetic data identifying mutations in phosphoinositide-modifying enzymes (a PtdIns(4)P-5-kinase in S. pombe and a PI-4-kinase in D. melanogaster) that interfere with cytokinesis. The genetic nature of these experiments leaves questions as to how direct may be their contribution to cytokinesis. Here we show that a single phosphoinositide, PtdIns(4,5)P2, specifically accumulates at the furrow. Interference with PtdIns(4,5)P2 interferes with adhesion of the plasma membrane to the contractile ring at the furrow. Finally, four distinct interventions to specifically interfere with PtdIns(4,5)P2 each impair cytokinesis. We conclude that PtdIns(4,5)P2 is present at the cleavage furrow and is required for normal cytokinesis at least in part because of a role in adhesion between the contractile ring and the plasma membrane. SN - 0960-9822 UR - https://www.unboundmedicine.com/medline/citation/16085494/PtdIns_45_P2_functions_at_the_cleavage_furrow_during_cytokinesis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0960-9822(05)00707-4 DB - PRIME DP - Unbound Medicine ER -