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Activation of the protein kinase C1 pathway upon continuous heat stress in Saccharomyces cerevisiae is triggered by an intracellular increase in osmolarity due to trehalose accumulation.
Appl Environ Microbiol. 2005 Aug; 71(8):4531-8.AE

Abstract

This paper reports on physiological and molecular responses of Saccharomyces cerevisiae to heat stress conditions. We observed that within a very narrow range of culture temperatures, a shift from exponential growth to growth arrest and ultimately to cell death occurred. A detailed analysis was carried out of the accumulation of trehalose and the activation of the protein kinase C1 (PKC1) (cell integrity) pathway in both glucose- and ethanol-grown cells upon temperature upshifts within this narrow range of growth temperatures. It was observed that the PKC1 pathway was hardly activated in a tps1 mutant that is unable to accumulate any trehalose. Furthermore, it was observed that an increase of the extracellular osmolarity during a continuous heat stress prevented the activation of the pathway. The results of these analyses support our hypothesis that under heat stress conditions the activation of the PKC1 pathway is triggered by an increase in intracellular osmolarity, due to the accumulation of trehalose, rather than by the increase in temperature as such.

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Authors+Show Affiliations

Mensonides FI
Swammerdam Institute of Life Sciences, Department of Molecular Microbial Physiology, University of Amsterdam, Nieuwe Achtergracht 166, 1018 WV Amsterdam, The Netherlands.
Brul S
No affiliation info available
Klis FM
No affiliation info available
Hellingwerf KJ
No affiliation info available
Teixeira de Mattos MJ
No affiliation info available

MeSH

Culture MediaGene Expression Regulation, FungalGlucosyltransferasesHeat-Shock ResponseOsmolar ConcentrationProtein Kinase CSaccharomyces cerevisiaeTemperatureTrehalose

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16085846

Citation

Mensonides, Femke I C., et al. "Activation of the Protein Kinase C1 Pathway Upon Continuous Heat Stress in Saccharomyces Cerevisiae Is Triggered By an Intracellular Increase in Osmolarity Due to Trehalose Accumulation." Applied and Environmental Microbiology, vol. 71, no. 8, 2005, pp. 4531-8.
Mensonides FI, Brul S, Klis FM, et al. Activation of the protein kinase C1 pathway upon continuous heat stress in Saccharomyces cerevisiae is triggered by an intracellular increase in osmolarity due to trehalose accumulation. Appl Environ Microbiol. 2005;71(8):4531-8.
Mensonides, F. I., Brul, S., Klis, F. M., Hellingwerf, K. J., & Teixeira de Mattos, M. J. (2005). Activation of the protein kinase C1 pathway upon continuous heat stress in Saccharomyces cerevisiae is triggered by an intracellular increase in osmolarity due to trehalose accumulation. Applied and Environmental Microbiology, 71(8), 4531-8.
Mensonides FI, et al. Activation of the Protein Kinase C1 Pathway Upon Continuous Heat Stress in Saccharomyces Cerevisiae Is Triggered By an Intracellular Increase in Osmolarity Due to Trehalose Accumulation. Appl Environ Microbiol. 2005;71(8):4531-8. PubMed PMID: 16085846.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Activation of the protein kinase C1 pathway upon continuous heat stress in Saccharomyces cerevisiae is triggered by an intracellular increase in osmolarity due to trehalose accumulation. AU - Mensonides,Femke I C, AU - Brul,Stanley, AU - Klis,Frans M, AU - Hellingwerf,Klaas J, AU - Teixeira de Mattos,M Joost, PY - 2005/8/9/pubmed PY - 2005/10/26/medline PY - 2005/8/9/entrez SP - 4531 EP - 8 JF - Applied and environmental microbiology JO - Appl Environ Microbiol VL - 71 IS - 8 N2 - This paper reports on physiological and molecular responses of Saccharomyces cerevisiae to heat stress conditions. We observed that within a very narrow range of culture temperatures, a shift from exponential growth to growth arrest and ultimately to cell death occurred. A detailed analysis was carried out of the accumulation of trehalose and the activation of the protein kinase C1 (PKC1) (cell integrity) pathway in both glucose- and ethanol-grown cells upon temperature upshifts within this narrow range of growth temperatures. It was observed that the PKC1 pathway was hardly activated in a tps1 mutant that is unable to accumulate any trehalose. Furthermore, it was observed that an increase of the extracellular osmolarity during a continuous heat stress prevented the activation of the pathway. The results of these analyses support our hypothesis that under heat stress conditions the activation of the PKC1 pathway is triggered by an increase in intracellular osmolarity, due to the accumulation of trehalose, rather than by the increase in temperature as such. SN - 0099-2240 UR - https://www.unboundmedicine.com/medline/citation/16085846/Activation_of_the_protein_kinase_C1_pathway_upon_continuous_heat_stress_in_Saccharomyces_cerevisiae_is_triggered_by_an_intracellular_increase_in_osmolarity_due_to_trehalose_accumulation_ DB - PRIME DP - Unbound Medicine ER -