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Inhibition of Colorado potato beetle larvae by a locust proteinase inhibitor peptide expressed in potato.
Biotechnol Lett. 2005 Jun; 27(12):829-34.BL

Abstract

The cDNA for a 73-mer peptide containing two locust serine proteinase inhibitors was cloned, fused to the constitutive CaMV35S promoter and introduced into potato by Agrobacterium-mediated transformation. From 23 independent transgenic lines, three with high mRNA level and proteinase inhibitory activity were propagated in vitro and transferred to pots. The peptide from the leaves was identified by its N-terminal sequence and by K(i) values against chymotrypsin and trypsin. Colorado potato beetle larvae reared on transgenic plants grew slightly but significantly more slowly than those on control plants. This supports the notion that expression of multifunctional proteinase inhibitors of insect origin might be a good strategy to improve insect resistance in plants.

Authors+Show Affiliations

Kondrák M
Institute of Plant Biology, Agricultural Biotechnology Center, Gödöllo, Hungary.
Kutas J
No affiliation info available
Szenthe B
No affiliation info available
Patthy A
No affiliation info available
Bánfalvi Z
No affiliation info available
Nádasy M
No affiliation info available
Gráf L
No affiliation info available
Asbóth B
No affiliation info available

MeSH

AnimalsBlotting, NorthernCloning, MolecularColeopteraFeeding BehaviorGene ExpressionGrasshoppersInsect ProteinsLarvaPlant LeavesPlants, Genetically ModifiedProtease InhibitorsSequence Analysis, ProteinSolanum tuberosumTime Factors

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16086243

Citation

Kondrák, Mihály, et al. "Inhibition of Colorado Potato Beetle Larvae By a Locust Proteinase Inhibitor Peptide Expressed in Potato." Biotechnology Letters, vol. 27, no. 12, 2005, pp. 829-34.
Kondrák M, Kutas J, Szenthe B, et al. Inhibition of Colorado potato beetle larvae by a locust proteinase inhibitor peptide expressed in potato. Biotechnol Lett. 2005;27(12):829-34.
Kondrák, M., Kutas, J., Szenthe, B., Patthy, A., Bánfalvi, Z., Nádasy, M., Gráf, L., & Asbóth, B. (2005). Inhibition of Colorado potato beetle larvae by a locust proteinase inhibitor peptide expressed in potato. Biotechnology Letters, 27(12), 829-34.
Kondrák M, et al. Inhibition of Colorado Potato Beetle Larvae By a Locust Proteinase Inhibitor Peptide Expressed in Potato. Biotechnol Lett. 2005;27(12):829-34. PubMed PMID: 16086243.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibition of Colorado potato beetle larvae by a locust proteinase inhibitor peptide expressed in potato. AU - Kondrák,Mihály, AU - Kutas,János, AU - Szenthe,Borbála, AU - Patthy,András, AU - Bánfalvi,Zsófia, AU - Nádasy,Miklós, AU - Gráf,László, AU - Asbóth,Bence, PY - 2005/01/20/received PY - 2005/04/16/accepted PY - 2005/04/12/revised PY - 2005/8/9/pubmed PY - 2005/10/18/medline PY - 2005/8/9/entrez SP - 829 EP - 34 JF - Biotechnology letters JO - Biotechnol Lett VL - 27 IS - 12 N2 - The cDNA for a 73-mer peptide containing two locust serine proteinase inhibitors was cloned, fused to the constitutive CaMV35S promoter and introduced into potato by Agrobacterium-mediated transformation. From 23 independent transgenic lines, three with high mRNA level and proteinase inhibitory activity were propagated in vitro and transferred to pots. The peptide from the leaves was identified by its N-terminal sequence and by K(i) values against chymotrypsin and trypsin. Colorado potato beetle larvae reared on transgenic plants grew slightly but significantly more slowly than those on control plants. This supports the notion that expression of multifunctional proteinase inhibitors of insect origin might be a good strategy to improve insect resistance in plants. SN - 0141-5492 UR - https://www.unboundmedicine.com/medline/citation/16086243/Inhibition_of_Colorado_potato_beetle_larvae_by_a_locust_proteinase_inhibitor_peptide_expressed_in_potato_ DB - PRIME DP - Unbound Medicine ER -