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Substrate availability and transcriptional regulation of metabolic genes in human skeletal muscle during recovery from exercise.
Metabolism. 2005 Aug; 54(8):1048-55.M

Abstract

In skeletal muscle of humans, transcription of several metabolic genes is transiently induced during recovery from exercise when no food is consumed. To determine the potential influence of substrate availability on the transcriptional regulation of metabolic genes during recovery from exercise, 9 male subjects (aged 22-27) completed 75 minutes of cycling exercise at 75% Vo2 max on 2 occasions, consuming either a high-carbohydrate (HC) or low-carbohydrate (LC) diet during the subsequent 24 hours of recovery. Nuclei were isolated and tissue frozen from vastus lateralis muscle biopsies obtained before exercise and 2, 5, 8, and 24 hours after exercise. Muscle glycogen was restored to near resting levels within 5 hours in the HC trial, but remained depressed through 24 hours in the LC trial. During the 2- to 8-hour recovery period, leg glucose uptake was 5- to 15-fold higher with HC ingestion, whereas arterial plasma free fatty acid levels were approximately 3- to 7-fold higher with LC ingestion. Exercise increased (P < .05) transcription and/or mRNA content of the pyruvate dehydrogenase kinase 4, uncoupling protein 3, lipoprotein lipase, carnitine palmitoyltransferase I, hexokinase II, peroxisome proliferator activated receptor gamma coactivator-1 alpha, and peroxisome proliferator activated receptor alpha. Providing HC during recovery reversed the activation of pyruvate dehydrogenase kinase 4, uncoupling protein 3, lipoprotein lipase, and carnitine palmitoyltransferase I within 5 to 8 hours after exercise, whereas providing LC during recovery elicited a sustained/enhanced increase in activation of these genes through 8 to 24 hours of recovery. These findings provide evidence that factors associated with substrate availability and/or cellular metabolic recovery (eg, muscle glycogen restoration) influence the transcriptional regulation of metabolic genes in skeletal muscle of humans during recovery from exercise.

Authors+Show Affiliations

Copenhagen Muscle Research Centre, University of Copenhagen, Copenhagen, Denmark.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

16092055

Citation

Pilegaard, Henriette, et al. "Substrate Availability and Transcriptional Regulation of Metabolic Genes in Human Skeletal Muscle During Recovery From Exercise." Metabolism: Clinical and Experimental, vol. 54, no. 8, 2005, pp. 1048-55.
Pilegaard H, Osada T, Andersen LT, et al. Substrate availability and transcriptional regulation of metabolic genes in human skeletal muscle during recovery from exercise. Metab Clin Exp. 2005;54(8):1048-55.
Pilegaard, H., Osada, T., Andersen, L. T., Helge, J. W., Saltin, B., & Neufer, P. D. (2005). Substrate availability and transcriptional regulation of metabolic genes in human skeletal muscle during recovery from exercise. Metabolism: Clinical and Experimental, 54(8), 1048-55.
Pilegaard H, et al. Substrate Availability and Transcriptional Regulation of Metabolic Genes in Human Skeletal Muscle During Recovery From Exercise. Metab Clin Exp. 2005;54(8):1048-55. PubMed PMID: 16092055.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Substrate availability and transcriptional regulation of metabolic genes in human skeletal muscle during recovery from exercise. AU - Pilegaard,Henriette, AU - Osada,Takuya, AU - Andersen,Lisbeth T, AU - Helge,Jørn W, AU - Saltin,Bengt, AU - Neufer,P Darrell, PY - 2005/8/11/pubmed PY - 2005/10/12/medline PY - 2005/8/11/entrez SP - 1048 EP - 55 JF - Metabolism: clinical and experimental JO - Metab. Clin. Exp. VL - 54 IS - 8 N2 - In skeletal muscle of humans, transcription of several metabolic genes is transiently induced during recovery from exercise when no food is consumed. To determine the potential influence of substrate availability on the transcriptional regulation of metabolic genes during recovery from exercise, 9 male subjects (aged 22-27) completed 75 minutes of cycling exercise at 75% Vo2 max on 2 occasions, consuming either a high-carbohydrate (HC) or low-carbohydrate (LC) diet during the subsequent 24 hours of recovery. Nuclei were isolated and tissue frozen from vastus lateralis muscle biopsies obtained before exercise and 2, 5, 8, and 24 hours after exercise. Muscle glycogen was restored to near resting levels within 5 hours in the HC trial, but remained depressed through 24 hours in the LC trial. During the 2- to 8-hour recovery period, leg glucose uptake was 5- to 15-fold higher with HC ingestion, whereas arterial plasma free fatty acid levels were approximately 3- to 7-fold higher with LC ingestion. Exercise increased (P < .05) transcription and/or mRNA content of the pyruvate dehydrogenase kinase 4, uncoupling protein 3, lipoprotein lipase, carnitine palmitoyltransferase I, hexokinase II, peroxisome proliferator activated receptor gamma coactivator-1 alpha, and peroxisome proliferator activated receptor alpha. Providing HC during recovery reversed the activation of pyruvate dehydrogenase kinase 4, uncoupling protein 3, lipoprotein lipase, and carnitine palmitoyltransferase I within 5 to 8 hours after exercise, whereas providing LC during recovery elicited a sustained/enhanced increase in activation of these genes through 8 to 24 hours of recovery. These findings provide evidence that factors associated with substrate availability and/or cellular metabolic recovery (eg, muscle glycogen restoration) influence the transcriptional regulation of metabolic genes in skeletal muscle of humans during recovery from exercise. SN - 0026-0495 UR - https://www.unboundmedicine.com/medline/citation/16092055/Substrate_availability_and_transcriptional_regulation_of_metabolic_genes_in_human_skeletal_muscle_during_recovery_from_exercise_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0026049505001319 DB - PRIME DP - Unbound Medicine ER -