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Pro-oxidative vs antioxidative properties of ascorbic acid in chromium(VI)-induced damage: an in vivo and in vitro approach.
J Appl Toxicol. 2005 Nov-Dec; 25(6):535-48.JA

Abstract

The effect of antioxidant ascorbic acid (vitamin C) pretreatment on chromium(VI)-induced damage was investigated using the yeast Saccharomyces cerevisiae as a model organism. The objective of this study was to pretreat yeast cells with the antioxidant ascorbic acid in an effort to increase cell tolerance against reactive chromium intermediates and reactive oxygen species formed during chromium(VI) reduction. Intracellular oxidation was estimated using the fluorescence indicators dihidro-2,7-dichlorofluorescein, dihydroethidium and dihydrorhodamine 123. The role of ascorbic acid pretreatment on chromium(VI) toxicity was determined by measuring mitotic gene conversion, reverse mutations, 8-OHdG, hydroxyl radical, superoxide anion and chromium(V) formation. The chromium content in the biomass was determined by flame atomic absorption spectrometry. In the absence of chromium, ascorbic acid effectively protected the cells against endogenous reactive oxygen species formed during normal cellular metabolism. In vitro measurements employing EPR and the results of supercoiled DNA cleavage revealed that the pro-oxidative action of ascorbic acid during Cr(VI) reduction was concentration-dependent and that harmful hydroxyl radical and Cr(V) had formed following Cr(VI) reduction. However, the in vivo results highlighted the important role of increased cytosol reduction capacity related to modification of Cr(V) formation, increased chromium accumulation, better scavenging ability of superoxide anions and hydrogen peroxide, and consequently decreased cytotoxicity and genotoxicity in ascorbic acid pretreated cells. Ascorbic acid influenced Cr(VI) toxicity both as a reducing agent, by decreasing Cr(V) persistence, and as an antioxidant, by decreasing intracellular superoxide anion and hydrogen peroxide formation and by quenching free radicals formed during Cr(VI) to Cr(III) reduction. Increased 8-OHdG and decreased reduced glutathione in ascorbic acid-treated cells might induce an endogenous antioxidant defense system and thus increase cell tolerance against subsequent Cr-induced stress.

Authors+Show Affiliations

University Polytechnic Nova Gorica, School of Environmental Science, Vipavska 13, 5000 Nova Gorica, Slovenia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16092082

Citation

Poljsak, B, et al. "Pro-oxidative Vs Antioxidative Properties of Ascorbic Acid in chromium(VI)-induced Damage: an in Vivo and in Vitro Approach." Journal of Applied Toxicology : JAT, vol. 25, no. 6, 2005, pp. 535-48.
Poljsak B, Gazdag Z, Jenko-Brinovec S, et al. Pro-oxidative vs antioxidative properties of ascorbic acid in chromium(VI)-induced damage: an in vivo and in vitro approach. J Appl Toxicol. 2005;25(6):535-48.
Poljsak, B., Gazdag, Z., Jenko-Brinovec, S., Fujs, S., Pesti, M., Bélagyi, J., Plesnicar, S., & Raspor, P. (2005). Pro-oxidative vs antioxidative properties of ascorbic acid in chromium(VI)-induced damage: an in vivo and in vitro approach. Journal of Applied Toxicology : JAT, 25(6), 535-48.
Poljsak B, et al. Pro-oxidative Vs Antioxidative Properties of Ascorbic Acid in chromium(VI)-induced Damage: an in Vivo and in Vitro Approach. J Appl Toxicol. 2005 Nov-Dec;25(6):535-48. PubMed PMID: 16092082.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pro-oxidative vs antioxidative properties of ascorbic acid in chromium(VI)-induced damage: an in vivo and in vitro approach. AU - Poljsak,B, AU - Gazdag,Z, AU - Jenko-Brinovec,S, AU - Fujs,S, AU - Pesti,M, AU - Bélagyi,J, AU - Plesnicar,S, AU - Raspor,P, PY - 2005/8/11/pubmed PY - 2006/12/9/medline PY - 2005/8/11/entrez SP - 535 EP - 48 JF - Journal of applied toxicology : JAT JO - J Appl Toxicol VL - 25 IS - 6 N2 - The effect of antioxidant ascorbic acid (vitamin C) pretreatment on chromium(VI)-induced damage was investigated using the yeast Saccharomyces cerevisiae as a model organism. The objective of this study was to pretreat yeast cells with the antioxidant ascorbic acid in an effort to increase cell tolerance against reactive chromium intermediates and reactive oxygen species formed during chromium(VI) reduction. Intracellular oxidation was estimated using the fluorescence indicators dihidro-2,7-dichlorofluorescein, dihydroethidium and dihydrorhodamine 123. The role of ascorbic acid pretreatment on chromium(VI) toxicity was determined by measuring mitotic gene conversion, reverse mutations, 8-OHdG, hydroxyl radical, superoxide anion and chromium(V) formation. The chromium content in the biomass was determined by flame atomic absorption spectrometry. In the absence of chromium, ascorbic acid effectively protected the cells against endogenous reactive oxygen species formed during normal cellular metabolism. In vitro measurements employing EPR and the results of supercoiled DNA cleavage revealed that the pro-oxidative action of ascorbic acid during Cr(VI) reduction was concentration-dependent and that harmful hydroxyl radical and Cr(V) had formed following Cr(VI) reduction. However, the in vivo results highlighted the important role of increased cytosol reduction capacity related to modification of Cr(V) formation, increased chromium accumulation, better scavenging ability of superoxide anions and hydrogen peroxide, and consequently decreased cytotoxicity and genotoxicity in ascorbic acid pretreated cells. Ascorbic acid influenced Cr(VI) toxicity both as a reducing agent, by decreasing Cr(V) persistence, and as an antioxidant, by decreasing intracellular superoxide anion and hydrogen peroxide formation and by quenching free radicals formed during Cr(VI) to Cr(III) reduction. Increased 8-OHdG and decreased reduced glutathione in ascorbic acid-treated cells might induce an endogenous antioxidant defense system and thus increase cell tolerance against subsequent Cr-induced stress. SN - 0260-437X UR - https://www.unboundmedicine.com/medline/citation/16092082/Pro_oxidative_vs_antioxidative_properties_of_ascorbic_acid_in_chromium_VI__induced_damage:_an_in_vivo_and_in_vitro_approach_ L2 - https://doi.org/10.1002/jat.1093 DB - PRIME DP - Unbound Medicine ER -