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Competition between R1 and R2 transposable elements in the 28S rRNA genes of insects.
Cytogenet Genome Res. 2005; 110(1-4):299-306.CG

Abstract

R1 and R2 are non-LTR retrotransposons that insert in the 28S rRNA genes of arthropods. R1 elements insert into a site that is 74 bp downstream of the R2 insertion site, thus the presence of an R2 in the same 28S gene may inhibit the expression of R1. Consistent with such a suggestion, the R1 elements of Drosophila melanogaster have a strong bias against inserting into 28S genes already containing an R2 element. R2 elements, on the other hand, are only 2-3 fold inhibited from inserting into a 28S gene already containing an R1. D. melanogaster R1 elements are unusual in that they generate a 23-bp deletion of the target site upstream of the insertion. Using in vitro assays developed to study R2 integration, we show that the presence of R1 sequences 51 bp downstream of the R2 insertion site changes the nucleosomal structure that can be formed by the R2 target site. The R2 endonuclease is inhibited from cleaving these altered nucleosomes. We suggest that R1 elements have been selected to make this large deletion of the 28S gene to block the insertion of an upstream R2 element. These findings are consistent with the model that R1 and R2 are in competition for the limited number of insertion sites available within their host's genome.

Authors+Show Affiliations

Department of Biology, University of Rochester, Rochester, NY 14627, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

16093682

Citation

Ye, J, et al. "Competition Between R1 and R2 Transposable Elements in the 28S rRNA Genes of Insects." Cytogenetic and Genome Research, vol. 110, no. 1-4, 2005, pp. 299-306.
Ye J, Pérez-González CE, Eickbush DG, et al. Competition between R1 and R2 transposable elements in the 28S rRNA genes of insects. Cytogenet Genome Res. 2005;110(1-4):299-306.
Ye, J., Pérez-González, C. E., Eickbush, D. G., & Eickbush, T. H. (2005). Competition between R1 and R2 transposable elements in the 28S rRNA genes of insects. Cytogenetic and Genome Research, 110(1-4), 299-306.
Ye J, et al. Competition Between R1 and R2 Transposable Elements in the 28S rRNA Genes of Insects. Cytogenet Genome Res. 2005;110(1-4):299-306. PubMed PMID: 16093682.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Competition between R1 and R2 transposable elements in the 28S rRNA genes of insects. AU - Ye,J, AU - Pérez-González,C E, AU - Eickbush,D G, AU - Eickbush,T H, PY - 2003/09/16/received PY - 2004/01/13/accepted PY - 2005/8/12/pubmed PY - 2005/10/28/medline PY - 2005/8/12/entrez SP - 299 EP - 306 JF - Cytogenetic and genome research JO - Cytogenet. Genome Res. VL - 110 IS - 1-4 N2 - R1 and R2 are non-LTR retrotransposons that insert in the 28S rRNA genes of arthropods. R1 elements insert into a site that is 74 bp downstream of the R2 insertion site, thus the presence of an R2 in the same 28S gene may inhibit the expression of R1. Consistent with such a suggestion, the R1 elements of Drosophila melanogaster have a strong bias against inserting into 28S genes already containing an R2 element. R2 elements, on the other hand, are only 2-3 fold inhibited from inserting into a 28S gene already containing an R1. D. melanogaster R1 elements are unusual in that they generate a 23-bp deletion of the target site upstream of the insertion. Using in vitro assays developed to study R2 integration, we show that the presence of R1 sequences 51 bp downstream of the R2 insertion site changes the nucleosomal structure that can be formed by the R2 target site. The R2 endonuclease is inhibited from cleaving these altered nucleosomes. We suggest that R1 elements have been selected to make this large deletion of the 28S gene to block the insertion of an upstream R2 element. These findings are consistent with the model that R1 and R2 are in competition for the limited number of insertion sites available within their host's genome. SN - 1424-859X UR - https://www.unboundmedicine.com/medline/citation/16093682/Competition_between_R1_and_R2_transposable_elements_in_the_28S_rRNA_genes_of_insects_ L2 - https://www.karger.com?DOI=10.1159/000084962 DB - PRIME DP - Unbound Medicine ER -