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Plasmid ColE1 DNA replication in Escherichia coli strains temperature-sensitive for DNA replication.
Mol Gen Genet. 1975; 136(4):273-89.MG

Abstract

Mutants of the dnaA, dnaC, dnaD, polC, dnaF and dnaG gene loci were tested for their capacity for colicinogenic plasmid E1 (ColE1) replication at a non-permissive temperature. It was found that ColE1 replication was independent of the dnaA gene function and dependent on dnaC, D, F and G. ColE1 replication in the polC mutant E486 continued for several hours but at a greatly reduced rate. No effect was found of the dnaG mutation on thymine-deprivation-induced "priming" of ColE1 replication at the non-permissive temperature. The mutants also were tested for aberrant replication intermediates of plasmid DNA as well as a temperature sensitive supercoiled DNA-protein relaxation complex. RNA-containing supercoils were found to accumulate in a poIC mutant also blocked for protein synthesis.

Authors+Show Affiliations

Department of Biology, University of California, San Diego, La Jolla, California 92037, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

16094996

Citation

Collins, J, et al. "Plasmid ColE1 DNA Replication in Escherichia Coli Strains Temperature-sensitive for DNA Replication." Molecular & General Genetics : MGG, vol. 136, no. 4, 1975, pp. 273-89.
Collins J, Williams P, Helinski DR. Plasmid ColE1 DNA replication in Escherichia coli strains temperature-sensitive for DNA replication. Mol Gen Genet. 1975;136(4):273-89.
Collins, J., Williams, P., & Helinski, D. R. (1975). Plasmid ColE1 DNA replication in Escherichia coli strains temperature-sensitive for DNA replication. Molecular & General Genetics : MGG, 136(4), 273-89.
Collins J, Williams P, Helinski DR. Plasmid ColE1 DNA Replication in Escherichia Coli Strains Temperature-sensitive for DNA Replication. Mol Gen Genet. 1975;136(4):273-89. PubMed PMID: 16094996.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Plasmid ColE1 DNA replication in Escherichia coli strains temperature-sensitive for DNA replication. AU - Collins,J, AU - Williams,P, AU - Helinski,D R, PY - 1975/1/1/pubmed PY - 2005/9/15/medline PY - 1975/1/1/entrez SP - 273 EP - 89 JF - Molecular & general genetics : MGG JO - Mol Gen Genet VL - 136 IS - 4 N2 - Mutants of the dnaA, dnaC, dnaD, polC, dnaF and dnaG gene loci were tested for their capacity for colicinogenic plasmid E1 (ColE1) replication at a non-permissive temperature. It was found that ColE1 replication was independent of the dnaA gene function and dependent on dnaC, D, F and G. ColE1 replication in the polC mutant E486 continued for several hours but at a greatly reduced rate. No effect was found of the dnaG mutation on thymine-deprivation-induced "priming" of ColE1 replication at the non-permissive temperature. The mutants also were tested for aberrant replication intermediates of plasmid DNA as well as a temperature sensitive supercoiled DNA-protein relaxation complex. RNA-containing supercoils were found to accumulate in a poIC mutant also blocked for protein synthesis. SN - 0026-8925 UR - https://www.unboundmedicine.com/medline/citation/16094996/Plasmid_ColE1_DNA_replication_in_Escherichia_coli_strains_temperature_sensitive_for_DNA_replication_ L2 - https://doi.org/10.1007/BF00341713 DB - PRIME DP - Unbound Medicine ER -