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Immunosuppressive activity of Pollen Typhae ethanol extract on the immune responses in mice.
J Ethnopharmacol. 2005 Dec 01; 102(3):424-9.JE

Abstract

The immunosuppressive activity of the ethanol extract of Pollen Typhae (EEPT) consisting of a mixture of flavonoids, steroids and volatile oils was studied on the immune responses in mice. EEPT significantly suppressed concanavalin A (Con A)- and lipopolysaccharide (LPS)-stimulated splenocyte proliferation in vitro in a concentration-dependent manner. ICR mice were immunized subcutaneously with OVA on days 0 and 14. Beginning on the day of immunization, the mice were administered intraperitoneally with EEPT at a single dose of 0.25, 0.5, and 1.0 mg at intervals of 7 days for a total of five doses. On day 28, antigen-specific antibodies in serum, and mitogen- and OVA-induced splenocyte proliferation were measured. EEPT significantly suppressed Con A-, LPS- and OVA-induced splenocyte proliferation in the OVA-immunized mice in a dose-dependent manner. Moreover, the OVA-specific total IgG, IgG1 and IgG2b levels in the OVA-immunized mice were significantly reduced by EEPT. The results suggest that EEPT could suppress the cellular and humoral response in mice, and deserve further researches to be developed as immunosuppressant.

Authors+Show Affiliations

College of Animal Sciences, Zhejiang University, Hangzhou, China.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16095855

Citation

Qin, Feng, and Hong-Xiang Sun. "Immunosuppressive Activity of Pollen Typhae Ethanol Extract On the Immune Responses in Mice." Journal of Ethnopharmacology, vol. 102, no. 3, 2005, pp. 424-9.
Qin F, Sun HX. Immunosuppressive activity of Pollen Typhae ethanol extract on the immune responses in mice. J Ethnopharmacol. 2005;102(3):424-9.
Qin, F., & Sun, H. X. (2005). Immunosuppressive activity of Pollen Typhae ethanol extract on the immune responses in mice. Journal of Ethnopharmacology, 102(3), 424-9.
Qin F, Sun HX. Immunosuppressive Activity of Pollen Typhae Ethanol Extract On the Immune Responses in Mice. J Ethnopharmacol. 2005 Dec 1;102(3):424-9. PubMed PMID: 16095855.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunosuppressive activity of Pollen Typhae ethanol extract on the immune responses in mice. AU - Qin,Feng, AU - Sun,Hong-Xiang, Y1 - 2005/08/10/ PY - 2005/03/23/received PY - 2005/06/16/revised PY - 2005/06/27/accepted PY - 2005/8/13/pubmed PY - 2006/3/17/medline PY - 2005/8/13/entrez SP - 424 EP - 9 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 102 IS - 3 N2 - The immunosuppressive activity of the ethanol extract of Pollen Typhae (EEPT) consisting of a mixture of flavonoids, steroids and volatile oils was studied on the immune responses in mice. EEPT significantly suppressed concanavalin A (Con A)- and lipopolysaccharide (LPS)-stimulated splenocyte proliferation in vitro in a concentration-dependent manner. ICR mice were immunized subcutaneously with OVA on days 0 and 14. Beginning on the day of immunization, the mice were administered intraperitoneally with EEPT at a single dose of 0.25, 0.5, and 1.0 mg at intervals of 7 days for a total of five doses. On day 28, antigen-specific antibodies in serum, and mitogen- and OVA-induced splenocyte proliferation were measured. EEPT significantly suppressed Con A-, LPS- and OVA-induced splenocyte proliferation in the OVA-immunized mice in a dose-dependent manner. Moreover, the OVA-specific total IgG, IgG1 and IgG2b levels in the OVA-immunized mice were significantly reduced by EEPT. The results suggest that EEPT could suppress the cellular and humoral response in mice, and deserve further researches to be developed as immunosuppressant. SN - 0378-8741 UR - https://www.unboundmedicine.com/medline/citation/16095855/Immunosuppressive_activity_of_Pollen_Typhae_ethanol_extract_on_the_immune_responses_in_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(05)00419-8 DB - PRIME DP - Unbound Medicine ER -