Tags

Type your tag names separated by a space and hit enter

Separation and determination of clotrimazole, methylparaben and propylparaben in pharmaceutical preparation by micellar electrokinetic chromatography.
J Pharm Biomed Anal. 2006 Jan 23; 40(1):215-9.JP

Abstract

In this study, micellar electrokinetic chromatography (MEKC) method was developed for the determination of clotrimazole (CLO), methylparaben (MP) and propylparaben (PP) in a pharmaceutical preparation. Separation was carried out in a fused silica capillary (60 cm x 75 microm i.d.) at 25 kV with UV detection at 212 nm. Optimized background electrolyte (BGE) was 15 mM phosphate buffer (pH 7.2) containing 30 mM sodium dodecyl sulfate (SDS) as a surfactant. Rectilinear calibration ranges were 50-500 mg l(-1) for CLO, 10-100 mg l(-1) for MP and 2.5-25 mg l(-1) for PP. The total analysis time was < 12 min.

Authors+Show Affiliations

Hamoudová R
Charles University, Faculty of Pharmacy, Department of Analytical Chemistry, Heyrovského 1203, CZ-500 05 Hradec Králové, Czech Republic.
Pospísilová M
No affiliation info available
Kavalírová A
No affiliation info available
Solich P
No affiliation info available
Sícha J
No affiliation info available

MeSH

Anti-Infective Agents, LocalCalibrationChemistry Techniques, AnalyticalChemistry, PharmaceuticalChromatography, High Pressure LiquidChromatography, Micellar Electrokinetic CapillaryClotrimazoleElectrolytesHydrogen-Ion ConcentrationModels, ChemicalParabensPhosphatesPreservatives, PharmaceuticalReproducibility of ResultsSensitivity and SpecificitySodium Dodecyl SulfateSurface-Active AgentsTemperatureTime Factors

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16095858

Citation

Hamoudová, Rafífa, et al. "Separation and Determination of Clotrimazole, Methylparaben and Propylparaben in Pharmaceutical Preparation By Micellar Electrokinetic Chromatography." Journal of Pharmaceutical and Biomedical Analysis, vol. 40, no. 1, 2006, pp. 215-9.
Hamoudová R, Pospísilová M, Kavalírová A, et al. Separation and determination of clotrimazole, methylparaben and propylparaben in pharmaceutical preparation by micellar electrokinetic chromatography. J Pharm Biomed Anal. 2006;40(1):215-9.
Hamoudová, R., Pospísilová, M., Kavalírová, A., Solich, P., & Sícha, J. (2006). Separation and determination of clotrimazole, methylparaben and propylparaben in pharmaceutical preparation by micellar electrokinetic chromatography. Journal of Pharmaceutical and Biomedical Analysis, 40(1), 215-9.
Hamoudová R, et al. Separation and Determination of Clotrimazole, Methylparaben and Propylparaben in Pharmaceutical Preparation By Micellar Electrokinetic Chromatography. J Pharm Biomed Anal. 2006 Jan 23;40(1):215-9. PubMed PMID: 16095858.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Separation and determination of clotrimazole, methylparaben and propylparaben in pharmaceutical preparation by micellar electrokinetic chromatography. AU - Hamoudová,Rafífa, AU - Pospísilová,Marie, AU - Kavalírová,Andrea, AU - Solich,Petr, AU - Sícha,Jan, Y1 - 2005/08/10/ PY - 2005/02/06/received PY - 2005/07/03/revised PY - 2005/07/04/accepted PY - 2005/8/13/pubmed PY - 2006/6/7/medline PY - 2005/8/13/entrez SP - 215 EP - 9 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 40 IS - 1 N2 - In this study, micellar electrokinetic chromatography (MEKC) method was developed for the determination of clotrimazole (CLO), methylparaben (MP) and propylparaben (PP) in a pharmaceutical preparation. Separation was carried out in a fused silica capillary (60 cm x 75 microm i.d.) at 25 kV with UV detection at 212 nm. Optimized background electrolyte (BGE) was 15 mM phosphate buffer (pH 7.2) containing 30 mM sodium dodecyl sulfate (SDS) as a surfactant. Rectilinear calibration ranges were 50-500 mg l(-1) for CLO, 10-100 mg l(-1) for MP and 2.5-25 mg l(-1) for PP. The total analysis time was < 12 min. SN - 0731-7085 UR - https://www.unboundmedicine.com/medline/citation/16095858/Separation_and_determination_of_clotrimazole_methylparaben_and_propylparaben_in_pharmaceutical_preparation_by_micellar_electrokinetic_chromatography_ DB - PRIME DP - Unbound Medicine ER -