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Analysis of protein interactions on protein arrays by a novel spectral surface plasmon resonance imaging.
Biosens Bioelectron. 2006 Feb 15; 21(8):1521-8.BB

Abstract

We presented a novel surface plasmon resonance (SPR) imaging method for analysis of protein arrays based on a wavelength interrogation-based SPR biosensor. The spectral imaging was performed by the combination of position control and resonance wavelengths calculated from SPR reflectivity spectra. The imaging method was evaluated by analyzing interactions of glutathione S-transferase-fusion proteins with their antibodies. Antigen-antibody interactions were successfully analyzed on glutathione S-transferase-fusion protein arrays by using the spectral imaging method, and the results were confirmed by a parallel analysis using a previously used spectral SPR biosensor based on wavelength interrogation. Specific binding of anti-Rac1 and anti-RhoA to Rac1 and RhoA on the protein arrays was qualitatively and quantitatively analyzed by the spectral SPR imaging. Thus, it was suggested that the novel spectral SPR imaging was a useful tool for the high-throughput analysis of protein-protein interactions on protein arrays.

Authors+Show Affiliations

Yuk JS
Department of Molecular and Cellular Biochemistry and Nano-Bio Sensor Research Center, Kangwon National University College of Medicine, Chunchon, South Korea.
Kim HS
No affiliation info available
Jung JW
No affiliation info available
Jung SH
No affiliation info available
Lee SJ
No affiliation info available
Kim WJ
No affiliation info available
Han JA
No affiliation info available
Kim YM
No affiliation info available
Ha KS
No affiliation info available

MeSH

Antigen-Antibody ComplexBiosensing TechniquesEquipment DesignEquipment Failure AnalysisImmunoassayProtein Array AnalysisProtein Interaction MappingReproducibility of ResultsSensitivity and SpecificitySurface Plasmon Resonance

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16095894

Citation

Yuk, Jong Seol, et al. "Analysis of Protein Interactions On Protein Arrays By a Novel Spectral Surface Plasmon Resonance Imaging." Biosensors & Bioelectronics, vol. 21, no. 8, 2006, pp. 1521-8.
Yuk JS, Kim HS, Jung JW, et al. Analysis of protein interactions on protein arrays by a novel spectral surface plasmon resonance imaging. Biosens Bioelectron. 2006;21(8):1521-8.
Yuk, J. S., Kim, H. S., Jung, J. W., Jung, S. H., Lee, S. J., Kim, W. J., Han, J. A., Kim, Y. M., & Ha, K. S. (2006). Analysis of protein interactions on protein arrays by a novel spectral surface plasmon resonance imaging. Biosensors & Bioelectronics, 21(8), 1521-8.
Yuk JS, et al. Analysis of Protein Interactions On Protein Arrays By a Novel Spectral Surface Plasmon Resonance Imaging. Biosens Bioelectron. 2006 Feb 15;21(8):1521-8. PubMed PMID: 16095894.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of protein interactions on protein arrays by a novel spectral surface plasmon resonance imaging. AU - Yuk,Jong Seol, AU - Kim,Hyun-Soo, AU - Jung,Jae-Wan, AU - Jung,Se-Hui, AU - Lee,Seung-Joon, AU - Kim,Woo Jin, AU - Han,Jeong-A, AU - Kim,Young-Myeong, AU - Ha,Kwon-Soo, Y1 - 2005/08/10/ PY - 2005/04/13/received PY - 2005/06/28/revised PY - 2005/07/06/accepted PY - 2005/8/13/pubmed PY - 2006/4/14/medline PY - 2005/8/13/entrez SP - 1521 EP - 8 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 21 IS - 8 N2 - We presented a novel surface plasmon resonance (SPR) imaging method for analysis of protein arrays based on a wavelength interrogation-based SPR biosensor. The spectral imaging was performed by the combination of position control and resonance wavelengths calculated from SPR reflectivity spectra. The imaging method was evaluated by analyzing interactions of glutathione S-transferase-fusion proteins with their antibodies. Antigen-antibody interactions were successfully analyzed on glutathione S-transferase-fusion protein arrays by using the spectral imaging method, and the results were confirmed by a parallel analysis using a previously used spectral SPR biosensor based on wavelength interrogation. Specific binding of anti-Rac1 and anti-RhoA to Rac1 and RhoA on the protein arrays was qualitatively and quantitatively analyzed by the spectral SPR imaging. Thus, it was suggested that the novel spectral SPR imaging was a useful tool for the high-throughput analysis of protein-protein interactions on protein arrays. SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/16095894/Analysis_of_protein_interactions_on_protein_arrays_by_a_novel_spectral_surface_plasmon_resonance_imaging_ DB - PRIME DP - Unbound Medicine ER -