Tags

Type your tag names separated by a space and hit enter

Determination of histamine in wines with an on-line pre-column flow derivatization system coupled to high performance liquid chromatography.
Analyst. 2005 Sep; 130(9):1286-90.A

Abstract

A new rapid and sensitive high performance liquid chromatography (HPLC) method for determining histamine in red wine samples, based on continuous flow derivatization with 1,2-naphthoquinone-4-sulfonate (NQS), is proposed. In this system, samples are derivatized on-line in a three-channel flow manifold for reagent, buffer and sample. The reaction takes place in a PTFE coil heated at 80 degrees C and with a residence time of 2.9 min. The reaction mixture is injected directly into the chromatographic system, where the histamine derivative is separated from other aminated compounds present in the wine matrix in less than ten minutes. The HPLC procedure involves a C18 column, a binary gradient of 2% acetic acid-methanol as a mobile phase, and UV detection at 305 nm. Analytical parameters of the method are evaluated using red wine samples. The linear range is up to 66.7 mg L(-1) (r = 0.9999), the precision (RSD) is 3%, the detection limit is 0.22 mg L(-1), and the average histamine recovery is 101.5% +/- 6.7%. Commercial red wines from different Spanish regions are analyzed with the proposed method.

Authors+Show Affiliations

Department of Analytical Chemistry. University of Barcelona, Diagonal 647, 08028-Barcelona, Spain. nati@apolo.qui.ub.esNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16096675

Citation

García-Villar, Natividad, et al. "Determination of Histamine in Wines With an On-line Pre-column Flow Derivatization System Coupled to High Performance Liquid Chromatography." The Analyst, vol. 130, no. 9, 2005, pp. 1286-90.
García-Villar N, Saurina J, Hernández-Cassou S. Determination of histamine in wines with an on-line pre-column flow derivatization system coupled to high performance liquid chromatography. Analyst. 2005;130(9):1286-90.
García-Villar, N., Saurina, J., & Hernández-Cassou, S. (2005). Determination of histamine in wines with an on-line pre-column flow derivatization system coupled to high performance liquid chromatography. The Analyst, 130(9), 1286-90.
García-Villar N, Saurina J, Hernández-Cassou S. Determination of Histamine in Wines With an On-line Pre-column Flow Derivatization System Coupled to High Performance Liquid Chromatography. Analyst. 2005;130(9):1286-90. PubMed PMID: 16096675.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of histamine in wines with an on-line pre-column flow derivatization system coupled to high performance liquid chromatography. AU - García-Villar,Natividad, AU - Saurina,Javier, AU - Hernández-Cassou,Santiago, Y1 - 2005/07/28/ PY - 2005/8/13/pubmed PY - 2005/11/15/medline PY - 2005/8/13/entrez SP - 1286 EP - 90 JF - The Analyst JO - Analyst VL - 130 IS - 9 N2 - A new rapid and sensitive high performance liquid chromatography (HPLC) method for determining histamine in red wine samples, based on continuous flow derivatization with 1,2-naphthoquinone-4-sulfonate (NQS), is proposed. In this system, samples are derivatized on-line in a three-channel flow manifold for reagent, buffer and sample. The reaction takes place in a PTFE coil heated at 80 degrees C and with a residence time of 2.9 min. The reaction mixture is injected directly into the chromatographic system, where the histamine derivative is separated from other aminated compounds present in the wine matrix in less than ten minutes. The HPLC procedure involves a C18 column, a binary gradient of 2% acetic acid-methanol as a mobile phase, and UV detection at 305 nm. Analytical parameters of the method are evaluated using red wine samples. The linear range is up to 66.7 mg L(-1) (r = 0.9999), the precision (RSD) is 3%, the detection limit is 0.22 mg L(-1), and the average histamine recovery is 101.5% +/- 6.7%. Commercial red wines from different Spanish regions are analyzed with the proposed method. SN - 0003-2654 UR - https://www.unboundmedicine.com/medline/citation/16096675/Determination_of_histamine_in_wines_with_an_on_line_pre_column_flow_derivatization_system_coupled_to_high_performance_liquid_chromatography_ DB - PRIME DP - Unbound Medicine ER -