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Limited effects of combined dietary copper deficiency/iron overload on oxidative stress parameters in rat liver and plasma.

Abstract

Copper (Cu) deficiency decreases the activity of Cu-dependent antioxidant enzymes such as Cu,zinc-superoxide dismutase (Cu,Zn-SOD) and may be associated with increased susceptibility to oxidative stress. Iron (Fe) overload represents a dietary oxidative stress relevant to overuse of Fe-containing supplements and to hereditary hemochromatosis. In a study to investigate oxidative stress interactions of dietary Cu deficiency with Fe overload, weanling male Long-Evans rats were fed one of four sucrose-based modified AIN-93G diets formulated to differ in Cu (adequate 6 mg/kg diet vs. deficient 0.5 mg/kg) and Fe (adequate 35 mg/kg vs. overloaded 1500 mg/kg) in a 2 x 2 factorial design for 4 weeks prior to necropsy. Care was taken to minimize oxidation of the diets prior to feeding to the rats. Liver and plasma Cu content and liver Cu,Zn-SOD activity declined with Cu deficiency and liver Fe increased with Fe overload, confirming the experimental dietary model. Liver thiobarbituric acid reactive substances were significantly elevated with Fe overload (pooled across Cu treatments, 0.80+/-0.14 vs. 0.54+/-0.08 nmol/mg protein; P<.0001) and not affected by Cu deficiency. Liver cytosolic protein carbonyl content and the concentrations of several oxidized cholesterol species in liver tissue did not change with these dietary treatments. Plasma protein carbonyl content decreased in Cu-deficient rats and was not influenced by dietary Fe overload. The various substrates (lipid, protein and cholesterol) appeared to differ in their susceptibility to the in vivo oxidative stress induced by dietary Fe overload, but these differences were not exacerbated by Cu deficiency.

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  • Authors+Show Affiliations

    ,

    Nutrition Research Division, Health Products and Food Branch, Health Canada, Ottawa, ON, Canada K1A 0L2. kevin_cockell@hc-sc.gc.ca

    , , , , , , ,

    Source

    MeSH

    Animals
    Copper
    Diet
    Iron
    Iron Overload
    Liver
    Male
    Oxidative Stress
    Protein Carbonylation
    Rats
    Rats, Long-Evans
    Superoxide Dismutase
    Thiobarbituric Acid Reactive Substances

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    16098731

    Citation

    Cockell, Kevin A., et al. "Limited Effects of Combined Dietary Copper Deficiency/iron Overload On Oxidative Stress Parameters in Rat Liver and Plasma." The Journal of Nutritional Biochemistry, vol. 16, no. 12, 2005, pp. 750-6.
    Cockell KA, Wotherspoon AT, Belonje B, et al. Limited effects of combined dietary copper deficiency/iron overload on oxidative stress parameters in rat liver and plasma. J Nutr Biochem. 2005;16(12):750-6.
    Cockell, K. A., Wotherspoon, A. T., Belonje, B., Fritz, M. E., Madère, R., Hidiroglou, N., ... Kubow, S. (2005). Limited effects of combined dietary copper deficiency/iron overload on oxidative stress parameters in rat liver and plasma. The Journal of Nutritional Biochemistry, 16(12), pp. 750-6.
    Cockell KA, et al. Limited Effects of Combined Dietary Copper Deficiency/iron Overload On Oxidative Stress Parameters in Rat Liver and Plasma. J Nutr Biochem. 2005;16(12):750-6. PubMed PMID: 16098731.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Limited effects of combined dietary copper deficiency/iron overload on oxidative stress parameters in rat liver and plasma. AU - Cockell,Kevin A, AU - Wotherspoon,Andrew T L, AU - Belonje,Bartholomeus, AU - Fritz,Melissa E, AU - Madère,René, AU - Hidiroglou,Nick, AU - Plouffe,Louise J, AU - Ratnayake,W M Nimal, AU - Kubow,Stan, Y1 - 2005/08/10/ PY - 2005/01/21/received PY - 2005/04/06/revised PY - 2005/04/18/accepted PY - 2005/8/16/pubmed PY - 2006/1/7/medline PY - 2005/8/16/entrez SP - 750 EP - 6 JF - The Journal of nutritional biochemistry JO - J. Nutr. Biochem. VL - 16 IS - 12 N2 - Copper (Cu) deficiency decreases the activity of Cu-dependent antioxidant enzymes such as Cu,zinc-superoxide dismutase (Cu,Zn-SOD) and may be associated with increased susceptibility to oxidative stress. Iron (Fe) overload represents a dietary oxidative stress relevant to overuse of Fe-containing supplements and to hereditary hemochromatosis. In a study to investigate oxidative stress interactions of dietary Cu deficiency with Fe overload, weanling male Long-Evans rats were fed one of four sucrose-based modified AIN-93G diets formulated to differ in Cu (adequate 6 mg/kg diet vs. deficient 0.5 mg/kg) and Fe (adequate 35 mg/kg vs. overloaded 1500 mg/kg) in a 2 x 2 factorial design for 4 weeks prior to necropsy. Care was taken to minimize oxidation of the diets prior to feeding to the rats. Liver and plasma Cu content and liver Cu,Zn-SOD activity declined with Cu deficiency and liver Fe increased with Fe overload, confirming the experimental dietary model. Liver thiobarbituric acid reactive substances were significantly elevated with Fe overload (pooled across Cu treatments, 0.80+/-0.14 vs. 0.54+/-0.08 nmol/mg protein; P<.0001) and not affected by Cu deficiency. Liver cytosolic protein carbonyl content and the concentrations of several oxidized cholesterol species in liver tissue did not change with these dietary treatments. Plasma protein carbonyl content decreased in Cu-deficient rats and was not influenced by dietary Fe overload. The various substrates (lipid, protein and cholesterol) appeared to differ in their susceptibility to the in vivo oxidative stress induced by dietary Fe overload, but these differences were not exacerbated by Cu deficiency. SN - 0955-2863 UR - https://www.unboundmedicine.com/medline/citation/16098731/Limited_effects_of_combined_dietary_copper_deficiency/iron_overload_on_oxidative_stress_parameters_in_rat_liver_and_plasma_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0955-2863(05)00117-8 DB - PRIME DP - Unbound Medicine ER -