Tags

Type your tag names separated by a space and hit enter

Anti-diabetic action of Punica granatum flower extract: activation of PPAR-gamma and identification of an active component.
Toxicol Appl Pharmacol. 2005 Sep 01; 207(2):160-9.TA

Abstract

Peroxisome proliferator-activated receptor (PPAR)-gamma activators are widely used in the treatment of type 2 diabetes because they improve the sensitivity of insulin receptors. Punica granatum flower (PGF) has been used as an anti-diabetic medicine in Unani medicinal literature. The mechanism of actions is, however, unknown. In the current study, we demonstrated that 6-week oral administration of methanol extract from PGF (500 mg/kg, daily) inhibited glucose loading-induced increase of plasma glucose levels in Zucker diabetic fatty rats (ZDF), a genetic animal model for type 2 diabetes, whereas it did not inhibit the increase in Zucker lean rats (ZL). The treatment did not lower the plasma glucose levels in fasted ZDF and ZL rats. Furthermore, RT-PCR results demonstrated that the PGF extract treatment in ZDF rats enhanced cardiac PPAR-gamma mRNA expression and restored the down-regulated cardiac glucose transporter (GLUT)-4 (the insulin-dependent isoform of GLUTs) mRNA. These results suggest that the anti-diabetic activity of PGF extract may result from improved sensitivity of the insulin receptor. From the in vitro studies, we demonstrated that the PGF extract enhanced PPAR-gamma mRNA and protein expression and increased PPAR-gamma-dependent mRNA expression and activity of lipoprotein lipase in human THP-1-differentiated macrophage cells. Phytochemical investigation demonstrated that gallic acid in PGF extract is mostly responsible for this activity. Thus, our findings indicate that PPAR-gamma is a molecular target for PGF extract and its prominent component gallic acid, and provide a better understanding of the potential mechanism of the anti-diabetic action of PGF.

Authors+Show Affiliations

Herbal Medicines Research and Education Centre, The University of Sydney, NSW 2006 Australia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16102567

Citation

Huang, Tom H W., et al. "Anti-diabetic Action of Punica Granatum Flower Extract: Activation of PPAR-gamma and Identification of an Active Component." Toxicology and Applied Pharmacology, vol. 207, no. 2, 2005, pp. 160-9.
Huang TH, Peng G, Kota BP, et al. Anti-diabetic action of Punica granatum flower extract: activation of PPAR-gamma and identification of an active component. Toxicol Appl Pharmacol. 2005;207(2):160-9.
Huang, T. H., Peng, G., Kota, B. P., Li, G. Q., Yamahara, J., Roufogalis, B. D., & Li, Y. (2005). Anti-diabetic action of Punica granatum flower extract: activation of PPAR-gamma and identification of an active component. Toxicology and Applied Pharmacology, 207(2), 160-9.
Huang TH, et al. Anti-diabetic Action of Punica Granatum Flower Extract: Activation of PPAR-gamma and Identification of an Active Component. Toxicol Appl Pharmacol. 2005 Sep 1;207(2):160-9. PubMed PMID: 16102567.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-diabetic action of Punica granatum flower extract: activation of PPAR-gamma and identification of an active component. AU - Huang,Tom H W, AU - Peng,Gang, AU - Kota,Bhavani P, AU - Li,George Q, AU - Yamahara,Johji, AU - Roufogalis,Basil D, AU - Li,Yuhao, PY - 2004/10/01/received PY - 2004/11/25/revised PY - 2004/12/16/accepted PY - 2005/8/17/pubmed PY - 2005/9/30/medline PY - 2005/8/17/entrez SP - 160 EP - 9 JF - Toxicology and applied pharmacology JO - Toxicol. Appl. Pharmacol. VL - 207 IS - 2 N2 - Peroxisome proliferator-activated receptor (PPAR)-gamma activators are widely used in the treatment of type 2 diabetes because they improve the sensitivity of insulin receptors. Punica granatum flower (PGF) has been used as an anti-diabetic medicine in Unani medicinal literature. The mechanism of actions is, however, unknown. In the current study, we demonstrated that 6-week oral administration of methanol extract from PGF (500 mg/kg, daily) inhibited glucose loading-induced increase of plasma glucose levels in Zucker diabetic fatty rats (ZDF), a genetic animal model for type 2 diabetes, whereas it did not inhibit the increase in Zucker lean rats (ZL). The treatment did not lower the plasma glucose levels in fasted ZDF and ZL rats. Furthermore, RT-PCR results demonstrated that the PGF extract treatment in ZDF rats enhanced cardiac PPAR-gamma mRNA expression and restored the down-regulated cardiac glucose transporter (GLUT)-4 (the insulin-dependent isoform of GLUTs) mRNA. These results suggest that the anti-diabetic activity of PGF extract may result from improved sensitivity of the insulin receptor. From the in vitro studies, we demonstrated that the PGF extract enhanced PPAR-gamma mRNA and protein expression and increased PPAR-gamma-dependent mRNA expression and activity of lipoprotein lipase in human THP-1-differentiated macrophage cells. Phytochemical investigation demonstrated that gallic acid in PGF extract is mostly responsible for this activity. Thus, our findings indicate that PPAR-gamma is a molecular target for PGF extract and its prominent component gallic acid, and provide a better understanding of the potential mechanism of the anti-diabetic action of PGF. SN - 0041-008X UR - https://www.unboundmedicine.com/medline/citation/16102567/Anti_diabetic_action_of_Punica_granatum_flower_extract:_activation_of_PPAR_gamma_and_identification_of_an_active_component_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041-008X(04)00576-9 DB - PRIME DP - Unbound Medicine ER -