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The polypyrimidine tract-binding protein (PTB) is required for efficient replication of hepatitis C virus (HCV) RNA.
Virus Res. 2006 Jan; 115(1):1-8.VR

Abstract

Our earlier work found that the polypyrimidine tract-binding protein (PTB) specifically interacts with the poly(U/C) tract of the hepatitis C virus (HCV) 3' untranslated region (3'UTR) [Luo, G., 1999. Cellular proteins bind to the poly(U) tract of the 3' untranslated region of hepatitis C virus RNA genome. Virology 256, 105-118]. We report here that the phosphorylated form of PTB is associated with the membrane-bound HCV replication complex. To determine whether the PTB is required for HCV RNA replication, synthetic small interfering RNAs (siRNAs) were used to specifically knockdown PTB expression in the HCV replicon-harboring Huh7 cells. The level of PTB expression was efficiently reduced by PTB-specific siRNAs. Consequently, the levels of HCV proteins as well as HCV RNA were proportionally decreased by increasing concentrations of PTB siRNA. However, the translation mediated by the encephamyocarditis virus internal ribosome entry site was unaffected, suggesting that the inhibition of HCV RNA replication by PTB siRNA was not due to its effect on the EMCV IRES-mediated translation. Collectively, these findings demonstrate that PTB is required for efficient HCV RNA replication in the cell.

Authors+Show Affiliations

Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine, 800 Rose Street, Lexington, 40536-0298, USA.No affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

16102869

Citation

Chang, Kyung-Soo, and Guangxiang Luo. "The Polypyrimidine Tract-binding Protein (PTB) Is Required for Efficient Replication of Hepatitis C Virus (HCV) RNA." Virus Research, vol. 115, no. 1, 2006, pp. 1-8.
Chang KS, Luo G. The polypyrimidine tract-binding protein (PTB) is required for efficient replication of hepatitis C virus (HCV) RNA. Virus Res. 2006;115(1):1-8.
Chang, K. S., & Luo, G. (2006). The polypyrimidine tract-binding protein (PTB) is required for efficient replication of hepatitis C virus (HCV) RNA. Virus Research, 115(1), 1-8.
Chang KS, Luo G. The Polypyrimidine Tract-binding Protein (PTB) Is Required for Efficient Replication of Hepatitis C Virus (HCV) RNA. Virus Res. 2006;115(1):1-8. PubMed PMID: 16102869.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The polypyrimidine tract-binding protein (PTB) is required for efficient replication of hepatitis C virus (HCV) RNA. AU - Chang,Kyung-Soo, AU - Luo,Guangxiang, Y1 - 2005/08/15/ PY - 2005/04/13/received PY - 2005/06/10/revised PY - 2005/06/27/accepted PY - 2005/8/17/pubmed PY - 2006/3/17/medline PY - 2005/8/17/entrez SP - 1 EP - 8 JF - Virus research JO - Virus Res VL - 115 IS - 1 N2 - Our earlier work found that the polypyrimidine tract-binding protein (PTB) specifically interacts with the poly(U/C) tract of the hepatitis C virus (HCV) 3' untranslated region (3'UTR) [Luo, G., 1999. Cellular proteins bind to the poly(U) tract of the 3' untranslated region of hepatitis C virus RNA genome. Virology 256, 105-118]. We report here that the phosphorylated form of PTB is associated with the membrane-bound HCV replication complex. To determine whether the PTB is required for HCV RNA replication, synthetic small interfering RNAs (siRNAs) were used to specifically knockdown PTB expression in the HCV replicon-harboring Huh7 cells. The level of PTB expression was efficiently reduced by PTB-specific siRNAs. Consequently, the levels of HCV proteins as well as HCV RNA were proportionally decreased by increasing concentrations of PTB siRNA. However, the translation mediated by the encephamyocarditis virus internal ribosome entry site was unaffected, suggesting that the inhibition of HCV RNA replication by PTB siRNA was not due to its effect on the EMCV IRES-mediated translation. Collectively, these findings demonstrate that PTB is required for efficient HCV RNA replication in the cell. SN - 0168-1702 UR - https://www.unboundmedicine.com/medline/citation/16102869/The_polypyrimidine_tract_binding_protein__PTB__is_required_for_efficient_replication_of_hepatitis_C_virus__HCV__RNA_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-1702(05)00218-2 DB - PRIME DP - Unbound Medicine ER -