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Sex differences in cytokine production and surface antigen expression of peripheral blood mononuclear cells after surgery.
Am J Surg. 2005 Sep; 190(3):439-44.AJ

Abstract

BACKGROUND

Several clinical and epidemiological studies have observed a better outcome after sepsis in women than in men. The purpose of this study was to determine if these sex differences are observed in cytokine responses and the surface antigen expression of monocytes. In addition, the clinical courses of male and female patients after gastrointestinal surgery were compared.

METHODS

A total of 25 patients with gastric carcinoma who underwent gastrectomy were enrolled in this study. Tumor necrosis factor-alpha (TNF-alpha), interleukin-10, and interferon-gamma (IFN-gamma) production by lipopolysaccharide-stimulated peripheral blood mononuclear cells (PBMCs) as well as the expression of Toll-like receptor-4 (TLR-4), TLR-2, human leukocyte antigen-D related (HLA-DR), and CD16 on monocytes in 16 men and 9 women on the day before surgery were compared with measurements on postoperative day (POD) 1. Furthermore, postoperative infectious complications, the development of systemic inflammatory response syndrome, and serum C-reactive protein levels on POD3 were compared.

RESULTS

TNF-alpha production of PBMCs and TLR-2 and CD16 expression on monocytes were significantly higher in women than in men before surgery. IFN-gamma production of PBMCs and HLA-DR expression on monocytes were significantly lower in men than in women on POD1. Furthermore, TNF-alpha production of PBMCs on POD1 was significantly increased, and both IFN-gamma production and HLA-DR expression were significantly decreased compared with that observed before surgery in men, but no corresponding significant changes were observed in women. In addition, C-reactive protein levels on POD3 were significantly higher in men than in women.

CONCLUSIONS

Both TNF-alpha and interleukin-10 production of PBMCs and both TLR-2 and CD16 expression on monocytes were significantly higher in women than in men on the day before surgery. Excessive TNF-alpha and suppressive IFN-gamma production of PBMCs, as well as a decrease in HLA-DR expression on monocytes, occurred more often in men than in women after surgery, suggesting that these factors all contribute to an increased susceptibility of men to develop systemic inflammatory response syndrome or postoperative infectious complications.

Authors+Show Affiliations

Department of Surgery I, National Defense Medical College Research Institute, Namiki 3-2, Tokorozawa, Saitama, 359-8513, Japan. satoshi@ndmc.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

16105533

Citation

Ono, Satoshi, et al. "Sex Differences in Cytokine Production and Surface Antigen Expression of Peripheral Blood Mononuclear Cells After Surgery." American Journal of Surgery, vol. 190, no. 3, 2005, pp. 439-44.
Ono S, Tsujimoto H, Hiraki S, et al. Sex differences in cytokine production and surface antigen expression of peripheral blood mononuclear cells after surgery. Am J Surg. 2005;190(3):439-44.
Ono, S., Tsujimoto, H., Hiraki, S., Takahata, R., Kinoshita, M., & Mochizuki, H. (2005). Sex differences in cytokine production and surface antigen expression of peripheral blood mononuclear cells after surgery. American Journal of Surgery, 190(3), 439-44.
Ono S, et al. Sex Differences in Cytokine Production and Surface Antigen Expression of Peripheral Blood Mononuclear Cells After Surgery. Am J Surg. 2005;190(3):439-44. PubMed PMID: 16105533.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sex differences in cytokine production and surface antigen expression of peripheral blood mononuclear cells after surgery. AU - Ono,Satoshi, AU - Tsujimoto,Hironori, AU - Hiraki,Shu-Ichi, AU - Takahata,Risa, AU - Kinoshita,Manabu, AU - Mochizuki,Hidetaka, PY - 2004/09/01/received PY - 2005/03/25/revised PY - 2005/03/25/accepted PY - 2005/8/18/pubmed PY - 2005/9/28/medline PY - 2005/8/18/entrez SP - 439 EP - 44 JF - American journal of surgery JO - Am J Surg VL - 190 IS - 3 N2 - BACKGROUND: Several clinical and epidemiological studies have observed a better outcome after sepsis in women than in men. The purpose of this study was to determine if these sex differences are observed in cytokine responses and the surface antigen expression of monocytes. In addition, the clinical courses of male and female patients after gastrointestinal surgery were compared. METHODS: A total of 25 patients with gastric carcinoma who underwent gastrectomy were enrolled in this study. Tumor necrosis factor-alpha (TNF-alpha), interleukin-10, and interferon-gamma (IFN-gamma) production by lipopolysaccharide-stimulated peripheral blood mononuclear cells (PBMCs) as well as the expression of Toll-like receptor-4 (TLR-4), TLR-2, human leukocyte antigen-D related (HLA-DR), and CD16 on monocytes in 16 men and 9 women on the day before surgery were compared with measurements on postoperative day (POD) 1. Furthermore, postoperative infectious complications, the development of systemic inflammatory response syndrome, and serum C-reactive protein levels on POD3 were compared. RESULTS: TNF-alpha production of PBMCs and TLR-2 and CD16 expression on monocytes were significantly higher in women than in men before surgery. IFN-gamma production of PBMCs and HLA-DR expression on monocytes were significantly lower in men than in women on POD1. Furthermore, TNF-alpha production of PBMCs on POD1 was significantly increased, and both IFN-gamma production and HLA-DR expression were significantly decreased compared with that observed before surgery in men, but no corresponding significant changes were observed in women. In addition, C-reactive protein levels on POD3 were significantly higher in men than in women. CONCLUSIONS: Both TNF-alpha and interleukin-10 production of PBMCs and both TLR-2 and CD16 expression on monocytes were significantly higher in women than in men on the day before surgery. Excessive TNF-alpha and suppressive IFN-gamma production of PBMCs, as well as a decrease in HLA-DR expression on monocytes, occurred more often in men than in women after surgery, suggesting that these factors all contribute to an increased susceptibility of men to develop systemic inflammatory response syndrome or postoperative infectious complications. SN - 0002-9610 UR - https://www.unboundmedicine.com/medline/citation/16105533/Sex_differences_in_cytokine_production_and_surface_antigen_expression_of_peripheral_blood_mononuclear_cells_after_surgery_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0002-9610(05)00496-4 DB - PRIME DP - Unbound Medicine ER -