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Transgenic expression of Cre recombinase in mitral/tufted cells of the olfactory bulb.
Genesis. 2005 Sep; 43(1):12-6.G

Abstract

Olfactory information is conveyed from the periphery to the olfactory cortices through mitral and tufted (M/T) cells in the olfactory bulb. A mouse with a specific expression of Cre recombinase in M/T cells is essential for genetic marking of M/T cells and manipulating their properties. Protocadherin 21 (Pcdh21) expression is highly restricted to M/T cells. Here we report a transgenic mouse line, Pcdh21-Cre, in which 10-kb mouse Pcdh21 promoter drives the expression of Cre recombinase. In Pcdh21-Cre mice, Cre recombinase activity is predominantly detected in M/T cells, visualized with the anti-CFP immunostaining in offspring of a cross between Pcdh21-Cre and the reporter Rosa26-loxP-stop-loxP-CFP strain. These results demonstrate that the 10-kb Pcdh21 promoter can drive transcription in M/T cells and Pcdh21-Cre mice can be used to excise floxed DNA fragments in M/T cells, which provides a valuable tool to reveal the structure and function of the central olfactory circuits.

Authors+Show Affiliations

Molecular Neurogenetics Unit, HMRO, Kyoto University Graduate School of Medicine, Yoshida-Konoe, Sakyo, Kyoto, Japan.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16106355

Citation

Nagai, Yumiko, et al. "Transgenic Expression of Cre Recombinase in Mitral/tufted Cells of the Olfactory Bulb." Genesis (New York, N.Y. : 2000), vol. 43, no. 1, 2005, pp. 12-6.
Nagai Y, Sano H, Yokoi M. Transgenic expression of Cre recombinase in mitral/tufted cells of the olfactory bulb. Genesis. 2005;43(1):12-6.
Nagai, Y., Sano, H., & Yokoi, M. (2005). Transgenic expression of Cre recombinase in mitral/tufted cells of the olfactory bulb. Genesis (New York, N.Y. : 2000), 43(1), 12-6.
Nagai Y, Sano H, Yokoi M. Transgenic Expression of Cre Recombinase in Mitral/tufted Cells of the Olfactory Bulb. Genesis. 2005;43(1):12-6. PubMed PMID: 16106355.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transgenic expression of Cre recombinase in mitral/tufted cells of the olfactory bulb. AU - Nagai,Yumiko, AU - Sano,Hiromi, AU - Yokoi,Mineto, PY - 2005/8/18/pubmed PY - 2008/3/26/medline PY - 2005/8/18/entrez SP - 12 EP - 6 JF - Genesis (New York, N.Y. : 2000) JO - Genesis VL - 43 IS - 1 N2 - Olfactory information is conveyed from the periphery to the olfactory cortices through mitral and tufted (M/T) cells in the olfactory bulb. A mouse with a specific expression of Cre recombinase in M/T cells is essential for genetic marking of M/T cells and manipulating their properties. Protocadherin 21 (Pcdh21) expression is highly restricted to M/T cells. Here we report a transgenic mouse line, Pcdh21-Cre, in which 10-kb mouse Pcdh21 promoter drives the expression of Cre recombinase. In Pcdh21-Cre mice, Cre recombinase activity is predominantly detected in M/T cells, visualized with the anti-CFP immunostaining in offspring of a cross between Pcdh21-Cre and the reporter Rosa26-loxP-stop-loxP-CFP strain. These results demonstrate that the 10-kb Pcdh21 promoter can drive transcription in M/T cells and Pcdh21-Cre mice can be used to excise floxed DNA fragments in M/T cells, which provides a valuable tool to reveal the structure and function of the central olfactory circuits. SN - 1526-954X UR - https://www.unboundmedicine.com/medline/citation/16106355/Transgenic_expression_of_Cre_recombinase_in_mitral/tufted_cells_of_the_olfactory_bulb_ L2 - https://doi.org/10.1002/gene.20146 DB - PRIME DP - Unbound Medicine ER -