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mRNA expression of antioxidant enzymes (SOD, CAT and GSH-Px) and lipid peroxidative stress in liver of Atlantic salmon (Salmo salar) exposed to hyperoxic water during smoltification.
Comp Biochem Physiol C Toxicol Pharmacol. 2005 Jul; 141(3):314-23.CB

Abstract

The mRNA levels of three antioxidant genes, Cu/Zn superoxide dismutase (SOD), catalase (CAT) and phospholipid hydroperoxide glutathione peroxidase (GSH-Px), were quantified with real-time qRT-PCR in liver of Atlantic salmon Salmo salar exposed to 80% (normoxia), 105% and 130% O2 saturation for 54 days. The salmon were then translocated and exposed to 90% and 130% O2 saturation for additional 72 days during smoltification. TBARS and vitamin E levels in liver and the levels of oxidized glutathione (GSSG), total glutathione (GSH) and the resulting oxidative stress index (OSI) in blood were quantified as traditional oxidative stress markers. No significant mean normalized expression (MNE) differences of SOD, CAT or GSH-Px were found in liver after hyperoxia exposure at the two sampling times. Significantly decreased OSI was found in smolt exposed to 130% O2 saturation after 126 days (n = 18, P < 0.0001), indicating hyperoxia-induced oxidative stress. No effects were seen on growth, or on the levels of thiobarbituric reactive substances (TBARS) and vitamin E in liver after the exposure experiment. Overall, the mRNA expression of SOD, CAT and GSH-Px in liver related poorly with the hyperoxic exposure regimes, and more knowledge are needed before the expressed levels of these antioxidant genes can be applied as biomarkers of hyperoxia in Atlantic salmon.

Authors+Show Affiliations

National Institute of Nutrition and Seafood Research, PO Box 2029 Nordnes, N-5817 Bergen, Norway. pal.olsvik@nifes.noNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16107325

Citation

Olsvik, P A., et al. "MRNA Expression of Antioxidant Enzymes (SOD, CAT and GSH-Px) and Lipid Peroxidative Stress in Liver of Atlantic Salmon (Salmo Salar) Exposed to Hyperoxic Water During Smoltification." Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP, vol. 141, no. 3, 2005, pp. 314-23.
Olsvik PA, Kristensen T, Waagbø R, et al. MRNA expression of antioxidant enzymes (SOD, CAT and GSH-Px) and lipid peroxidative stress in liver of Atlantic salmon (Salmo salar) exposed to hyperoxic water during smoltification. Comp Biochem Physiol C Toxicol Pharmacol. 2005;141(3):314-23.
Olsvik, P. A., Kristensen, T., Waagbø, R., Rosseland, B. O., Tollefsen, K. E., Baeverfjord, G., & Berntssen, M. H. (2005). MRNA expression of antioxidant enzymes (SOD, CAT and GSH-Px) and lipid peroxidative stress in liver of Atlantic salmon (Salmo salar) exposed to hyperoxic water during smoltification. Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP, 141(3), 314-23.
Olsvik PA, et al. MRNA Expression of Antioxidant Enzymes (SOD, CAT and GSH-Px) and Lipid Peroxidative Stress in Liver of Atlantic Salmon (Salmo Salar) Exposed to Hyperoxic Water During Smoltification. Comp Biochem Physiol C Toxicol Pharmacol. 2005;141(3):314-23. PubMed PMID: 16107325.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - mRNA expression of antioxidant enzymes (SOD, CAT and GSH-Px) and lipid peroxidative stress in liver of Atlantic salmon (Salmo salar) exposed to hyperoxic water during smoltification. AU - Olsvik,P A, AU - Kristensen,T, AU - Waagbø,R, AU - Rosseland,B O, AU - Tollefsen,K-E, AU - Baeverfjord,G, AU - Berntssen,M H G, PY - 2005/03/14/received PY - 2005/07/18/revised PY - 2005/07/19/accepted PY - 2005/8/19/pubmed PY - 2005/10/26/medline PY - 2005/8/19/entrez SP - 314 EP - 23 JF - Comparative biochemistry and physiology. Toxicology & pharmacology : CBP JO - Comp Biochem Physiol C Toxicol Pharmacol VL - 141 IS - 3 N2 - The mRNA levels of three antioxidant genes, Cu/Zn superoxide dismutase (SOD), catalase (CAT) and phospholipid hydroperoxide glutathione peroxidase (GSH-Px), were quantified with real-time qRT-PCR in liver of Atlantic salmon Salmo salar exposed to 80% (normoxia), 105% and 130% O2 saturation for 54 days. The salmon were then translocated and exposed to 90% and 130% O2 saturation for additional 72 days during smoltification. TBARS and vitamin E levels in liver and the levels of oxidized glutathione (GSSG), total glutathione (GSH) and the resulting oxidative stress index (OSI) in blood were quantified as traditional oxidative stress markers. No significant mean normalized expression (MNE) differences of SOD, CAT or GSH-Px were found in liver after hyperoxia exposure at the two sampling times. Significantly decreased OSI was found in smolt exposed to 130% O2 saturation after 126 days (n = 18, P < 0.0001), indicating hyperoxia-induced oxidative stress. No effects were seen on growth, or on the levels of thiobarbituric reactive substances (TBARS) and vitamin E in liver after the exposure experiment. Overall, the mRNA expression of SOD, CAT and GSH-Px in liver related poorly with the hyperoxic exposure regimes, and more knowledge are needed before the expressed levels of these antioxidant genes can be applied as biomarkers of hyperoxia in Atlantic salmon. SN - 1532-0456 UR - https://www.unboundmedicine.com/medline/citation/16107325/mRNA_expression_of_antioxidant_enzymes__SOD_CAT_and_GSH_Px__and_lipid_peroxidative_stress_in_liver_of_Atlantic_salmon__Salmo_salar__exposed_to_hyperoxic_water_during_smoltification_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1532-0456(05)00171-7 DB - PRIME DP - Unbound Medicine ER -