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Crosslinked chitosan: its physical properties and the effects of matrix stiffness on chondrocyte cell morphology and proliferation.
J Biomed Mater Res A 2005; 75(3):742-53JB

Abstract

Chitosan [beta(1-4)-2 amino-2-deoxy-D-glucose], the natural polyaminosaccharide derived from N-deacetylation of chitin [beta(1-4)-2 acetamide-2-deoxy-D-glucose], has been shown to possess attractive biological and cell interactive properties. Recently chitosan and chitosan analogs have also been shown to support the growth and continued function of chondrocytes. In the present study, chitosan substrates are crosslinked with a functional diepoxide (1,4 butanediol diglycidyl ether) to alter its mechanical property, and the viability and proliferation of the canine articular chondrocytes seeded on the crosslinked surface are further assayed. Of interest is the impact of substrate stiffness on the growth and proliferation of articular canine chondrocytes. Crosslinked scaffolds were also subjected to degradation by chitosanase to examine the impact of crosslinking on enzyme-assisted degradation. The hydrophilicity and compression modulus of the crosslinked surfaces were measured via contact-angle measurements and compression tests, respectively. Scanning electron microscopy (SEM) and fluorescent staining were used to observe the proliferation and morphology of chondrocyte cells on noncrosslinked and crosslinked surfaces. The crosslinked chitosan was found to be nontoxic to chondrocytes and more hydrophilic. Its compression modulus and stiffness increased, which may improve the scaffold resistance to wear and in vivo shrinkage once implanted. The increased stiffness also seemed to serve as an additional mechanical stimulus to promote chondrocyte growth and proliferation. The cell morphology on crosslinked scaffolds seen by SEM and fluorescent stain was the typical chondrocytic rounded shape. The method proposed provides a nontoxic way to increase the mechanical strength of the chitosan scaffolds.

Authors+Show Affiliations

Department of Chemical Engineering, 207 Othmer Hall, University of Nebraska, Lincoln, NE 68588, USA. asubramanian2@unl.eduNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16110496

Citation

Subramanian, Anuradha, and Hsin-Yi Lin. "Crosslinked Chitosan: Its Physical Properties and the Effects of Matrix Stiffness On Chondrocyte Cell Morphology and Proliferation." Journal of Biomedical Materials Research. Part A, vol. 75, no. 3, 2005, pp. 742-53.
Subramanian A, Lin HY. Crosslinked chitosan: its physical properties and the effects of matrix stiffness on chondrocyte cell morphology and proliferation. J Biomed Mater Res A. 2005;75(3):742-53.
Subramanian, A., & Lin, H. Y. (2005). Crosslinked chitosan: its physical properties and the effects of matrix stiffness on chondrocyte cell morphology and proliferation. Journal of Biomedical Materials Research. Part A, 75(3), pp. 742-53.
Subramanian A, Lin HY. Crosslinked Chitosan: Its Physical Properties and the Effects of Matrix Stiffness On Chondrocyte Cell Morphology and Proliferation. J Biomed Mater Res A. 2005 Dec 1;75(3):742-53. PubMed PMID: 16110496.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Crosslinked chitosan: its physical properties and the effects of matrix stiffness on chondrocyte cell morphology and proliferation. AU - Subramanian,Anuradha, AU - Lin,Hsin-Yi, PY - 2005/8/20/pubmed PY - 2006/2/9/medline PY - 2005/8/20/entrez SP - 742 EP - 53 JF - Journal of biomedical materials research. Part A JO - J Biomed Mater Res A VL - 75 IS - 3 N2 - Chitosan [beta(1-4)-2 amino-2-deoxy-D-glucose], the natural polyaminosaccharide derived from N-deacetylation of chitin [beta(1-4)-2 acetamide-2-deoxy-D-glucose], has been shown to possess attractive biological and cell interactive properties. Recently chitosan and chitosan analogs have also been shown to support the growth and continued function of chondrocytes. In the present study, chitosan substrates are crosslinked with a functional diepoxide (1,4 butanediol diglycidyl ether) to alter its mechanical property, and the viability and proliferation of the canine articular chondrocytes seeded on the crosslinked surface are further assayed. Of interest is the impact of substrate stiffness on the growth and proliferation of articular canine chondrocytes. Crosslinked scaffolds were also subjected to degradation by chitosanase to examine the impact of crosslinking on enzyme-assisted degradation. The hydrophilicity and compression modulus of the crosslinked surfaces were measured via contact-angle measurements and compression tests, respectively. Scanning electron microscopy (SEM) and fluorescent staining were used to observe the proliferation and morphology of chondrocyte cells on noncrosslinked and crosslinked surfaces. The crosslinked chitosan was found to be nontoxic to chondrocytes and more hydrophilic. Its compression modulus and stiffness increased, which may improve the scaffold resistance to wear and in vivo shrinkage once implanted. The increased stiffness also seemed to serve as an additional mechanical stimulus to promote chondrocyte growth and proliferation. The cell morphology on crosslinked scaffolds seen by SEM and fluorescent stain was the typical chondrocytic rounded shape. The method proposed provides a nontoxic way to increase the mechanical strength of the chitosan scaffolds. SN - 1549-3296 UR - https://www.unboundmedicine.com/medline/citation/16110496/Crosslinked_chitosan:_its_physical_properties_and_the_effects_of_matrix_stiffness_on_chondrocyte_cell_morphology_and_proliferation_ L2 - https://doi.org/10.1002/jbm.a.30489 DB - PRIME DP - Unbound Medicine ER -