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Chloroquine is a potent inhibitor of SARS coronavirus infection and spread.
Virol J. 2005 Aug 22; 2:69.VJ

Abstract

BACKGROUND

Severe acute respiratory syndrome (SARS) is caused by a newly discovered coronavirus (SARS-CoV). No effective prophylactic or post-exposure therapy is currently available.

RESULTS

We report, however, that chloroquine has strong antiviral effects on SARS-CoV infection of primate cells. These inhibitory effects are observed when the cells are treated with the drug either before or after exposure to the virus, suggesting both prophylactic and therapeutic advantage. In addition to the well-known functions of chloroquine such as elevations of endosomal pH, the drug appears to interfere with terminal glycosylation of the cellular receptor, angiotensin-converting enzyme 2. This may negatively influence the virus-receptor binding and abrogate the infection, with further ramifications by the elevation of vesicular pH, resulting in the inhibition of infection and spread of SARS CoV at clinically admissible concentrations.

CONCLUSION

Chloroquine is effective in preventing the spread of SARS CoV in cell culture. Favorable inhibition of virus spread was observed when the cells were either treated with chloroquine prior to or after SARS CoV infection. In addition, the indirect immunofluorescence assay described herein represents a simple and rapid method for screening SARS-CoV antiviral compounds.

Authors+Show Affiliations

Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA. mvincent@cdc.govNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16115318

Citation

Vincent, Martin J., et al. "Chloroquine Is a Potent Inhibitor of SARS Coronavirus Infection and Spread." Virology Journal, vol. 2, 2005, p. 69.
Vincent MJ, Bergeron E, Benjannet S, et al. Chloroquine is a potent inhibitor of SARS coronavirus infection and spread. Virol J. 2005;2:69.
Vincent, M. J., Bergeron, E., Benjannet, S., Erickson, B. R., Rollin, P. E., Ksiazek, T. G., Seidah, N. G., & Nichol, S. T. (2005). Chloroquine is a potent inhibitor of SARS coronavirus infection and spread. Virology Journal, 2, 69.
Vincent MJ, et al. Chloroquine Is a Potent Inhibitor of SARS Coronavirus Infection and Spread. Virol J. 2005 Aug 22;2:69. PubMed PMID: 16115318.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chloroquine is a potent inhibitor of SARS coronavirus infection and spread. AU - Vincent,Martin J, AU - Bergeron,Eric, AU - Benjannet,Suzanne, AU - Erickson,Bobbie R, AU - Rollin,Pierre E, AU - Ksiazek,Thomas G, AU - Seidah,Nabil G, AU - Nichol,Stuart T, Y1 - 2005/08/22/ PY - 2005/07/12/received PY - 2005/08/22/accepted PY - 2005/8/24/pubmed PY - 2005/8/24/medline PY - 2005/8/24/entrez SP - 69 EP - 69 JF - Virology journal JO - Virol. J. VL - 2 N2 - BACKGROUND: Severe acute respiratory syndrome (SARS) is caused by a newly discovered coronavirus (SARS-CoV). No effective prophylactic or post-exposure therapy is currently available. RESULTS: We report, however, that chloroquine has strong antiviral effects on SARS-CoV infection of primate cells. These inhibitory effects are observed when the cells are treated with the drug either before or after exposure to the virus, suggesting both prophylactic and therapeutic advantage. In addition to the well-known functions of chloroquine such as elevations of endosomal pH, the drug appears to interfere with terminal glycosylation of the cellular receptor, angiotensin-converting enzyme 2. This may negatively influence the virus-receptor binding and abrogate the infection, with further ramifications by the elevation of vesicular pH, resulting in the inhibition of infection and spread of SARS CoV at clinically admissible concentrations. CONCLUSION: Chloroquine is effective in preventing the spread of SARS CoV in cell culture. Favorable inhibition of virus spread was observed when the cells were either treated with chloroquine prior to or after SARS CoV infection. In addition, the indirect immunofluorescence assay described herein represents a simple and rapid method for screening SARS-CoV antiviral compounds. SN - 1743-422X UR - https://www.unboundmedicine.com/medline/citation/16115318/Chloroquine_is_a_potent_inhibitor_of_SARS_coronavirus_infection_and_spread_ L2 - https://virologyj.biomedcentral.com/articles/10.1186/1743-422X-2-69 DB - PRIME DP - Unbound Medicine ER -