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[Changes of leukotriene B4 in induced sputum and plasma of patients with chronic obstructive pulmonary disease and the effects of theophylline].
Zhonghua Jie He He Hu Xi Za Zhi. 2005 Jul; 28(7):441-4.ZJ

Abstract

OBJECTIVE

To investigate the changes of leukotriene B(4) (LTB(4)) in induced sputum and plasma of patients with chronic obstructive pulmonary disease (COPD) and the effects of theophylline.

METHODS

The investigation was a prospective, randomized controlled trial. Forty stable COPD patients (group C) were randomized into a subgroup receiving oral theophylline 0.2 g twice a day for one month (group CA) and a subgroup receiving no theophylline (group CB). Fifteen age-matched healthy non-smokers (group H) were included as controls. The following measurements were performed at baseline for each group and one month later for group C: symptom and life quality scores, pulmonary function, cell counts and cell differentials in induced sputum, and concentrations of interleukin-8 (IL-8) and LTB(4) in both induced sputum and plasma by using enzyme linked immunosorbent assay (ELISA) and enzyme immunoassay (EIA).

RESULTS

Concentrations of LTB(4) in induced sputum [(794 +/- 305) pg/mg x pro] and plasma [(5,219 +/- 1,185) ng/L] in group C were significantly higher than those in group H [(347 +/- 169) pg/mg x pro, (2,283 +/- 489) ng/L, all P < 0.05]. The level of LTB(4) in induced sputum was positively correlated with the percentage of neutrophil (r = 0.453, P = 0.018) and IL-8 (r = 0.364, P = 0.047). The pre-and post-therapy concentrations of LTB(4) in induced sputum and plasma in group CA were (812 +/- 592), (657 +/- 459) pg/mg x pro and (5,422 +/- 935), (4,589 +/- 1,057) ng/L, respectively; while in group CB the concentrations were (776 +/- 227), (860 +/- 194) pg/mg x pro and (5,074 +/- 1,850), (6,063 +/- 2,450) ng/L, respectively. There were no significant changes either in the level of LTB(4) in induced sputum or in plasma in both groups (all P > 0.05).

CONCLUSIONS

The results suggest that LTB(4) is involved in airway inflammation in COPD. Theophylline is not effective in decreasing the levels of LTB(4) in both induced sputum and plasma of COPD patients.

Links

Publisher Full Text

Authors+Show Affiliations

Ding YL
Department of Respiratory Medicine, Peking University Third Hospital, Beijing 100083, China.
Yao WZ
No affiliation info available
Liu Z
No affiliation info available
Wang YZ
No affiliation info available

MeSH

AgedBronchodilator AgentsCase-Control StudiesFemaleHumansInflammationLeukotriene B4MaleMiddle AgedPlasmaProspective StudiesPulmonary Disease, Chronic ObstructiveSputumTheophylline

Pub Type(s)

English Abstract
Journal Article
Randomized Controlled Trial

Language

chi

PubMed ID

16115390

Citation

Ding, Yan-ling, et al. "[Changes of Leukotriene B4 in Induced Sputum and Plasma of Patients With Chronic Obstructive Pulmonary Disease and the Effects of Theophylline]." Zhonghua Jie He He Hu Xi Za Zhi = Zhonghua Jiehe He Huxi Zazhi = Chinese Journal of Tuberculosis and Respiratory Diseases, vol. 28, no. 7, 2005, pp. 441-4.
Ding YL, Yao WZ, Liu Z, et al. [Changes of leukotriene B4 in induced sputum and plasma of patients with chronic obstructive pulmonary disease and the effects of theophylline]. Zhonghua Jie He He Hu Xi Za Zhi. 2005;28(7):441-4.
Ding, Y. L., Yao, W. Z., Liu, Z., & Wang, Y. Z. (2005). [Changes of leukotriene B4 in induced sputum and plasma of patients with chronic obstructive pulmonary disease and the effects of theophylline]. Zhonghua Jie He He Hu Xi Za Zhi = Zhonghua Jiehe He Huxi Zazhi = Chinese Journal of Tuberculosis and Respiratory Diseases, 28(7), 441-4.
Ding YL, et al. [Changes of Leukotriene B4 in Induced Sputum and Plasma of Patients With Chronic Obstructive Pulmonary Disease and the Effects of Theophylline]. Zhonghua Jie He He Hu Xi Za Zhi. 2005;28(7):441-4. PubMed PMID: 16115390.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Changes of leukotriene B4 in induced sputum and plasma of patients with chronic obstructive pulmonary disease and the effects of theophylline]. AU - Ding,Yan-ling, AU - Yao,Wan-zhen, AU - Liu,Zheng, AU - Wang,Yu-zhu, PY - 2005/8/24/pubmed PY - 2010/9/3/medline PY - 2005/8/24/entrez SP - 441 EP - 4 JF - Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases JO - Zhonghua Jie He He Hu Xi Za Zhi VL - 28 IS - 7 N2 - OBJECTIVE: To investigate the changes of leukotriene B(4) (LTB(4)) in induced sputum and plasma of patients with chronic obstructive pulmonary disease (COPD) and the effects of theophylline. METHODS: The investigation was a prospective, randomized controlled trial. Forty stable COPD patients (group C) were randomized into a subgroup receiving oral theophylline 0.2 g twice a day for one month (group CA) and a subgroup receiving no theophylline (group CB). Fifteen age-matched healthy non-smokers (group H) were included as controls. The following measurements were performed at baseline for each group and one month later for group C: symptom and life quality scores, pulmonary function, cell counts and cell differentials in induced sputum, and concentrations of interleukin-8 (IL-8) and LTB(4) in both induced sputum and plasma by using enzyme linked immunosorbent assay (ELISA) and enzyme immunoassay (EIA). RESULTS: Concentrations of LTB(4) in induced sputum [(794 +/- 305) pg/mg x pro] and plasma [(5,219 +/- 1,185) ng/L] in group C were significantly higher than those in group H [(347 +/- 169) pg/mg x pro, (2,283 +/- 489) ng/L, all P < 0.05]. The level of LTB(4) in induced sputum was positively correlated with the percentage of neutrophil (r = 0.453, P = 0.018) and IL-8 (r = 0.364, P = 0.047). The pre-and post-therapy concentrations of LTB(4) in induced sputum and plasma in group CA were (812 +/- 592), (657 +/- 459) pg/mg x pro and (5,422 +/- 935), (4,589 +/- 1,057) ng/L, respectively; while in group CB the concentrations were (776 +/- 227), (860 +/- 194) pg/mg x pro and (5,074 +/- 1,850), (6,063 +/- 2,450) ng/L, respectively. There were no significant changes either in the level of LTB(4) in induced sputum or in plasma in both groups (all P > 0.05). CONCLUSIONS: The results suggest that LTB(4) is involved in airway inflammation in COPD. Theophylline is not effective in decreasing the levels of LTB(4) in both induced sputum and plasma of COPD patients. SN - 1001-0939 UR - https://www.unboundmedicine.com/medline/citation/16115390/[Changes_of_leukotriene_B4_in_induced_sputum_and_plasma_of_patients_with_chronic_obstructive_pulmonary_disease_and_the_effects_of_theophylline]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&amp;issn=1001-0939&amp;year=2005&amp;vol=28&amp;issue=7&amp;fpage=441 DB - PRIME DP - Unbound Medicine ER -