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Crystal structures and mutational analysis of amFP486, a cyan fluorescent protein from Anemonia majano.
Proc Natl Acad Sci U S A 2005; 102(36):12712-7PN

Abstract

Fluorescent proteins isolated from coral reef organisms can be roughly grouped into four color classes by emission, cyan, green, yellow, and red. To gain insight into the structural basis for cyan emission, the crystal structure of amFP486 (lambda(em)max = 486 nm) was determined by molecular replacement, and the model was refined at 1.65-A resolution. The electron density map reveals a chromophore formed from the tripeptide sequence -K-Y-G- that is indistinguishable from that of GFP (lambda(em)max = 509 nm). However, the chromophore environment closely parallels those of the yellow- and red-shifted homologs zFP538, DsRed, and eqFP611. Mutagenesis was performed for Glu-150, Ala-165, His-199, and Glu-217, which are immediately adjacent to the chromophore. His-199 and Ala-165 are key side chains responsible for the blue shift, presumably by localizing chromophore charge density on the phenolate moiety. Furthermore, in the H199T mutant the fluorescence quantum yield is reduced by a factor of approximately 110. The crystal structures of H199T (lambda(em)max = 515 nm) and E150Q (lambda(em)max = 506 nm) were determined. Remarkably, the H199T structure reveals that the stacking interaction of His-199 with the chromophore also controls the fluorescence efficiency, because the chromophore is statistically distributed in a 1:1 ratio between cis (fluorescent) and trans (nonfluorescent) conformations.

Authors+Show Affiliations

Department of Physics and Institute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA.No affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

16120682

Citation

Henderson, J Nathan, and S James Remington. "Crystal Structures and Mutational Analysis of amFP486, a Cyan Fluorescent Protein From Anemonia Majano." Proceedings of the National Academy of Sciences of the United States of America, vol. 102, no. 36, 2005, pp. 12712-7.
Henderson JN, Remington SJ. Crystal structures and mutational analysis of amFP486, a cyan fluorescent protein from Anemonia majano. Proc Natl Acad Sci USA. 2005;102(36):12712-7.
Henderson, J. N., & Remington, S. J. (2005). Crystal structures and mutational analysis of amFP486, a cyan fluorescent protein from Anemonia majano. Proceedings of the National Academy of Sciences of the United States of America, 102(36), pp. 12712-7.
Henderson JN, Remington SJ. Crystal Structures and Mutational Analysis of amFP486, a Cyan Fluorescent Protein From Anemonia Majano. Proc Natl Acad Sci USA. 2005 Sep 6;102(36):12712-7. PubMed PMID: 16120682.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Crystal structures and mutational analysis of amFP486, a cyan fluorescent protein from Anemonia majano. AU - Henderson,J Nathan, AU - Remington,S James, Y1 - 2005/08/24/ PY - 2005/8/27/pubmed PY - 2005/11/3/medline PY - 2005/8/27/entrez SP - 12712 EP - 7 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc. Natl. Acad. Sci. U.S.A. VL - 102 IS - 36 N2 - Fluorescent proteins isolated from coral reef organisms can be roughly grouped into four color classes by emission, cyan, green, yellow, and red. To gain insight into the structural basis for cyan emission, the crystal structure of amFP486 (lambda(em)max = 486 nm) was determined by molecular replacement, and the model was refined at 1.65-A resolution. The electron density map reveals a chromophore formed from the tripeptide sequence -K-Y-G- that is indistinguishable from that of GFP (lambda(em)max = 509 nm). However, the chromophore environment closely parallels those of the yellow- and red-shifted homologs zFP538, DsRed, and eqFP611. Mutagenesis was performed for Glu-150, Ala-165, His-199, and Glu-217, which are immediately adjacent to the chromophore. His-199 and Ala-165 are key side chains responsible for the blue shift, presumably by localizing chromophore charge density on the phenolate moiety. Furthermore, in the H199T mutant the fluorescence quantum yield is reduced by a factor of approximately 110. The crystal structures of H199T (lambda(em)max = 515 nm) and E150Q (lambda(em)max = 506 nm) were determined. Remarkably, the H199T structure reveals that the stacking interaction of His-199 with the chromophore also controls the fluorescence efficiency, because the chromophore is statistically distributed in a 1:1 ratio between cis (fluorescent) and trans (nonfluorescent) conformations. SN - 0027-8424 UR - https://www.unboundmedicine.com/medline/citation/16120682/Crystal_structures_and_mutational_analysis_of_amFP486_a_cyan_fluorescent_protein_from_Anemonia_majano_ L2 - http://www.pnas.org/cgi/pmidlookup?view=long&pmid=16120682 DB - PRIME DP - Unbound Medicine ER -