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Expression analysis and physical mapping of low-molecular-weight glutenin loci in hexaploid wheat (Triticum aestivum L.).
Genome. 2005 Jun; 48(3):401-10.G

Abstract

Gliadins and glutenins are storage proteins important in determining the bread-, noodle-, and pasta-making quality of wheat. Glutenins consist of HMW and LMW subunits. The Glu-A3, Glu-B3, and Glu-D3 loci on the short arms of chromosomes 1A, 1B, and 1D, respectively, are the major loci for LMW glutenins. To construct physical maps of the Glu-3 loci, a set of 24 high-density filters representing a 3.1x genome coverage hexaploid wheat BAC library was screened by hybridization using a probe made of 3 LMW glutenin sequences. After 2 rounds of hybridization, a subset of 536 BAC clones were selected and fingerprinted. Three developing seed cDNA libraries were also constructed. A total of 5000-6000 ESTs were generated from each library, assembled into contigs and searched by homology for LMW glutenin sequences. In total, 90 full-length LMW glutenin sequences were found to cluster into 8 distinct groups representing at least 21 different LMW glutenin subunits. A set of 24 pairs of PCR primers was designed from these groups and used as markers on the BAC clones. The combined fingerprinting and marker data were used to build the physical maps using FPC software. A total of 91 contigs comprising 254 clones were obtained and 282 clones remained singletons.

Authors+Show Affiliations

Ozdemir N
Cereal Research Centre, Agriculture and Agri-Food Canada, Winnipeg, Canada.
Cloutier S
No affiliation info available

MeSH

Chromosomes, Artificial, BacterialGene Expression ProfilingGene LibraryGenetic MarkersGlutensMolecular WeightNucleic Acid HybridizationPhysical Chromosome MappingPolyploidyTriticum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16121237

Citation

Ozdemir, Nehir, and Sylvie Cloutier. "Expression Analysis and Physical Mapping of Low-molecular-weight Glutenin Loci in Hexaploid Wheat (Triticum Aestivum L.)." Genome, vol. 48, no. 3, 2005, pp. 401-10.
Ozdemir N, Cloutier S. Expression analysis and physical mapping of low-molecular-weight glutenin loci in hexaploid wheat (Triticum aestivum L.). Genome. 2005;48(3):401-10.
Ozdemir, N., & Cloutier, S. (2005). Expression analysis and physical mapping of low-molecular-weight glutenin loci in hexaploid wheat (Triticum aestivum L.). Genome, 48(3), 401-10.
Ozdemir N, Cloutier S. Expression Analysis and Physical Mapping of Low-molecular-weight Glutenin Loci in Hexaploid Wheat (Triticum Aestivum L.). Genome. 2005;48(3):401-10. PubMed PMID: 16121237.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression analysis and physical mapping of low-molecular-weight glutenin loci in hexaploid wheat (Triticum aestivum L.). AU - Ozdemir,Nehir, AU - Cloutier,Sylvie, PY - 2005/8/27/pubmed PY - 2007/4/25/medline PY - 2005/8/27/entrez SP - 401 EP - 10 JF - Genome JO - Genome VL - 48 IS - 3 N2 - Gliadins and glutenins are storage proteins important in determining the bread-, noodle-, and pasta-making quality of wheat. Glutenins consist of HMW and LMW subunits. The Glu-A3, Glu-B3, and Glu-D3 loci on the short arms of chromosomes 1A, 1B, and 1D, respectively, are the major loci for LMW glutenins. To construct physical maps of the Glu-3 loci, a set of 24 high-density filters representing a 3.1x genome coverage hexaploid wheat BAC library was screened by hybridization using a probe made of 3 LMW glutenin sequences. After 2 rounds of hybridization, a subset of 536 BAC clones were selected and fingerprinted. Three developing seed cDNA libraries were also constructed. A total of 5000-6000 ESTs were generated from each library, assembled into contigs and searched by homology for LMW glutenin sequences. In total, 90 full-length LMW glutenin sequences were found to cluster into 8 distinct groups representing at least 21 different LMW glutenin subunits. A set of 24 pairs of PCR primers was designed from these groups and used as markers on the BAC clones. The combined fingerprinting and marker data were used to build the physical maps using FPC software. A total of 91 contigs comprising 254 clones were obtained and 282 clones remained singletons. SN - 0831-2796 UR - https://www.unboundmedicine.com/medline/citation/16121237/Expression_analysis_and_physical_mapping_of_low_molecular_weight_glutenin_loci_in_hexaploid_wheat__Triticum_aestivum_L___ DB - PRIME DP - Unbound Medicine ER -