Application of capillary zone electrophoresis with indirect UV detection to the determination of a model drug, vigabatrin, in dosage forms.
J Pharm Pharm Sci. 2005 Aug 03; 8(2):190-8.JP

Abstract

PURPOSE

Vigabatrin, an anti-epileptic drug with poor UV absorptivity, is used as a model drug to investigate parameters affecting quantitative determination of cationic drugs using capillary zone electrophoresis with indirect UV detection.

METHODS

All experiments were performed on a HP 3D CE instrument equipped with an on-column diode array UV absorbance detector. Untreated fused silica capillaries with an inner diameter of 50 microm, an outer diameter of 365 microm, and a total length of 50 cm (41.5 cm to the detector) were used. Indirect UV detection was performed at 214 nm. Operational parameters such as buffer type and concentration, its pH, probe type and concentration, and the impact of co-ions on the efficiency of separation, were studied. Sabrilex sachets and Sabril tablets were subjected to analysis in this study.

RESULTS

Optimal separation and quantification of vigabatrin was obtained using 5 mM sodium phosphate buffer containing 5 mM benzyl tri-ethyl ammonium hydroxide (BTEA) at pH 2.2, with 8-aminocaprylic acid as the internal standard. The method was linear over a range of 5-150 microg/ml (r = 0.9911) and a wider range of 100-600 microg/ml (r = 0.9937) concentration of the drug. The relative standard deviation (RSD) of migration time for 10 consecutive injections of a standard solution of vigabatrin was 0.19%. The limit of quantification (LOQ) was 5 microg/ml.

CONCLUSIONS

The method was demonstrated for quantification of vigabatrin in both tablet and sachet dosage forms and proved to be a very specific and fast (8 min) means of routine analysis of the drug in dosage forms, in assay or dissolution testing.

Authors+Show Affiliations

Shafaati A

Department of Chemistry, Gunning/Lemieux Chemistry Centre, University of Alberta, Edmonton, Alberta, Canada. ashafaati@yahoo.com

Lucy C

No affiliation info available

MeSH

Chemistry, PharmaceuticalDosage FormsElectrophoresis, CapillarySpectrophotometry, UltravioletVigabatrin

Pub Type(s)

Comparative Study

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16124930

Citation

Shafaati, Alireza, and Charles Lucy. "Application of Capillary Zone Electrophoresis With Indirect UV Detection to the Determination of a Model Drug, Vigabatrin, in Dosage Forms." Journal of Pharmacy & Pharmaceutical Sciences : a Publication of the Canadian Society for Pharmaceutical Sciences, Societe Canadienne Des Sciences Pharmaceutiques, vol. 8, no. 2, 2005, pp. 190-8.

Shafaati A, Lucy C. Application of capillary zone electrophoresis with indirect UV detection to the determination of a model drug, vigabatrin, in dosage forms. J Pharm Pharm Sci. 2005;8(2):190-8.

Shafaati, A., & Lucy, C. (2005). Application of capillary zone electrophoresis with indirect UV detection to the determination of a model drug, vigabatrin, in dosage forms. Journal of Pharmacy & Pharmaceutical Sciences : a Publication of the Canadian Society for Pharmaceutical Sciences, Societe Canadienne Des Sciences Pharmaceutiques, 8(2), 190-8.

Shafaati A, Lucy C. Application of Capillary Zone Electrophoresis With Indirect UV Detection to the Determination of a Model Drug, Vigabatrin, in Dosage Forms. J Pharm Pharm Sci. 2005 Aug 3;8(2):190-8. PubMed PMID: 16124930.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Application of capillary zone electrophoresis with indirect UV detection to the determination of a model drug, vigabatrin, in dosage forms. AU - Shafaati,Alireza, AU - Lucy,Charles, Y1 - 2005/08/03/ PY - 2005/8/30/pubmed PY - 2006/4/7/medline PY - 2005/8/30/entrez SP - 190 EP - 8 JF - Journal of pharmacy & pharmaceutical sciences : a publication of the Canadian Society for Pharmaceutical Sciences, Societe canadienne des sciences pharmaceutiques JO - J Pharm Pharm Sci VL - 8 IS - 2 N2 - PURPOSE: Vigabatrin, an anti-epileptic drug with poor UV absorptivity, is used as a model drug to investigate parameters affecting quantitative determination of cationic drugs using capillary zone electrophoresis with indirect UV detection. METHODS: All experiments were performed on a HP 3D CE instrument equipped with an on-column diode array UV absorbance detector. Untreated fused silica capillaries with an inner diameter of 50 microm, an outer diameter of 365 microm, and a total length of 50 cm (41.5 cm to the detector) were used. Indirect UV detection was performed at 214 nm. Operational parameters such as buffer type and concentration, its pH, probe type and concentration, and the impact of co-ions on the efficiency of separation, were studied. Sabrilex sachets and Sabril tablets were subjected to analysis in this study. RESULTS: Optimal separation and quantification of vigabatrin was obtained using 5 mM sodium phosphate buffer containing 5 mM benzyl tri-ethyl ammonium hydroxide (BTEA) at pH 2.2, with 8-aminocaprylic acid as the internal standard. The method was linear over a range of 5-150 microg/ml (r = 0.9911) and a wider range of 100-600 microg/ml (r = 0.9937) concentration of the drug. The relative standard deviation (RSD) of migration time for 10 consecutive injections of a standard solution of vigabatrin was 0.19%. The limit of quantification (LOQ) was 5 microg/ml. CONCLUSIONS: The method was demonstrated for quantification of vigabatrin in both tablet and sachet dosage forms and proved to be a very specific and fast (8 min) means of routine analysis of the drug in dosage forms, in assay or dissolution testing. SN - 1482-1826 UR - https://www.unboundmedicine.com/medline/citation/16124930/Application_of_capillary_zone_electrophoresis_with_indirect_UV_detection_to_the_determination_of_a_model_drug_vigabatrin_in_dosage_forms_ DB - PRIME DP - Unbound Medicine ER -

Tags

Application of capillary zone electrophoresis with indirect UV detection to the determination of a model drug, vigabatrin, in dosage forms.J Pharm Pharm Sci. 2005 Aug 03; 8(2):190-8.JP