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Analysis of cocaine and three of its metabolites in hair by gas chromatography-mass spectrometry using ion-trap detection for CI/MS/MS.
J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Nov 05; 826(1-2):17-25.JC

Abstract

A sensitive GC/CI/MS/MS method was developed for the simultaneous determination of cocaine (COC), anhydroecgonine methylester (cocaine pyrolysis product, AEME), ecgonine methylester (cocaine enzymatic hydrolysis product, EME) and cocaethylene (cocaine with ethanol trans-esterification product, COET) in human hair samples. After acid hydrolysis, hair samples were extracted with an automated solid phase extraction (SPE). The analysis of cocaine and its three metabolites was performed using an ion-trap spectrometer in positive chemical ionization with isobutane as gas reagent. The procedure was validated. Weighted linear regression was found appropriate in a concentration range of 0.10-5.00 ng/mg for AEME, 0.05-5.00 ng/mg for COC, EME and COET. The limit of detection was estimated at 0.005 ng/mg for COC and COET, at 0.025 ng/mg for EME, and at 0.050 ng/mg for AEME. Method performance was evaluated in terms of trueness and precision using quality control (QC) samples over the investigated ranges. Method selectivity and robustness were also demonstrated.

Authors+Show Affiliations

Institute of Forensic Medicine, University of Geneva, 9 Avenue de Champel, 1211 Geneva 4, Switzerland.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Validation Study

Language

eng

PubMed ID

16125477

Citation

Cognard, Emmanuelle, et al. "Analysis of Cocaine and Three of Its Metabolites in Hair By Gas Chromatography-mass Spectrometry Using Ion-trap Detection for CI/MS/MS." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 826, no. 1-2, 2005, pp. 17-25.
Cognard E, Rudaz S, Bouchonnet S, et al. Analysis of cocaine and three of its metabolites in hair by gas chromatography-mass spectrometry using ion-trap detection for CI/MS/MS. J Chromatogr B Analyt Technol Biomed Life Sci. 2005;826(1-2):17-25.
Cognard, E., Rudaz, S., Bouchonnet, S., & Staub, C. (2005). Analysis of cocaine and three of its metabolites in hair by gas chromatography-mass spectrometry using ion-trap detection for CI/MS/MS. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 826(1-2), 17-25.
Cognard E, et al. Analysis of Cocaine and Three of Its Metabolites in Hair By Gas Chromatography-mass Spectrometry Using Ion-trap Detection for CI/MS/MS. J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Nov 5;826(1-2):17-25. PubMed PMID: 16125477.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of cocaine and three of its metabolites in hair by gas chromatography-mass spectrometry using ion-trap detection for CI/MS/MS. AU - Cognard,Emmanuelle, AU - Rudaz,Serge, AU - Bouchonnet,Stéphane, AU - Staub,Christian, Y1 - 2005/08/24/ PY - 2005/03/24/received PY - 2005/07/27/revised PY - 2005/07/28/accepted PY - 2005/8/30/pubmed PY - 2005/12/20/medline PY - 2005/8/30/entrez SP - 17 EP - 25 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 826 IS - 1-2 N2 - A sensitive GC/CI/MS/MS method was developed for the simultaneous determination of cocaine (COC), anhydroecgonine methylester (cocaine pyrolysis product, AEME), ecgonine methylester (cocaine enzymatic hydrolysis product, EME) and cocaethylene (cocaine with ethanol trans-esterification product, COET) in human hair samples. After acid hydrolysis, hair samples were extracted with an automated solid phase extraction (SPE). The analysis of cocaine and its three metabolites was performed using an ion-trap spectrometer in positive chemical ionization with isobutane as gas reagent. The procedure was validated. Weighted linear regression was found appropriate in a concentration range of 0.10-5.00 ng/mg for AEME, 0.05-5.00 ng/mg for COC, EME and COET. The limit of detection was estimated at 0.005 ng/mg for COC and COET, at 0.025 ng/mg for EME, and at 0.050 ng/mg for AEME. Method performance was evaluated in terms of trueness and precision using quality control (QC) samples over the investigated ranges. Method selectivity and robustness were also demonstrated. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/16125477/Analysis_of_cocaine_and_three_of_its_metabolites_in_hair_by_gas_chromatography_mass_spectrometry_using_ion_trap_detection_for_CI/MS/MS_ DB - PRIME DP - Unbound Medicine ER -