Tags

Type your tag names separated by a space and hit enter

Protective effect of lycopene on gentamicin-induced oxidative stress and nephrotoxicity in rats.
Toxicology. 2005 Nov 15; 215(3):198-204.T

Abstract

A potential therapeutic approach to protect or reverse gentamicin-induced oxidative stress and nephrotoxicity would have more importance for clinical consequences. Therefore, the present study was designed to investigate the possible protective effects of lycopene against gentamicin-induced renal damage in rats. Male Sprague-Dawley rats were divided into four groups of six rats in each one; first group served as control. The other groups were treated intraperitoneally with gentamicin alone (100 mg kg(-1) per day) for six successive days, gentamicin for 6 days following 10 days of orally lycopene (4 mg kg(-1) per day) pre-treatment and 6-days of simultaneous lycopene and gentamicin. Biochemical and histopathological examinations were utilized for evaluation of the oxidative stress and renal nephrotoxicity. Creatinine, urea, Na(+) and K(+) levels in plasma and malondialdehyde (MDA), reduced glutathione (GSH) levels and glutathione peroxidase (GSH-Px) and catalase (CAT) activities were determined in kidney tissue. Administration of gentamicin to rats induced a marked renal failure, characterized by a significant increase in plasma creatinine and urea concentrations. The animals treated with gentamicin alone showed a significantly higher kidney MDA and lower GSH-Px and CAT activities but unaffected GSH concentrations when compared with the control group. Pre-treatment with lycopene produced amelioration in biochemical indices of nephrotoxicity in plasma. However, little changes were observed in the kidney MDA and GSH levels and GSH-Px and CAT activities when compared with the gentamicin treated group. The histological structures of the renal proximal tubules showed similar patterns. On the other hand, administration of simultaneous lycopene to rats produced amelioration in MDA and GSH levels and GSH-Px and CAT activities when compared with gentamicin group. In addition, simultaneous lycopene was found to reduce the degree of kidney tissue damage in histopathological findings. These results indicate that specially simultaneous treatment of lycopene might have produced amelioration in biochemical indices and oxidative stress parameters against gentamicin-induced nephrotoxicity, but pre-treatments with lycopene had no beneficial effects on these parameters. It was concluded that lycopene as a novel natural antioxidant might have protective effects against gentamicin-induced nephrotoxicity and oxidative stress in rats.

Authors+Show Affiliations

Firat Universitesi, Veteriner Fakültesi, Farmakoloji ve Toksikoloji Anabilim Dali, 23119 Elaziğ, Turkey. izzetkarahan@hotmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16125832

Citation

Karahan, I, et al. "Protective Effect of Lycopene On Gentamicin-induced Oxidative Stress and Nephrotoxicity in Rats." Toxicology, vol. 215, no. 3, 2005, pp. 198-204.
Karahan I, Ateşşahin A, Yilmaz S, et al. Protective effect of lycopene on gentamicin-induced oxidative stress and nephrotoxicity in rats. Toxicology. 2005;215(3):198-204.
Karahan, I., Ateşşahin, A., Yilmaz, S., Ceribaşi, A. O., & Sakin, F. (2005). Protective effect of lycopene on gentamicin-induced oxidative stress and nephrotoxicity in rats. Toxicology, 215(3), 198-204.
Karahan I, et al. Protective Effect of Lycopene On Gentamicin-induced Oxidative Stress and Nephrotoxicity in Rats. Toxicology. 2005 Nov 15;215(3):198-204. PubMed PMID: 16125832.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effect of lycopene on gentamicin-induced oxidative stress and nephrotoxicity in rats. AU - Karahan,I, AU - Ateşşahin,A, AU - Yilmaz,S, AU - Ceribaşi,A O, AU - Sakin,F, Y1 - 2005/08/26/ PY - 2005/06/03/received PY - 2005/07/08/revised PY - 2005/07/08/accepted PY - 2005/8/30/pubmed PY - 2005/12/13/medline PY - 2005/8/30/entrez SP - 198 EP - 204 JF - Toxicology JO - Toxicology VL - 215 IS - 3 N2 - A potential therapeutic approach to protect or reverse gentamicin-induced oxidative stress and nephrotoxicity would have more importance for clinical consequences. Therefore, the present study was designed to investigate the possible protective effects of lycopene against gentamicin-induced renal damage in rats. Male Sprague-Dawley rats were divided into four groups of six rats in each one; first group served as control. The other groups were treated intraperitoneally with gentamicin alone (100 mg kg(-1) per day) for six successive days, gentamicin for 6 days following 10 days of orally lycopene (4 mg kg(-1) per day) pre-treatment and 6-days of simultaneous lycopene and gentamicin. Biochemical and histopathological examinations were utilized for evaluation of the oxidative stress and renal nephrotoxicity. Creatinine, urea, Na(+) and K(+) levels in plasma and malondialdehyde (MDA), reduced glutathione (GSH) levels and glutathione peroxidase (GSH-Px) and catalase (CAT) activities were determined in kidney tissue. Administration of gentamicin to rats induced a marked renal failure, characterized by a significant increase in plasma creatinine and urea concentrations. The animals treated with gentamicin alone showed a significantly higher kidney MDA and lower GSH-Px and CAT activities but unaffected GSH concentrations when compared with the control group. Pre-treatment with lycopene produced amelioration in biochemical indices of nephrotoxicity in plasma. However, little changes were observed in the kidney MDA and GSH levels and GSH-Px and CAT activities when compared with the gentamicin treated group. The histological structures of the renal proximal tubules showed similar patterns. On the other hand, administration of simultaneous lycopene to rats produced amelioration in MDA and GSH levels and GSH-Px and CAT activities when compared with gentamicin group. In addition, simultaneous lycopene was found to reduce the degree of kidney tissue damage in histopathological findings. These results indicate that specially simultaneous treatment of lycopene might have produced amelioration in biochemical indices and oxidative stress parameters against gentamicin-induced nephrotoxicity, but pre-treatments with lycopene had no beneficial effects on these parameters. It was concluded that lycopene as a novel natural antioxidant might have protective effects against gentamicin-induced nephrotoxicity and oxidative stress in rats. SN - 0300-483X UR - https://www.unboundmedicine.com/medline/citation/16125832/Protective_effect_of_lycopene_on_gentamicin_induced_oxidative_stress_and_nephrotoxicity_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0300-483X(05)00331-8 DB - PRIME DP - Unbound Medicine ER -