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Diethylstilbestrol (DES)-induced fetal thymic atrophy in C57BL/6 mice: inhibited thymocyte differentiation and increased apoptotic cell death.
Int J Toxicol. 2005 Jul-Aug; 24(4):231-9.IJ

Abstract

Treatment of pregnant C57Bl/6 mice with 48 mu g/kg diethylstilbestrol (DES) on gestation days (GDs) 14 and 16 resulted in both decreased day 18 fetal thymic cellularity as well as alterations in thymocyte phenotype. Histopathologic examination of GD 18 fetal thymi from DES-exposed dams demonstrated a decrease in thymic size and cellularity and an increase in pyknotic nuclei, indicative of apoptosis, relative to control thymi. Thymic architecture was also altered by DES treatment with a decrease in the distinction between the cortical and medullary regions. Flow cytometric staining of day 18 thymocyte suspensions with the apoptotic marker 7-aminoactinomycin D showed a decrease in thymocyte viability after DES, and a concomitant increase of thymocytes in early apoptosis. When thymocytes were co-identified by CD4 and CD8 cell surface antigen expression, trends toward increased apoptosis were present in the CD4+CD8+ and CD4+CD8- subpopulations, and significantly increased apoptosis occurred in the CD4-CD8- and CD4-CD8+ subpopulations. These histopathologic and flow cytometric findings support enhanced apoptosis of thymocytes as a contributing factor to fetal thymic atrophy caused by DES.

Authors+Show Affiliations

Virginia Tech, College of Veterinary Medicine, Blacksburg, Virginia 24061-0442, USA. ebestema@vt.eduNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16126617

Citation

Besteman, Elizabeth G., et al. "Diethylstilbestrol (DES)-induced Fetal Thymic Atrophy in C57BL/6 Mice: Inhibited Thymocyte Differentiation and Increased Apoptotic Cell Death." International Journal of Toxicology, vol. 24, no. 4, 2005, pp. 231-9.
Besteman EG, Zimmerman KL, Holladay SD. Diethylstilbestrol (DES)-induced fetal thymic atrophy in C57BL/6 mice: inhibited thymocyte differentiation and increased apoptotic cell death. Int J Toxicol. 2005;24(4):231-9.
Besteman, E. G., Zimmerman, K. L., & Holladay, S. D. (2005). Diethylstilbestrol (DES)-induced fetal thymic atrophy in C57BL/6 mice: inhibited thymocyte differentiation and increased apoptotic cell death. International Journal of Toxicology, 24(4), 231-9.
Besteman EG, Zimmerman KL, Holladay SD. Diethylstilbestrol (DES)-induced Fetal Thymic Atrophy in C57BL/6 Mice: Inhibited Thymocyte Differentiation and Increased Apoptotic Cell Death. Int J Toxicol. 2005 Jul-Aug;24(4):231-9. PubMed PMID: 16126617.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diethylstilbestrol (DES)-induced fetal thymic atrophy in C57BL/6 mice: inhibited thymocyte differentiation and increased apoptotic cell death. AU - Besteman,Elizabeth G, AU - Zimmerman,Kurt L, AU - Holladay,Steven D, PY - 2005/8/30/pubmed PY - 2005/12/15/medline PY - 2005/8/30/entrez SP - 231 EP - 9 JF - International journal of toxicology JO - Int J Toxicol VL - 24 IS - 4 N2 - Treatment of pregnant C57Bl/6 mice with 48 mu g/kg diethylstilbestrol (DES) on gestation days (GDs) 14 and 16 resulted in both decreased day 18 fetal thymic cellularity as well as alterations in thymocyte phenotype. Histopathologic examination of GD 18 fetal thymi from DES-exposed dams demonstrated a decrease in thymic size and cellularity and an increase in pyknotic nuclei, indicative of apoptosis, relative to control thymi. Thymic architecture was also altered by DES treatment with a decrease in the distinction between the cortical and medullary regions. Flow cytometric staining of day 18 thymocyte suspensions with the apoptotic marker 7-aminoactinomycin D showed a decrease in thymocyte viability after DES, and a concomitant increase of thymocytes in early apoptosis. When thymocytes were co-identified by CD4 and CD8 cell surface antigen expression, trends toward increased apoptosis were present in the CD4+CD8+ and CD4+CD8- subpopulations, and significantly increased apoptosis occurred in the CD4-CD8- and CD4-CD8+ subpopulations. These histopathologic and flow cytometric findings support enhanced apoptosis of thymocytes as a contributing factor to fetal thymic atrophy caused by DES. SN - 1091-5818 UR - https://www.unboundmedicine.com/medline/citation/16126617/Diethylstilbestrol__DES__induced_fetal_thymic_atrophy_in_C57BL/6_mice:_inhibited_thymocyte_differentiation_and_increased_apoptotic_cell_death_ L2 - https://journals.sagepub.com/doi/10.1080/10915810591000703?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -