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The tomato Aux/IAA transcription factor IAA9 is involved in fruit development and leaf morphogenesis.
Plant Cell. 2005 Oct; 17(10):2676-92.PC

Abstract

Auxin/indole-3-acetic acid (Aux/IAA) proteins are transcriptional regulators that mediate many aspects of plant responses to auxin. While functions of most Aux/IAAs have been defined mainly by gain-of-function mutant alleles in Arabidopsis thaliana, phenotypes associated with loss-of-function mutations have been scarce and subtle. We report here that the downregulation of IAA9, a tomato (Solanum lycopersicum) gene from a distinct subfamily of Aux/IAA genes, results in a pleiotropic phenotype, consistent with its ubiquitous expression pattern. IAA9-inhibited lines have simple leaves instead of wild-type compound leaves, and fruit development is triggered before fertilization, giving rise to parthenocarpy. This indicates that IAA9 is a key mediator of leaf morphogenesis and fruit set. In addition, antisense plants displayed auxin-related growth alterations, including enhanced hypocotyl/stem elongation, increased leaf vascularization, and reduced apical dominance. Auxin dose-response assays revealed that IAA9 downregulated lines were hypersensitive to auxin, although the only early auxin-responsive gene that was found to be upregulated in the antisense lines was IAA3. The activity of the IAA3 promoter was stimulated in the IAA9 antisense genetic background, indicating that IAA9 acts in planta as a transcriptional repressor of auxin signaling. While no mutation in any member of subfamily IV has been reported to date, the phenotypes associated with the downregulation of IAA9 reveal distinct and novel roles for members of the Aux/IAA gene family.

Authors+Show Affiliations

Unité Mixte de Recherche 990, Institut National de la Recherche Agronomique/Institut National Polytechnique-Ecole Nationale Supérieure Agronomique Toulouse, 31326 Castanet-Tolosan Cedex, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16126837

Citation

Wang, Hua, et al. "The Tomato Aux/IAA Transcription Factor IAA9 Is Involved in Fruit Development and Leaf Morphogenesis." The Plant Cell, vol. 17, no. 10, 2005, pp. 2676-92.
Wang H, Jones B, Li Z, et al. The tomato Aux/IAA transcription factor IAA9 is involved in fruit development and leaf morphogenesis. Plant Cell. 2005;17(10):2676-92.
Wang, H., Jones, B., Li, Z., Frasse, P., Delalande, C., Regad, F., Chaabouni, S., Latché, A., Pech, J. C., & Bouzayen, M. (2005). The tomato Aux/IAA transcription factor IAA9 is involved in fruit development and leaf morphogenesis. The Plant Cell, 17(10), 2676-92.
Wang H, et al. The Tomato Aux/IAA Transcription Factor IAA9 Is Involved in Fruit Development and Leaf Morphogenesis. Plant Cell. 2005;17(10):2676-92. PubMed PMID: 16126837.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The tomato Aux/IAA transcription factor IAA9 is involved in fruit development and leaf morphogenesis. AU - Wang,Hua, AU - Jones,Brian, AU - Li,Zhengguo, AU - Frasse,Pierre, AU - Delalande,Corinne, AU - Regad,Farid, AU - Chaabouni,Salma, AU - Latché,Alain, AU - Pech,Jean-Claude, AU - Bouzayen,Mondher, Y1 - 2005/08/26/ PY - 2005/8/30/pubmed PY - 2006/2/24/medline PY - 2005/8/30/entrez SP - 2676 EP - 92 JF - The Plant cell JO - Plant Cell VL - 17 IS - 10 N2 - Auxin/indole-3-acetic acid (Aux/IAA) proteins are transcriptional regulators that mediate many aspects of plant responses to auxin. While functions of most Aux/IAAs have been defined mainly by gain-of-function mutant alleles in Arabidopsis thaliana, phenotypes associated with loss-of-function mutations have been scarce and subtle. We report here that the downregulation of IAA9, a tomato (Solanum lycopersicum) gene from a distinct subfamily of Aux/IAA genes, results in a pleiotropic phenotype, consistent with its ubiquitous expression pattern. IAA9-inhibited lines have simple leaves instead of wild-type compound leaves, and fruit development is triggered before fertilization, giving rise to parthenocarpy. This indicates that IAA9 is a key mediator of leaf morphogenesis and fruit set. In addition, antisense plants displayed auxin-related growth alterations, including enhanced hypocotyl/stem elongation, increased leaf vascularization, and reduced apical dominance. Auxin dose-response assays revealed that IAA9 downregulated lines were hypersensitive to auxin, although the only early auxin-responsive gene that was found to be upregulated in the antisense lines was IAA3. The activity of the IAA3 promoter was stimulated in the IAA9 antisense genetic background, indicating that IAA9 acts in planta as a transcriptional repressor of auxin signaling. While no mutation in any member of subfamily IV has been reported to date, the phenotypes associated with the downregulation of IAA9 reveal distinct and novel roles for members of the Aux/IAA gene family. SN - 1040-4651 UR - https://www.unboundmedicine.com/medline/citation/16126837/The_tomato_Aux/IAA_transcription_factor_IAA9_is_involved_in_fruit_development_and_leaf_morphogenesis_ L2 - http://www.plantcell.org/cgi/pmidlookup?view=long&pmid=16126837 DB - PRIME DP - Unbound Medicine ER -