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Significance of smooth muscle/anti-actin autoantibodies in celiac disease.
Acta Gastroenterol Latinoam 2005; 35(2):83-93AG

Abstract

BACKGROUND/AIM

Smooth muscle antibody (SMA) specific for the protein actin, a major component of the cytoskeleton of epithelial cells, is one of the most prevalent non-organ specific autoantibodies in the serum of celiac disease (CD) patients. Our aim was to explore the clinical relevance of the presence of IgA type anti-actin antibody (AAA) and SMA in a series of patients with CD.

METHODS

We evaluated frozen serum samples collected at diagnosis from 92 adult patients with CD and 52 control individuals in whom CD was excluded. Patients were re-evaluated a median time of 5 yr after treatment. IgA type AAA was detected using a modified commercial ELISA assay and IgA SMA was detected using indirect immunofluorescence on primate esophagus substrate.

RESULTS

At diagnosis, samples from CD patients had significantly higher AAA values than controls (p<0.00001). While all active CD patients had serum AAA values over the cut-off for healthy controls, we observed a very significant reduction of these antibodies after treatment (p>0.0001). AAA had a highly significant correlation with both, tissue, transglutaminase (r=0.62) and antigliadin (r=0.60, p<0.00001) antibodies as well as the severity of the intestinal injury (p<0.05). SMA was detected in sera of 35 consecutive CD patients. At diagnosis, SMA positive patients had significantly higher values of AAA (p<0.0002), increased number of autoimmune disorders (p<0.04), delayed menarche (p<0.04), lower hemoglobin levels (p<0.01), increased fecal a-I antitrypsin clearance (p<0.01) and more severe diarrhea (p<0.06). We also detected a trend to more severe complications at follow-up (p=0.059).

CONCLUSIONS

Based on our findings we suggest that the presence of increased IgA AAA serum levels is a highly sensitive marker of the disturbed architecture of intestinal epithelial cells of CD patients with a potential relevance to diagnosis and follow-up. The presence of SMA seems to define a distinct subset of CD patients with a more severe clinical outcome.

Authors+Show Affiliations

Small Bowel Section, Department of Medicine, Dr Carlos Bonorino Udaondo Gastroenterology Hospital, Buenos Aires, Argentina.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Studies
Journal Article

Language

eng

PubMed ID

16127984

Citation

Pedreira, Silvia, et al. "Significance of Smooth Muscle/anti-actin Autoantibodies in Celiac Disease." Acta Gastroenterologica Latinoamericana, vol. 35, no. 2, 2005, pp. 83-93.
Pedreira S, Sugai E, Moreno ML, et al. Significance of smooth muscle/anti-actin autoantibodies in celiac disease. Acta Gastroenterol Latinoam. 2005;35(2):83-93.
Pedreira, S., Sugai, E., Moreno, M. L., Vázquez, H., Niveloni, S., Smecuol, E., ... Bai, J. C. (2005). Significance of smooth muscle/anti-actin autoantibodies in celiac disease. Acta Gastroenterologica Latinoamericana, 35(2), pp. 83-93.
Pedreira S, et al. Significance of Smooth Muscle/anti-actin Autoantibodies in Celiac Disease. Acta Gastroenterol Latinoam. 2005;35(2):83-93. PubMed PMID: 16127984.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Significance of smooth muscle/anti-actin autoantibodies in celiac disease. AU - Pedreira,Silvia, AU - Sugai,Emilia, AU - Moreno,María Laura, AU - Vázquez,Horacio, AU - Niveloni,Sonia, AU - Smecuol,Edgardo, AU - Mazure,Roberto, AU - Kogan,Zulema, AU - Mauriño,Eduardo, AU - Bai,Julio C, PY - 2005/9/1/pubmed PY - 2005/10/27/medline PY - 2005/9/1/entrez SP - 83 EP - 93 JF - Acta gastroenterologica Latinoamericana JO - Acta Gastroenterol. Latinoam. VL - 35 IS - 2 N2 - BACKGROUND/AIM: Smooth muscle antibody (SMA) specific for the protein actin, a major component of the cytoskeleton of epithelial cells, is one of the most prevalent non-organ specific autoantibodies in the serum of celiac disease (CD) patients. Our aim was to explore the clinical relevance of the presence of IgA type anti-actin antibody (AAA) and SMA in a series of patients with CD. METHODS: We evaluated frozen serum samples collected at diagnosis from 92 adult patients with CD and 52 control individuals in whom CD was excluded. Patients were re-evaluated a median time of 5 yr after treatment. IgA type AAA was detected using a modified commercial ELISA assay and IgA SMA was detected using indirect immunofluorescence on primate esophagus substrate. RESULTS: At diagnosis, samples from CD patients had significantly higher AAA values than controls (p<0.00001). While all active CD patients had serum AAA values over the cut-off for healthy controls, we observed a very significant reduction of these antibodies after treatment (p>0.0001). AAA had a highly significant correlation with both, tissue, transglutaminase (r=0.62) and antigliadin (r=0.60, p<0.00001) antibodies as well as the severity of the intestinal injury (p<0.05). SMA was detected in sera of 35 consecutive CD patients. At diagnosis, SMA positive patients had significantly higher values of AAA (p<0.0002), increased number of autoimmune disorders (p<0.04), delayed menarche (p<0.04), lower hemoglobin levels (p<0.01), increased fecal a-I antitrypsin clearance (p<0.01) and more severe diarrhea (p<0.06). We also detected a trend to more severe complications at follow-up (p=0.059). CONCLUSIONS: Based on our findings we suggest that the presence of increased IgA AAA serum levels is a highly sensitive marker of the disturbed architecture of intestinal epithelial cells of CD patients with a potential relevance to diagnosis and follow-up. The presence of SMA seems to define a distinct subset of CD patients with a more severe clinical outcome. SN - 0300-9033 UR - https://www.unboundmedicine.com/medline/citation/16127984/Significance_of_smooth_muscle/anti_actin_autoantibodies_in_celiac_disease_ L2 - http://www.diseaseinfosearch.org/result/1186 DB - PRIME DP - Unbound Medicine ER -