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Effects of 84-days of bedrest and resistance training on single muscle fibre myosin heavy chain distribution in human vastus lateralis and soleus muscles.
Acta Physiol Scand. 2005 Sep; 185(1):61-9.AP

Abstract

AIM

This investigation determined the effects of 84 days of bedrest on the composition of myosin heavy chain (MHC) in single skeletal muscle fibres with and without a resistance-training countermeasure programme.

METHODS

Muscle biopsies were obtained from the m. vastus lateralis (VL) and m. soleus (SOL) before and after 84 days of bedrest. While control (BR) subjects (VL n = 9; SOL n = 3) refrained from exercise, BRE subjects (VL n = 8; SOL n = 3) performed knee extensor and plantar flexor resistance exercise every third day. Approximately 110 fibres per sample were analysed for MHC composition using SDS-PAGE.

RESULTS

BR-VL had 16 and 14% decreases (P < 0.05) in MHC I and IIa fibres, respectively. There were 10% increases (P < 0.05) in MHC I/IIa, IIa/IIx, I/IIa/IIx, and a approximately 30% increase (P < 0.05) in total hybrid fibres. BRE-VL showed a 15% reduction (P < 0.05) in MHC I fibres, no change in MHC IIa fibres, and a 13% increase (P < 0.05) in total hybrids. BR-SOL had a 19% decrease (P < 0.05) in MHC I fibres with a 22% increase in total hybrids. BRE-SOL showed no change in MHC composition across all fibre types.

CONCLUSION

These data suggest that the exercise countermeasures programme prevented MHC shifts in the SOL and mitigated MHC shifts in the VL. Furthermore, in the VL it appears that the resistance training programme employed in this investigation during bedrest, emphasized the use of MHC IIa phenotype muscle fibres.

Authors+Show Affiliations

Human Performance Laboratory, Ball State University, Muncie, IN 47306, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

16128698

Citation

Gallagher, P, et al. "Effects of 84-days of Bedrest and Resistance Training On Single Muscle Fibre Myosin Heavy Chain Distribution in Human Vastus Lateralis and Soleus Muscles." Acta Physiologica Scandinavica, vol. 185, no. 1, 2005, pp. 61-9.
Gallagher P, Trappe S, Harber M, et al. Effects of 84-days of bedrest and resistance training on single muscle fibre myosin heavy chain distribution in human vastus lateralis and soleus muscles. Acta Physiol Scand. 2005;185(1):61-9.
Gallagher, P., Trappe, S., Harber, M., Creer, A., Mazzetti, S., Trappe, T., Alkner, B., & Tesch, P. (2005). Effects of 84-days of bedrest and resistance training on single muscle fibre myosin heavy chain distribution in human vastus lateralis and soleus muscles. Acta Physiologica Scandinavica, 185(1), 61-9.
Gallagher P, et al. Effects of 84-days of Bedrest and Resistance Training On Single Muscle Fibre Myosin Heavy Chain Distribution in Human Vastus Lateralis and Soleus Muscles. Acta Physiol Scand. 2005;185(1):61-9. PubMed PMID: 16128698.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of 84-days of bedrest and resistance training on single muscle fibre myosin heavy chain distribution in human vastus lateralis and soleus muscles. AU - Gallagher,P, AU - Trappe,S, AU - Harber,M, AU - Creer,A, AU - Mazzetti,S, AU - Trappe,T, AU - Alkner,B, AU - Tesch,P, PY - 2005/9/1/pubmed PY - 2006/2/10/medline PY - 2005/9/1/entrez SP - 61 EP - 9 JF - Acta physiologica Scandinavica JO - Acta Physiol. Scand. VL - 185 IS - 1 N2 - AIM: This investigation determined the effects of 84 days of bedrest on the composition of myosin heavy chain (MHC) in single skeletal muscle fibres with and without a resistance-training countermeasure programme. METHODS: Muscle biopsies were obtained from the m. vastus lateralis (VL) and m. soleus (SOL) before and after 84 days of bedrest. While control (BR) subjects (VL n = 9; SOL n = 3) refrained from exercise, BRE subjects (VL n = 8; SOL n = 3) performed knee extensor and plantar flexor resistance exercise every third day. Approximately 110 fibres per sample were analysed for MHC composition using SDS-PAGE. RESULTS: BR-VL had 16 and 14% decreases (P < 0.05) in MHC I and IIa fibres, respectively. There were 10% increases (P < 0.05) in MHC I/IIa, IIa/IIx, I/IIa/IIx, and a approximately 30% increase (P < 0.05) in total hybrid fibres. BRE-VL showed a 15% reduction (P < 0.05) in MHC I fibres, no change in MHC IIa fibres, and a 13% increase (P < 0.05) in total hybrids. BR-SOL had a 19% decrease (P < 0.05) in MHC I fibres with a 22% increase in total hybrids. BRE-SOL showed no change in MHC composition across all fibre types. CONCLUSION: These data suggest that the exercise countermeasures programme prevented MHC shifts in the SOL and mitigated MHC shifts in the VL. Furthermore, in the VL it appears that the resistance training programme employed in this investigation during bedrest, emphasized the use of MHC IIa phenotype muscle fibres. SN - 0001-6772 UR - https://www.unboundmedicine.com/medline/citation/16128698/Effects_of_84_days_of_bedrest_and_resistance_training_on_single_muscle_fibre_myosin_heavy_chain_distribution_in_human_vastus_lateralis_and_soleus_muscles_ L2 - https://doi.org/10.1111/j.1365-201X.2005.01457.x DB - PRIME DP - Unbound Medicine ER -