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Protection of oxidative preconditioning against apoptosis induced by H2O2 in PC12 cells: mechanisms via MMP, ROS, and Bcl-2.
Brain Res 2005; 1057(1-2):57-64BR

Abstract

The present study is designed to investigate the effects of preconditioning with different doses of hydrogen peroxide (H2O2) on oxidative stress-induced apoptosis and the changes in mitochondrial membrane potential (MMP), intracellular reactive oxygen species (ROS) level, and expression of Bcl-2 during H2O2 preconditioning in rat pheochromocytoma (PC12) cells. It was shown that (1) H2O2 induced apoptosis in PC12 cells in a dose-dependent manner; (2) the preconditioning with 10 micromol L(-1) or 20 micromol L(-1) H2O2 can significantly protect PC12 cells against apoptosis induced by 50 or 100 micromol L(-1) H2O2, low (5 micromol L(-1)) and higher (30 micromol L(-1)) concentrations of H2O2 had no cytoprotections; (3) high concentration (100 micromol L(-1)) of H2O2 reduced MMP and expression of Bcl-2, and increased ROS level, but these effects were blocked by preconditioning with 10 micromol L(-1) H2O2; (4) the preconditioning with 10 micromol L(-1) H2O2 induced overexpression of Bcl-2. These results suggested that the preconditioning with low dose of H2O2 could protect the oxidative stress-induced PC12 cells apoptosis not only by preventing the reduction of MMP and expression of Bcl-2 as well as increase in ROS level, but also through overexpression of Bcl-2. It was indicated that overexpression of Bcl-2 may play a key role in the cytoprotection induced by preconditioning with low dose of H2O2 in PC12 cells.

Authors+Show Affiliations

Department of Physiology, Zhongshan Medical College, Sun Yat-sen University, Guangzhou 510080, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

16129420

Citation

Tang, Xiao-Qing, et al. "Protection of Oxidative Preconditioning Against Apoptosis Induced By H2O2 in PC12 Cells: Mechanisms Via MMP, ROS, and Bcl-2." Brain Research, vol. 1057, no. 1-2, 2005, pp. 57-64.
Tang XQ, Feng JQ, Chen J, et al. Protection of oxidative preconditioning against apoptosis induced by H2O2 in PC12 cells: mechanisms via MMP, ROS, and Bcl-2. Brain Res. 2005;1057(1-2):57-64.
Tang, X. Q., Feng, J. Q., Chen, J., Chen, P. X., Zhi, J. L., Cui, Y., ... Yu, H. M. (2005). Protection of oxidative preconditioning against apoptosis induced by H2O2 in PC12 cells: mechanisms via MMP, ROS, and Bcl-2. Brain Research, 1057(1-2), pp. 57-64.
Tang XQ, et al. Protection of Oxidative Preconditioning Against Apoptosis Induced By H2O2 in PC12 Cells: Mechanisms Via MMP, ROS, and Bcl-2. Brain Res. 2005 Sep 28;1057(1-2):57-64. PubMed PMID: 16129420.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protection of oxidative preconditioning against apoptosis induced by H2O2 in PC12 cells: mechanisms via MMP, ROS, and Bcl-2. AU - Tang,Xiao-Qing, AU - Feng,Jian-Qiang, AU - Chen,Jin, AU - Chen,Pei-Xi, AU - Zhi,Jun-Li, AU - Cui,Yu, AU - Guo,Rui-Xian, AU - Yu,Hui-Min, PY - 2005/01/05/received PY - 2005/07/16/revised PY - 2005/07/19/accepted PY - 2005/9/1/pubmed PY - 2006/1/5/medline PY - 2005/9/1/entrez SP - 57 EP - 64 JF - Brain research JO - Brain Res. VL - 1057 IS - 1-2 N2 - The present study is designed to investigate the effects of preconditioning with different doses of hydrogen peroxide (H2O2) on oxidative stress-induced apoptosis and the changes in mitochondrial membrane potential (MMP), intracellular reactive oxygen species (ROS) level, and expression of Bcl-2 during H2O2 preconditioning in rat pheochromocytoma (PC12) cells. It was shown that (1) H2O2 induced apoptosis in PC12 cells in a dose-dependent manner; (2) the preconditioning with 10 micromol L(-1) or 20 micromol L(-1) H2O2 can significantly protect PC12 cells against apoptosis induced by 50 or 100 micromol L(-1) H2O2, low (5 micromol L(-1)) and higher (30 micromol L(-1)) concentrations of H2O2 had no cytoprotections; (3) high concentration (100 micromol L(-1)) of H2O2 reduced MMP and expression of Bcl-2, and increased ROS level, but these effects were blocked by preconditioning with 10 micromol L(-1) H2O2; (4) the preconditioning with 10 micromol L(-1) H2O2 induced overexpression of Bcl-2. These results suggested that the preconditioning with low dose of H2O2 could protect the oxidative stress-induced PC12 cells apoptosis not only by preventing the reduction of MMP and expression of Bcl-2 as well as increase in ROS level, but also through overexpression of Bcl-2. It was indicated that overexpression of Bcl-2 may play a key role in the cytoprotection induced by preconditioning with low dose of H2O2 in PC12 cells. SN - 0006-8993 UR - https://www.unboundmedicine.com/medline/citation/16129420/Protection_of_oxidative_preconditioning_against_apoptosis_induced_by_H2O2_in_PC12_cells:_mechanisms_via_MMP_ROS_and_Bcl_2_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-8993(05)01092-9 DB - PRIME DP - Unbound Medicine ER -