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Simultaneous determination of bupivacaine, mepivacain, prilocaine and ropivacain in human serum by liquid chromatography-tandem mass spectrometry.
J Chromatogr A. 2005 Sep 23; 1088(1-2):126-30.JC

Abstract

A liquid chromatography-tandem mass spectrometric (LC-MS-MS) method with a rapid and simple sample preparation was developed and validated for the simultaneous determination of the local anesthetics bupivacaine, mepivacaine, prilocaine and ropivacaine in human serum. An external calibration was used. The mass spectrometer was operated in the multiple reaction monitoring mode. A good quadratic response over the range of 1.0-200.0 ng/ml was demonstrated. The accuracy for bupivacaine ranged from 93.2 to 105.7%, for mepivacaine from 96.2 to 104.3%, for prilocaine from 94.6 to 105.7% and for ropivacaine from 94.3 to 104.0%, respectively. The limit of quantification was 1.0 ng/ml for all substances. This method is suitable for pharmacokinetic studies.

Authors+Show Affiliations

Institute of Clinical Pharmacology, Medical Faculty Carl Gustav Carus, Technical University Dresden, Dresden, Germany. anna.koehler@gmx.deNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16130740

Citation

Koehler, A, et al. "Simultaneous Determination of Bupivacaine, Mepivacain, Prilocaine and Ropivacain in Human Serum By Liquid Chromatography-tandem Mass Spectrometry." Journal of Chromatography. A, vol. 1088, no. 1-2, 2005, pp. 126-30.
Koehler A, Oertel R, Kirch W. Simultaneous determination of bupivacaine, mepivacain, prilocaine and ropivacain in human serum by liquid chromatography-tandem mass spectrometry. J Chromatogr A. 2005;1088(1-2):126-30.
Koehler, A., Oertel, R., & Kirch, W. (2005). Simultaneous determination of bupivacaine, mepivacain, prilocaine and ropivacain in human serum by liquid chromatography-tandem mass spectrometry. Journal of Chromatography. A, 1088(1-2), 126-30.
Koehler A, Oertel R, Kirch W. Simultaneous Determination of Bupivacaine, Mepivacain, Prilocaine and Ropivacain in Human Serum By Liquid Chromatography-tandem Mass Spectrometry. J Chromatogr A. 2005 Sep 23;1088(1-2):126-30. PubMed PMID: 16130740.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous determination of bupivacaine, mepivacain, prilocaine and ropivacain in human serum by liquid chromatography-tandem mass spectrometry. AU - Koehler,A, AU - Oertel,R, AU - Kirch,W, PY - 2005/9/1/pubmed PY - 2005/10/29/medline PY - 2005/9/1/entrez SP - 126 EP - 30 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1088 IS - 1-2 N2 - A liquid chromatography-tandem mass spectrometric (LC-MS-MS) method with a rapid and simple sample preparation was developed and validated for the simultaneous determination of the local anesthetics bupivacaine, mepivacaine, prilocaine and ropivacaine in human serum. An external calibration was used. The mass spectrometer was operated in the multiple reaction monitoring mode. A good quadratic response over the range of 1.0-200.0 ng/ml was demonstrated. The accuracy for bupivacaine ranged from 93.2 to 105.7%, for mepivacaine from 96.2 to 104.3%, for prilocaine from 94.6 to 105.7% and for ropivacaine from 94.3 to 104.0%, respectively. The limit of quantification was 1.0 ng/ml for all substances. This method is suitable for pharmacokinetic studies. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/16130740/Simultaneous_determination_of_bupivacaine_mepivacain_prilocaine_and_ropivacain_in_human_serum_by_liquid_chromatography_tandem_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(05)00649-7 DB - PRIME DP - Unbound Medicine ER -