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Introgression of wheat DNA markers from A, B and D genomes in early generation progeny of Aegilops cylindrica Host x Triticum aestivum L. hybrids.
Theor Appl Genet. 2005 Nov; 111(7):1338-46.TA

Abstract

Introgression from allohexaploid wheat (Triticum aestivum L., AABBDD) to allotetraploid jointed goatgrass (Aegilops cylindrica Host, CCDD) can take place in areas where the two species grow in sympatry and hybridize. Wheat and Ae. cylindrica share the D genome, issued from the common diploid ancestor Aegilops tauschii Coss. It has been proposed that the A and B genome of bread wheat are secure places to insert transgenes to avoid their introgression into Ae. cylindrica because during meiosis in pentaploid hybrids, A and B genome chromosomes form univalents and tend to be eliminated whereas recombination takes place only in D genome chromosomes. Wheat random amplified polymorphic DNA (RAPD) fragments, detected in intergeneric hybrids and introgressed to the first backcross generation with Ae. cylindrica as the recurrent parent and having a euploid Ae. cylindrica chromosome number or one supernumerary chromosome, were assigned to wheat chromosomes using Chinese Spring nulli-tetrasomic wheat lines. Introgressed fragments were not limited to the D genome of wheat, but specific fragments of A and B genomes were also present in the BC1. Their presence indicates that DNA from any of the wheat genomes can introgress into Ae. cylindrica. Successfully located RAPD fragments were then converted into highly specific and easy-to-use sequence characterised amplified regions (SCARs) through sequencing and primer design. Subsequently these markers were used to characterise introgression of wheat DNA into a BC1S1 family. Implications for risk assessment of genetically modified wheat are discussed.

Authors+Show Affiliations

Laboratoire de Botanique Evolutive, Institut de Botanique, Université de Neuchâtel, Neuchâtel, Switzerland. nicola.schoenenberger@unine.chNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16133306

Citation

Schoenenberger, N, et al. "Introgression of Wheat DNA Markers From A, B and D Genomes in Early Generation Progeny of Aegilops Cylindrica Host X Triticum Aestivum L. Hybrids." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 111, no. 7, 2005, pp. 1338-46.
Schoenenberger N, Felber F, Savova-Bianchi D, et al. Introgression of wheat DNA markers from A, B and D genomes in early generation progeny of Aegilops cylindrica Host x Triticum aestivum L. hybrids. Theor Appl Genet. 2005;111(7):1338-46.
Schoenenberger, N., Felber, F., Savova-Bianchi, D., & Guadagnuolo, R. (2005). Introgression of wheat DNA markers from A, B and D genomes in early generation progeny of Aegilops cylindrica Host x Triticum aestivum L. hybrids. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 111(7), 1338-46.
Schoenenberger N, et al. Introgression of Wheat DNA Markers From A, B and D Genomes in Early Generation Progeny of Aegilops Cylindrica Host X Triticum Aestivum L. Hybrids. Theor Appl Genet. 2005;111(7):1338-46. PubMed PMID: 16133306.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Introgression of wheat DNA markers from A, B and D genomes in early generation progeny of Aegilops cylindrica Host x Triticum aestivum L. hybrids. AU - Schoenenberger,N, AU - Felber,F, AU - Savova-Bianchi,D, AU - Guadagnuolo,R, Y1 - 2005/11/15/ PY - 2005/04/26/received PY - 2005/07/25/accepted PY - 2005/9/1/pubmed PY - 2006/6/13/medline PY - 2005/9/1/entrez SP - 1338 EP - 46 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 111 IS - 7 N2 - Introgression from allohexaploid wheat (Triticum aestivum L., AABBDD) to allotetraploid jointed goatgrass (Aegilops cylindrica Host, CCDD) can take place in areas where the two species grow in sympatry and hybridize. Wheat and Ae. cylindrica share the D genome, issued from the common diploid ancestor Aegilops tauschii Coss. It has been proposed that the A and B genome of bread wheat are secure places to insert transgenes to avoid their introgression into Ae. cylindrica because during meiosis in pentaploid hybrids, A and B genome chromosomes form univalents and tend to be eliminated whereas recombination takes place only in D genome chromosomes. Wheat random amplified polymorphic DNA (RAPD) fragments, detected in intergeneric hybrids and introgressed to the first backcross generation with Ae. cylindrica as the recurrent parent and having a euploid Ae. cylindrica chromosome number or one supernumerary chromosome, were assigned to wheat chromosomes using Chinese Spring nulli-tetrasomic wheat lines. Introgressed fragments were not limited to the D genome of wheat, but specific fragments of A and B genomes were also present in the BC1. Their presence indicates that DNA from any of the wheat genomes can introgress into Ae. cylindrica. Successfully located RAPD fragments were then converted into highly specific and easy-to-use sequence characterised amplified regions (SCARs) through sequencing and primer design. Subsequently these markers were used to characterise introgression of wheat DNA into a BC1S1 family. Implications for risk assessment of genetically modified wheat are discussed. SN - 0040-5752 UR - https://www.unboundmedicine.com/medline/citation/16133306/Introgression_of_wheat_DNA_markers_from_A_B_and_D_genomes_in_early_generation_progeny_of_Aegilops_cylindrica_Host_x_Triticum_aestivum_L__hybrids_ L2 - https://dx.doi.org/10.1007/s00122-005-0063-7 DB - PRIME DP - Unbound Medicine ER -