TY - JOUR T1 - Double-antigen sandwich ELISA for detection of antibodies to SARS-associated coronavirus in human serum. AU - Chen,S, AU - Lu,D, AU - Zhang,M, AU - Che,J, AU - Yin,Z, AU - Zhang,S, AU - Zhang,W, AU - Bo,X, AU - Ding,Y, AU - Wang,S, PY - 2005/9/1/pubmed PY - 2005/12/16/medline PY - 2005/9/1/entrez SP - 549 EP - 53 JF - European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology JO - Eur J Clin Microbiol Infect Dis VL - 24 IS - 8 N2 - The study presented here was conducted to evaluate the performance of a double-antigen sandwich ELISA to detect antibodies in human serum against the coronavirus associated with severe acute respiratory syndrome (SARS). A recombinant partial nucleocapsid protein of SARS-associated coronavirus was used as a serodiagnostic antigen in the ELISA. A total of 2892 clinical serum samples were tested with the ELISA kit, which positively identified 25 of 35 (71.4%) samples of patients with confirmed SARS infection, 286 of 407 (70%) samples of patients suspected of having SARS, 229 of 302 (75.8%) samples of convalescent SARS patients, and 0 of 544 samples obtained from healthcare workers; only 1 of 1604 clinical samples obtained from patients with other diseases demonstrated a weakly positive result. These results indicate that the double-antigen sandwich ELISA is an effective screening method for the serodiagnosis of SARS-associated coronavirus. SN - 0934-9723 UR - https://www.unboundmedicine.com/medline/citation/16133409/Double_antigen_sandwich_ELISA_for_detection_of_antibodies_to_SARS_associated_coronavirus_in_human_serum_ DB - PRIME DP - Unbound Medicine ER -