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Capillary blood beta-hydroxybutyrate measurement by reagent strip in diagnosing diabetic ketoacidosis.
Clin Lab Sci. 2005 Summer; 18(3):139-44.CL

Abstract

OBJECTIVE

To compare blood beta-hydroxybutyrate (beta-OHB) levels measured by reagent strip with serum ketone levels assessed by the nitroprusside reaction in diagnosing diabetic ketoacidosis (DKA).

DESIGN

Prospective study of DKA in 19 Thai diabetic patients from September 2001 until August 2002.

SETTING

A university hospital.

PATIENTS

Nineteen patients with DKA and ten patients with metabolic acidosis from other causes.

INTERVENTIONS

Capillary blood alpha-OHB was measured by a blood ketone meter (MediSense Optium). Concurrently, serum ketone was measured semiquantitatively by nitroprusside reaction (Ketostix, N-multistix, and Labstix).

MAIN OUTCOME MEASURE

Sensitivity, specificity, and ROC curve of both methods in diagnosing DKA.

RESULT

Mean age +/- SD of DKA patients was 45.6 +/- 16.95 years. Plasma glucose was 675.25 +/- 188.15 mg/dL, arterial blood pH 7.19 +/- 0.12, anion gap and serum bicarbonate 29.93 +/- 4.90 and 8 +/- 3.35 mmol/L. Serum ketone was moderately to markedly positive in most cases. Capillary beta-OHB ranged from 2.4 to >6 mmol/L. The sensitivity and specificity of serum ketone by nitroprusside reaction in diagnosing DKA were 95% and 100%. The sensitivity and specificity of capillary beta-OHB was 90% and 100% respectively. The areas under ROC curves of serum ketone and capillary beta-OHB were 0.975 and 0.950 (NS) respectively.

CONCLUSION

Serum ketone and blood beta-OHB measurement are equally effective in diagnosing DKA among uncomplicated cases.

Authors+Show Affiliations

Endocrinology and Metabolism, Mahidol University, Bangkok, Thailand. tpuntip@hotmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

16134474

Citation

Tantiwong, Puntip, et al. "Capillary Blood Beta-hydroxybutyrate Measurement By Reagent Strip in Diagnosing Diabetic Ketoacidosis." Clinical Laboratory Science : Journal of the American Society for Medical Technology, vol. 18, no. 3, 2005, pp. 139-44.
Tantiwong P, Puavilai G, Ongphiphadhanakul B, et al. Capillary blood beta-hydroxybutyrate measurement by reagent strip in diagnosing diabetic ketoacidosis. Clin Lab Sci. 2005;18(3):139-44.
Tantiwong, P., Puavilai, G., Ongphiphadhanakul, B., Bunnag, P., & Ngarmukos, C. (2005). Capillary blood beta-hydroxybutyrate measurement by reagent strip in diagnosing diabetic ketoacidosis. Clinical Laboratory Science : Journal of the American Society for Medical Technology, 18(3), 139-44.
Tantiwong P, et al. Capillary Blood Beta-hydroxybutyrate Measurement By Reagent Strip in Diagnosing Diabetic Ketoacidosis. Clin Lab Sci. 2005;18(3):139-44. PubMed PMID: 16134474.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Capillary blood beta-hydroxybutyrate measurement by reagent strip in diagnosing diabetic ketoacidosis. AU - Tantiwong,Puntip, AU - Puavilai,Gobchai, AU - Ongphiphadhanakul,Boonsong, AU - Bunnag,Pongamorn, AU - Ngarmukos,Chardpraorn, PY - 2005/9/2/pubmed PY - 2005/9/21/medline PY - 2005/9/2/entrez SP - 139 EP - 44 JF - Clinical laboratory science : journal of the American Society for Medical Technology JO - Clin Lab Sci VL - 18 IS - 3 N2 - OBJECTIVE: To compare blood beta-hydroxybutyrate (beta-OHB) levels measured by reagent strip with serum ketone levels assessed by the nitroprusside reaction in diagnosing diabetic ketoacidosis (DKA). DESIGN: Prospective study of DKA in 19 Thai diabetic patients from September 2001 until August 2002. SETTING: A university hospital. PATIENTS: Nineteen patients with DKA and ten patients with metabolic acidosis from other causes. INTERVENTIONS: Capillary blood alpha-OHB was measured by a blood ketone meter (MediSense Optium). Concurrently, serum ketone was measured semiquantitatively by nitroprusside reaction (Ketostix, N-multistix, and Labstix). MAIN OUTCOME MEASURE: Sensitivity, specificity, and ROC curve of both methods in diagnosing DKA. RESULT: Mean age +/- SD of DKA patients was 45.6 +/- 16.95 years. Plasma glucose was 675.25 +/- 188.15 mg/dL, arterial blood pH 7.19 +/- 0.12, anion gap and serum bicarbonate 29.93 +/- 4.90 and 8 +/- 3.35 mmol/L. Serum ketone was moderately to markedly positive in most cases. Capillary beta-OHB ranged from 2.4 to >6 mmol/L. The sensitivity and specificity of serum ketone by nitroprusside reaction in diagnosing DKA were 95% and 100%. The sensitivity and specificity of capillary beta-OHB was 90% and 100% respectively. The areas under ROC curves of serum ketone and capillary beta-OHB were 0.975 and 0.950 (NS) respectively. CONCLUSION: Serum ketone and blood beta-OHB measurement are equally effective in diagnosing DKA among uncomplicated cases. SN - 0894-959X UR - https://www.unboundmedicine.com/medline/citation/16134474/Capillary_blood_beta_hydroxybutyrate_measurement_by_reagent_strip_in_diagnosing_diabetic_ketoacidosis_ DB - PRIME DP - Unbound Medicine ER -