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Inhibition of apoptosis-inducing factor translocation is involved in protective effects of hepatocyte growth factor against excitotoxic cell death in cultured hippocampal neurons.
J Neurochem. 2005 Dec; 95(5):1277-86.JN

Abstract

Although hepatocyte growth factor (HGF) and its receptor are expressed in various regions of the brain, their effects and mechanism of action under pathological conditions remain to be determined. Over-activation of the N-methyl-d-aspartate (NMDA) receptor, an ionotropic glutamate receptor, has been implicated in a variety of neurological and neurodegenerative disorders. We investigated the effects of HGF on the NMDA-induced cell death in cultured hippocampal neurons and sought to explore their mechanisms. NMDA-induced cell death and increase in the number of terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL)-positive cells were prevented by HGF treatment. Although neither the total amounts nor the mitochondrial localization of Bax, Bcl-2 and Bcl-xL were affected, caspase 3 activity was increased after NMDA exposure. Treatment with HGF partially prevented this NMDA-induced activation of caspase 3. Although the amount of apoptosis-inducing factor (AIF) was not altered, translocation of AIF into the nucleus was detected after NMDA exposure. This NMDA-induced AIF translocation was reduced by treatment with HGF. In addition, increased poly(ADP-ribose) polymer formation after NMDA exposure was attenuated by treatment with HGF. These results suggest that the protective effects of HGF against NMDA-induced neurotoxicity are mediated via the partial prevention of caspase 3 activity and the inhibition of AIF translocation to the nucleus.

Authors+Show Affiliations

Department of Molecular and Cellular Pharmacology, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16135073

Citation

Ishihara, Naoko, et al. "Inhibition of Apoptosis-inducing Factor Translocation Is Involved in Protective Effects of Hepatocyte Growth Factor Against Excitotoxic Cell Death in Cultured Hippocampal Neurons." Journal of Neurochemistry, vol. 95, no. 5, 2005, pp. 1277-86.
Ishihara N, Takagi N, Niimura M, et al. Inhibition of apoptosis-inducing factor translocation is involved in protective effects of hepatocyte growth factor against excitotoxic cell death in cultured hippocampal neurons. J Neurochem. 2005;95(5):1277-86.
Ishihara, N., Takagi, N., Niimura, M., Takagi, K., Nakano, M., Tanonaka, K., Funakoshi, H., Matsumoto, K., Nakamura, T., & Takeo, S. (2005). Inhibition of apoptosis-inducing factor translocation is involved in protective effects of hepatocyte growth factor against excitotoxic cell death in cultured hippocampal neurons. Journal of Neurochemistry, 95(5), 1277-86.
Ishihara N, et al. Inhibition of Apoptosis-inducing Factor Translocation Is Involved in Protective Effects of Hepatocyte Growth Factor Against Excitotoxic Cell Death in Cultured Hippocampal Neurons. J Neurochem. 2005;95(5):1277-86. PubMed PMID: 16135073.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibition of apoptosis-inducing factor translocation is involved in protective effects of hepatocyte growth factor against excitotoxic cell death in cultured hippocampal neurons. AU - Ishihara,Naoko, AU - Takagi,Norio, AU - Niimura,Makiko, AU - Takagi,Keiko, AU - Nakano,Midori, AU - Tanonaka,Kouichi, AU - Funakoshi,Hiroshi, AU - Matsumoto,Kunio, AU - Nakamura,Toshikazu, AU - Takeo,Satoshi, Y1 - 2005/08/31/ PY - 2005/9/2/pubmed PY - 2006/2/8/medline PY - 2005/9/2/entrez SP - 1277 EP - 86 JF - Journal of neurochemistry JO - J Neurochem VL - 95 IS - 5 N2 - Although hepatocyte growth factor (HGF) and its receptor are expressed in various regions of the brain, their effects and mechanism of action under pathological conditions remain to be determined. Over-activation of the N-methyl-d-aspartate (NMDA) receptor, an ionotropic glutamate receptor, has been implicated in a variety of neurological and neurodegenerative disorders. We investigated the effects of HGF on the NMDA-induced cell death in cultured hippocampal neurons and sought to explore their mechanisms. NMDA-induced cell death and increase in the number of terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL)-positive cells were prevented by HGF treatment. Although neither the total amounts nor the mitochondrial localization of Bax, Bcl-2 and Bcl-xL were affected, caspase 3 activity was increased after NMDA exposure. Treatment with HGF partially prevented this NMDA-induced activation of caspase 3. Although the amount of apoptosis-inducing factor (AIF) was not altered, translocation of AIF into the nucleus was detected after NMDA exposure. This NMDA-induced AIF translocation was reduced by treatment with HGF. In addition, increased poly(ADP-ribose) polymer formation after NMDA exposure was attenuated by treatment with HGF. These results suggest that the protective effects of HGF against NMDA-induced neurotoxicity are mediated via the partial prevention of caspase 3 activity and the inhibition of AIF translocation to the nucleus. SN - 0022-3042 UR - https://www.unboundmedicine.com/medline/citation/16135073/Inhibition_of_apoptosis_inducing_factor_translocation_is_involved_in_protective_effects_of_hepatocyte_growth_factor_against_excitotoxic_cell_death_in_cultured_hippocampal_neurons_ L2 - https://doi.org/10.1111/j.1471-4159.2005.03446.x DB - PRIME DP - Unbound Medicine ER -