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Isolation and pharmacological characterization of cannitoxin, a presynaptic neurotoxin from the venom of the Papuan Taipan (Oxyuranus scutellatus canni).
J Pharmacol Exp Ther. 2005 Dec; 315(3):1196-202.JP

Abstract

The Papuan taipan (Oxyuranus scutellatus canni) is widely distributed throughout much of Papua New Guinea. Although neurotoxicity is a major symptom of envenomation, no neurotoxins have been isolated from this venom. Using a series of size exclusion chromatography steps, we report the isolation of cannitoxin, a presynaptic neurotoxin (44,848 Da) that represents approximately 16% of the whole venom. The toxin displayed high phospholipase A2 (PLA2 activity (330 +/- 5 micromol/min/mg) and caused concentration-dependent (11-66 nM) inhibition of indirect (0.2 ms; 0.1 Hz; supramaximal V) twitches of the chick biventer cervicis nerve-muscle preparation without effecting nicotinic receptor agonists. Prior addition of CSL Taipan antivenom (5 U/ml) or inhibition of phospholipase A2 activity by incubation with 4-bromophenacyl bromide prevented the inhibition of twitches. Cannitoxin is composed of three different subunits, alpha, beta, and gamma, with the possibility of two beta isomers. However, only the alpha subunit displayed in vitro neurotoxic activity of its own. Thus, cannitoxin is similar in structure and pharmacology to taipoxin, which has been isolated from the closely related Australian species O. scutellatus scutellatus (coastal taipan).

Authors+Show Affiliations

Monash Venom Group, Department of Pharmacology, Monash University, Victoria, Australia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16135698

Citation

Kuruppu, Sanjaya, et al. "Isolation and Pharmacological Characterization of Cannitoxin, a Presynaptic Neurotoxin From the Venom of the Papuan Taipan (Oxyuranus Scutellatus Canni)." The Journal of Pharmacology and Experimental Therapeutics, vol. 315, no. 3, 2005, pp. 1196-202.
Kuruppu S, Reeve S, Banerjee Y, et al. Isolation and pharmacological characterization of cannitoxin, a presynaptic neurotoxin from the venom of the Papuan Taipan (Oxyuranus scutellatus canni). J Pharmacol Exp Ther. 2005;315(3):1196-202.
Kuruppu, S., Reeve, S., Banerjee, Y., Kini, R. M., Smith, A. I., & Hodgson, W. C. (2005). Isolation and pharmacological characterization of cannitoxin, a presynaptic neurotoxin from the venom of the Papuan Taipan (Oxyuranus scutellatus canni). The Journal of Pharmacology and Experimental Therapeutics, 315(3), 1196-202.
Kuruppu S, et al. Isolation and Pharmacological Characterization of Cannitoxin, a Presynaptic Neurotoxin From the Venom of the Papuan Taipan (Oxyuranus Scutellatus Canni). J Pharmacol Exp Ther. 2005;315(3):1196-202. PubMed PMID: 16135698.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation and pharmacological characterization of cannitoxin, a presynaptic neurotoxin from the venom of the Papuan Taipan (Oxyuranus scutellatus canni). AU - Kuruppu,Sanjaya, AU - Reeve,Shane, AU - Banerjee,Yajnavalka, AU - Kini,R Manjunatha, AU - Smith,A Ian, AU - Hodgson,Wayne C, Y1 - 2005/08/31/ PY - 2005/9/2/pubmed PY - 2006/1/24/medline PY - 2005/9/2/entrez SP - 1196 EP - 202 JF - The Journal of pharmacology and experimental therapeutics JO - J. Pharmacol. Exp. Ther. VL - 315 IS - 3 N2 - The Papuan taipan (Oxyuranus scutellatus canni) is widely distributed throughout much of Papua New Guinea. Although neurotoxicity is a major symptom of envenomation, no neurotoxins have been isolated from this venom. Using a series of size exclusion chromatography steps, we report the isolation of cannitoxin, a presynaptic neurotoxin (44,848 Da) that represents approximately 16% of the whole venom. The toxin displayed high phospholipase A2 (PLA2 activity (330 +/- 5 micromol/min/mg) and caused concentration-dependent (11-66 nM) inhibition of indirect (0.2 ms; 0.1 Hz; supramaximal V) twitches of the chick biventer cervicis nerve-muscle preparation without effecting nicotinic receptor agonists. Prior addition of CSL Taipan antivenom (5 U/ml) or inhibition of phospholipase A2 activity by incubation with 4-bromophenacyl bromide prevented the inhibition of twitches. Cannitoxin is composed of three different subunits, alpha, beta, and gamma, with the possibility of two beta isomers. However, only the alpha subunit displayed in vitro neurotoxic activity of its own. Thus, cannitoxin is similar in structure and pharmacology to taipoxin, which has been isolated from the closely related Australian species O. scutellatus scutellatus (coastal taipan). SN - 0022-3565 UR - https://www.unboundmedicine.com/medline/citation/16135698/Isolation_and_pharmacological_characterization_of_cannitoxin_a_presynaptic_neurotoxin_from_the_venom_of_the_Papuan_Taipan__Oxyuranus_scutellatus_canni__ L2 - http://jpet.aspetjournals.org/cgi/pmidlookup?view=long&pmid=16135698 DB - PRIME DP - Unbound Medicine ER -